Degradation of metalaxyl-M in contrasting soils is influenced more by differences in physicochemical characteristics than in microbial community composition after re-inoculation of sterilised soils

Kate L. Baker, Samantha Marshall, Graeme W. Nicol, Colin D. Campbell, Gilles Nicollier, Dean Ricketts, Kenneth Killham, James I. Prosser

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Rates of degradation of pesticides by soil microorganisms are believed to depend on both microbial community composition and underlying soil physicochemical characteristics. The aim of this study was to determine which of these factors was more important in determining the rate of degradation of the fungicide metalaxyl-M in two soils. Soils exhibiting highly contrasting metalaxyl-M degradation rates were sterilised by gamma-irradiation and inoculated with either non-sterilised soil from the same site or with the soil from the contrasting site. After re-establishment of microbial communities, soils were treated with metalaxyl-M and the degradation rate (measured by C-14-HPLC), pH and microbial community structure (multiplex terminal-restriction fragment length polymorphism (T-RFLP) analysis of small subunit rRNA gene sequences) were assessed. Community composition was altered by the sterilisation and re-inoculation strategy but degradation in re-inoculated soils was still most rapid in the soil with the original faster degradation rate. This was the case regardless of the source of the soil inoculum. and the rate of degradation in the soil exhibiting the low natural degradation rate remained low when inoculated with the faster-degrading soil. The results suggest that while the slower-degrading soil possessed a degradative capacity, the degradation rate in this soil was significantly reduced by some of its physicochemical characteristics, despite introduction of the microbial community of the faster-degrading soil. These results and this experimental strategy provide a basis for the assessment of relative importance of the factors limiting biodegradation and management strategies required to enhance degradation rates.

Original languageEnglish
Pages (from-to)1123-1131
Number of pages9
JournalSoil Biology and Biochemistry
Volume42
Issue number7
Early online date3 Apr 2010
DOIs
Publication statusPublished - Jul 2010

Keywords

  • T-RFLP
  • fungicide degradation
  • metalaxyl-M
  • soil microbial community composition
  • community swap
  • sterilisation-re-inoculation
  • acute gamma-irradiation
  • bacterial communities
  • spatial variability
  • organic-matter
  • arable soils
  • forest soil
  • biodegradation
  • pH
  • persistence
  • microorganisms

Cite this

Degradation of metalaxyl-M in contrasting soils is influenced more by differences in physicochemical characteristics than in microbial community composition after re-inoculation of sterilised soils. / Baker, Kate L.; Marshall, Samantha; Nicol, Graeme W.; Campbell, Colin D.; Nicollier, Gilles; Ricketts, Dean; Killham, Kenneth; Prosser, James I.

In: Soil Biology and Biochemistry, Vol. 42, No. 7, 07.2010, p. 1123-1131.

Research output: Contribution to journalArticle

Baker, Kate L. ; Marshall, Samantha ; Nicol, Graeme W. ; Campbell, Colin D. ; Nicollier, Gilles ; Ricketts, Dean ; Killham, Kenneth ; Prosser, James I. / Degradation of metalaxyl-M in contrasting soils is influenced more by differences in physicochemical characteristics than in microbial community composition after re-inoculation of sterilised soils. In: Soil Biology and Biochemistry. 2010 ; Vol. 42, No. 7. pp. 1123-1131.
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abstract = "Rates of degradation of pesticides by soil microorganisms are believed to depend on both microbial community composition and underlying soil physicochemical characteristics. The aim of this study was to determine which of these factors was more important in determining the rate of degradation of the fungicide metalaxyl-M in two soils. Soils exhibiting highly contrasting metalaxyl-M degradation rates were sterilised by gamma-irradiation and inoculated with either non-sterilised soil from the same site or with the soil from the contrasting site. After re-establishment of microbial communities, soils were treated with metalaxyl-M and the degradation rate (measured by C-14-HPLC), pH and microbial community structure (multiplex terminal-restriction fragment length polymorphism (T-RFLP) analysis of small subunit rRNA gene sequences) were assessed. Community composition was altered by the sterilisation and re-inoculation strategy but degradation in re-inoculated soils was still most rapid in the soil with the original faster degradation rate. This was the case regardless of the source of the soil inoculum. and the rate of degradation in the soil exhibiting the low natural degradation rate remained low when inoculated with the faster-degrading soil. The results suggest that while the slower-degrading soil possessed a degradative capacity, the degradation rate in this soil was significantly reduced by some of its physicochemical characteristics, despite introduction of the microbial community of the faster-degrading soil. These results and this experimental strategy provide a basis for the assessment of relative importance of the factors limiting biodegradation and management strategies required to enhance degradation rates.",
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T1 - Degradation of metalaxyl-M in contrasting soils is influenced more by differences in physicochemical characteristics than in microbial community composition after re-inoculation of sterilised soils

AU - Baker, Kate L.

AU - Marshall, Samantha

AU - Nicol, Graeme W.

AU - Campbell, Colin D.

AU - Nicollier, Gilles

AU - Ricketts, Dean

AU - Killham, Kenneth

AU - Prosser, James I.

PY - 2010/7

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N2 - Rates of degradation of pesticides by soil microorganisms are believed to depend on both microbial community composition and underlying soil physicochemical characteristics. The aim of this study was to determine which of these factors was more important in determining the rate of degradation of the fungicide metalaxyl-M in two soils. Soils exhibiting highly contrasting metalaxyl-M degradation rates were sterilised by gamma-irradiation and inoculated with either non-sterilised soil from the same site or with the soil from the contrasting site. After re-establishment of microbial communities, soils were treated with metalaxyl-M and the degradation rate (measured by C-14-HPLC), pH and microbial community structure (multiplex terminal-restriction fragment length polymorphism (T-RFLP) analysis of small subunit rRNA gene sequences) were assessed. Community composition was altered by the sterilisation and re-inoculation strategy but degradation in re-inoculated soils was still most rapid in the soil with the original faster degradation rate. This was the case regardless of the source of the soil inoculum. and the rate of degradation in the soil exhibiting the low natural degradation rate remained low when inoculated with the faster-degrading soil. The results suggest that while the slower-degrading soil possessed a degradative capacity, the degradation rate in this soil was significantly reduced by some of its physicochemical characteristics, despite introduction of the microbial community of the faster-degrading soil. These results and this experimental strategy provide a basis for the assessment of relative importance of the factors limiting biodegradation and management strategies required to enhance degradation rates.

AB - Rates of degradation of pesticides by soil microorganisms are believed to depend on both microbial community composition and underlying soil physicochemical characteristics. The aim of this study was to determine which of these factors was more important in determining the rate of degradation of the fungicide metalaxyl-M in two soils. Soils exhibiting highly contrasting metalaxyl-M degradation rates were sterilised by gamma-irradiation and inoculated with either non-sterilised soil from the same site or with the soil from the contrasting site. After re-establishment of microbial communities, soils were treated with metalaxyl-M and the degradation rate (measured by C-14-HPLC), pH and microbial community structure (multiplex terminal-restriction fragment length polymorphism (T-RFLP) analysis of small subunit rRNA gene sequences) were assessed. Community composition was altered by the sterilisation and re-inoculation strategy but degradation in re-inoculated soils was still most rapid in the soil with the original faster degradation rate. This was the case regardless of the source of the soil inoculum. and the rate of degradation in the soil exhibiting the low natural degradation rate remained low when inoculated with the faster-degrading soil. The results suggest that while the slower-degrading soil possessed a degradative capacity, the degradation rate in this soil was significantly reduced by some of its physicochemical characteristics, despite introduction of the microbial community of the faster-degrading soil. These results and this experimental strategy provide a basis for the assessment of relative importance of the factors limiting biodegradation and management strategies required to enhance degradation rates.

KW - T-RFLP

KW - fungicide degradation

KW - metalaxyl-M

KW - soil microbial community composition

KW - community swap

KW - sterilisation-re-inoculation

KW - acute gamma-irradiation

KW - bacterial communities

KW - spatial variability

KW - organic-matter

KW - arable soils

KW - forest soil

KW - biodegradation

KW - pH

KW - persistence

KW - microorganisms

U2 - 10.1016/j.soilbio.2010.03.016

DO - 10.1016/j.soilbio.2010.03.016

M3 - Article

VL - 42

SP - 1123

EP - 1131

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

IS - 7

ER -