Defects in tangential neuronal migration of pontine nuclei neurons in the Largemyd mouse are associated with stalled migration in the ventrolateral hindbrain

Qiang Qu, James E. Crandall, Tuanlian Luo, Peter J. McCaffery, Frances I. Smith

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The LARGE gene encodes a putative glycosyltransferase that is required for normal glycosylation of dystroglycan, and defects in LARGE can cause abnormal neuronal migration in congenital muscular dystrophy (CMD). Previous studies have focused on radial migration, which is disrupted at least in part due to breaks in the basal lamina. Through analysis of precerebellar nuclei development in the Large(myd) mouse hindbrain, we show that tangential migration of a subgroup of hindbrain neurons may also be disrupted. Within the precerebellar nuclei, the pontine nuclei (PN) are severely disrupted, whereas the inferior olive (IO), external cuneate nuclei (ECN) and lateral reticular nuclei (LRN) appear unaffected. Large and dystroglycan are widely expressed in the hindbrain, including in the pontine neurons migrating in the anterior extramural migratory stream (AES). BrdU labeling and immunohistochemical studies suggest normal numbers of neurons begin their journey towards the ventral midline in the AES in the Large(myd) mouse. However, migration stalls and PN neurons fail to reach the midline, surviving as ectopic clusters of cells located under the pial surface dorsally and laterally to where they normally would finish their migration near the ventral midline. Stalling of PN neurons at this location is also observed in other migration disorders in mice. These observations suggest that glycan-dependent dystroglycan interactions are required for PN neurons to correctly respond to signals at this important migrational checkpoint.
Original languageEnglish
Pages (from-to)2877-2886
Number of pages10
JournalEuropean Journal of Neuroscience
Volume23
Issue number11
DOIs
Publication statusPublished - 1 Jun 2006

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Rhombencephalon
Dystroglycans
Neurons
Glycosyltransferases
Medulla Oblongata
Muscular Dystrophies
Bromodeoxyuridine
Glycosylation
Basement Membrane
Polysaccharides
Genes

Keywords

  • Amino Acids
  • Animals
  • Animals, Newborn
  • Bromodeoxyuridine
  • Caspase 3
  • Caspases
  • Cell Movement
  • Dystroglycans
  • Embryo, Mammalian
  • Eye Proteins
  • Gene Expression Regulation, Developmental
  • Glycosyltransferases
  • Homeodomain Proteins
  • Immunohistochemistry
  • In Situ Hybridization
  • Intermediate Filaments
  • Laminin
  • Mice
  • Mice, Transgenic
  • Neural Pathways
  • Neurons
  • Paired Box Transcription Factors
  • RNA, Messenger
  • Repressor Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rhombencephalon

Cite this

Defects in tangential neuronal migration of pontine nuclei neurons in the Largemyd mouse are associated with stalled migration in the ventrolateral hindbrain. / Qu, Qiang; Crandall, James E.; Luo, Tuanlian; McCaffery, Peter J.; Smith, Frances I.

In: European Journal of Neuroscience, Vol. 23, No. 11, 01.06.2006, p. 2877-2886.

Research output: Contribution to journalArticle

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abstract = "The LARGE gene encodes a putative glycosyltransferase that is required for normal glycosylation of dystroglycan, and defects in LARGE can cause abnormal neuronal migration in congenital muscular dystrophy (CMD). Previous studies have focused on radial migration, which is disrupted at least in part due to breaks in the basal lamina. Through analysis of precerebellar nuclei development in the Large(myd) mouse hindbrain, we show that tangential migration of a subgroup of hindbrain neurons may also be disrupted. Within the precerebellar nuclei, the pontine nuclei (PN) are severely disrupted, whereas the inferior olive (IO), external cuneate nuclei (ECN) and lateral reticular nuclei (LRN) appear unaffected. Large and dystroglycan are widely expressed in the hindbrain, including in the pontine neurons migrating in the anterior extramural migratory stream (AES). BrdU labeling and immunohistochemical studies suggest normal numbers of neurons begin their journey towards the ventral midline in the AES in the Large(myd) mouse. However, migration stalls and PN neurons fail to reach the midline, surviving as ectopic clusters of cells located under the pial surface dorsally and laterally to where they normally would finish their migration near the ventral midline. Stalling of PN neurons at this location is also observed in other migration disorders in mice. These observations suggest that glycan-dependent dystroglycan interactions are required for PN neurons to correctly respond to signals at this important migrational checkpoint.",
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T1 - Defects in tangential neuronal migration of pontine nuclei neurons in the Largemyd mouse are associated with stalled migration in the ventrolateral hindbrain

AU - Qu, Qiang

AU - Crandall, James E.

AU - Luo, Tuanlian

AU - McCaffery, Peter J.

AU - Smith, Frances I.

PY - 2006/6/1

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N2 - The LARGE gene encodes a putative glycosyltransferase that is required for normal glycosylation of dystroglycan, and defects in LARGE can cause abnormal neuronal migration in congenital muscular dystrophy (CMD). Previous studies have focused on radial migration, which is disrupted at least in part due to breaks in the basal lamina. Through analysis of precerebellar nuclei development in the Large(myd) mouse hindbrain, we show that tangential migration of a subgroup of hindbrain neurons may also be disrupted. Within the precerebellar nuclei, the pontine nuclei (PN) are severely disrupted, whereas the inferior olive (IO), external cuneate nuclei (ECN) and lateral reticular nuclei (LRN) appear unaffected. Large and dystroglycan are widely expressed in the hindbrain, including in the pontine neurons migrating in the anterior extramural migratory stream (AES). BrdU labeling and immunohistochemical studies suggest normal numbers of neurons begin their journey towards the ventral midline in the AES in the Large(myd) mouse. However, migration stalls and PN neurons fail to reach the midline, surviving as ectopic clusters of cells located under the pial surface dorsally and laterally to where they normally would finish their migration near the ventral midline. Stalling of PN neurons at this location is also observed in other migration disorders in mice. These observations suggest that glycan-dependent dystroglycan interactions are required for PN neurons to correctly respond to signals at this important migrational checkpoint.

AB - The LARGE gene encodes a putative glycosyltransferase that is required for normal glycosylation of dystroglycan, and defects in LARGE can cause abnormal neuronal migration in congenital muscular dystrophy (CMD). Previous studies have focused on radial migration, which is disrupted at least in part due to breaks in the basal lamina. Through analysis of precerebellar nuclei development in the Large(myd) mouse hindbrain, we show that tangential migration of a subgroup of hindbrain neurons may also be disrupted. Within the precerebellar nuclei, the pontine nuclei (PN) are severely disrupted, whereas the inferior olive (IO), external cuneate nuclei (ECN) and lateral reticular nuclei (LRN) appear unaffected. Large and dystroglycan are widely expressed in the hindbrain, including in the pontine neurons migrating in the anterior extramural migratory stream (AES). BrdU labeling and immunohistochemical studies suggest normal numbers of neurons begin their journey towards the ventral midline in the AES in the Large(myd) mouse. However, migration stalls and PN neurons fail to reach the midline, surviving as ectopic clusters of cells located under the pial surface dorsally and laterally to where they normally would finish their migration near the ventral midline. Stalling of PN neurons at this location is also observed in other migration disorders in mice. These observations suggest that glycan-dependent dystroglycan interactions are required for PN neurons to correctly respond to signals at this important migrational checkpoint.

KW - Amino Acids

KW - Animals

KW - Animals, Newborn

KW - Bromodeoxyuridine

KW - Caspase 3

KW - Caspases

KW - Cell Movement

KW - Dystroglycans

KW - Embryo, Mammalian

KW - Eye Proteins

KW - Gene Expression Regulation, Developmental

KW - Glycosyltransferases

KW - Homeodomain Proteins

KW - Immunohistochemistry

KW - In Situ Hybridization

KW - Intermediate Filaments

KW - Laminin

KW - Mice

KW - Mice, Transgenic

KW - Neural Pathways

KW - Neurons

KW - Paired Box Transcription Factors

KW - RNA, Messenger

KW - Repressor Proteins

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Rhombencephalon

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DO - 10.1111/j.1460-9568.2006.04836.x

M3 - Article

VL - 23

SP - 2877

EP - 2886

JO - European Journal of Neuroscience

JF - European Journal of Neuroscience

SN - 0953-816X

IS - 11

ER -