Degradation of the polycyclic aromatic hydrocarbon (PAH) fluorene is retarded in a Scots pine ectomycorrhizosphere

David R Genney, Kenneth Stuart Killham, Ian James Alexander, Andrew Alexander Meharg

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are an important class of persistent organic pollutants (POPs) in the environment and accumulate in forest soils. These soils are often dominated by ectomycorrhizal (EcM) roots, but little is known about how EcM fungi degrade PAHs, or the overall effect of field colonized EcM roots on the fate of PAHs.

The ability of eight EcM fungi to degrade PAHs in liquid culture spiked with C-14 labelled PAHs was investigated. Microcosms were used to determine the impact of naturally colonized mycorrhizal pine seedlings on PAH mineralization and volatilization.

Only two EcM fungi (Thelephora terrestris and Laccaria laccata) degraded at least one PAH and none were able to mineralize the PAHs in pure culture. Where degradation occurred, the compounds were only mono-oxygenated. EcM pine seedlings did not alter naphthalene mineralization or volatilization but retarded fluorene mineralization by 35% compared with unplanted, ectomycorrhizosphere soil inoculated, microcosms.

The EcM fungi possessed limited PAH degrading abilities, which may explain why EcM dominated microcosms retarded fluorene mineralization. This observation is considered in relation to the 'Gadgil-effect', where retarded litter decomposition has been observed in the presence of EcM roots.

Original languageEnglish
Pages (from-to)641-649
Number of pages8
JournalNew Phytologist
Volume163
DOIs
Publication statusPublished - 2004

Keywords

  • ectomycorrhizal fungus
  • PAH
  • Gadgil-effect
  • Pinus sylvestris
  • degradation
  • WHITE-ROT FUNGI
  • PLEUROTUS-OSTREATUS
  • MYCORRHIZAL FUNGI
  • LIGNIN PEROXIDASE
  • SOIL
  • PYRENE
  • MINERALIZATION
  • BIODEGRADATION
  • DECOMPOSITION
  • PSEUDOMONADS

Cite this

Degradation of the polycyclic aromatic hydrocarbon (PAH) fluorene is retarded in a Scots pine ectomycorrhizosphere. / Genney, David R; Killham, Kenneth Stuart; Alexander, Ian James; Meharg, Andrew Alexander.

In: New Phytologist, Vol. 163, 2004, p. 641-649.

Research output: Contribution to journalArticle

Genney, David R ; Killham, Kenneth Stuart ; Alexander, Ian James ; Meharg, Andrew Alexander. / Degradation of the polycyclic aromatic hydrocarbon (PAH) fluorene is retarded in a Scots pine ectomycorrhizosphere. In: New Phytologist. 2004 ; Vol. 163. pp. 641-649.
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AB - Polycyclic aromatic hydrocarbons (PAHs) are an important class of persistent organic pollutants (POPs) in the environment and accumulate in forest soils. These soils are often dominated by ectomycorrhizal (EcM) roots, but little is known about how EcM fungi degrade PAHs, or the overall effect of field colonized EcM roots on the fate of PAHs.The ability of eight EcM fungi to degrade PAHs in liquid culture spiked with C-14 labelled PAHs was investigated. Microcosms were used to determine the impact of naturally colonized mycorrhizal pine seedlings on PAH mineralization and volatilization.Only two EcM fungi (Thelephora terrestris and Laccaria laccata) degraded at least one PAH and none were able to mineralize the PAHs in pure culture. Where degradation occurred, the compounds were only mono-oxygenated. EcM pine seedlings did not alter naphthalene mineralization or volatilization but retarded fluorene mineralization by 35% compared with unplanted, ectomycorrhizosphere soil inoculated, microcosms.The EcM fungi possessed limited PAH degrading abilities, which may explain why EcM dominated microcosms retarded fluorene mineralization. This observation is considered in relation to the 'Gadgil-effect', where retarded litter decomposition has been observed in the presence of EcM roots.

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