Delayed apoptosis in patients with multiple organ dysfunction syndrome

Noha El Sakka, Helen Frances Galley, O Sharaki, M Helmy, S Marsouk, Shafik Azmy, M Sedrack, Nigel Robert Webster

Research output: Contribution to journalArticle

Abstract

Aged neutrophils undergo spontaneous apoptosis and delayed apoptosis is associated with persistence of inflammatory disorders through release of toxic metabolites. We evaluated spontaneous apoptosis of neutrophils in patients with multiorgan dysfunction syndrome and neutrophil respiratory burst activity. Neutrophil apoptosis was assessed at study enrollment and after 24 hours incubation in culture medium by annexin-V assay, morphology and DNA fragmentation. Respiratory burst activity was measured using dihydrorhodamine. Twenty two patients with multiple organ dysfunction syndrome admitted to an intensive care unit and 22 healthy controls were studied. After 24 hours, a profound delay in spontaneous apoptosis in organ dysfunction patients was seen compared to controls for both annexin-V (26.9% versus 52.1%, p< 0.0001) and morphological assessment (25.0% versus 61.5%, p<0.0001). Respiratory burst activity was increased (86.3% versus 27.6%, p<0.001) suggesting that the delay in apoptosis was associated with prolongation of the functional activity of the cells. We conclude that in patient with multiple organ dysfunction syndrome there is a delay in spontaneous apoptosis together with a high functional activity of neutrophils.
Original languageEnglish
Pages (from-to)9-15
JournalCritical Care and Shock
Volume9
Publication statusPublished - 2006

Fingerprint

Multiple Organ Failure
Apoptosis
Neutrophils
Respiratory Burst
Annexin A5
Poisons
DNA Fragmentation
Intensive Care Units
Culture Media

Keywords

  • apoptosis
  • multiorgan dysfunction
  • sepsis
  • neutrophils

Cite this

El Sakka, N., Galley, H. F., Sharaki, O., Helmy, M., Marsouk, S., Azmy, S., ... Webster, N. R. (2006). Delayed apoptosis in patients with multiple organ dysfunction syndrome. Critical Care and Shock, 9, 9-15.

Delayed apoptosis in patients with multiple organ dysfunction syndrome. / El Sakka, Noha; Galley, Helen Frances; Sharaki, O; Helmy, M; Marsouk, S; Azmy, Shafik; Sedrack, M; Webster, Nigel Robert.

In: Critical Care and Shock, Vol. 9, 2006, p. 9-15.

Research output: Contribution to journalArticle

El Sakka, N, Galley, HF, Sharaki, O, Helmy, M, Marsouk, S, Azmy, S, Sedrack, M & Webster, NR 2006, 'Delayed apoptosis in patients with multiple organ dysfunction syndrome', Critical Care and Shock, vol. 9, pp. 9-15.
El Sakka N, Galley HF, Sharaki O, Helmy M, Marsouk S, Azmy S et al. Delayed apoptosis in patients with multiple organ dysfunction syndrome. Critical Care and Shock. 2006;9:9-15.
El Sakka, Noha ; Galley, Helen Frances ; Sharaki, O ; Helmy, M ; Marsouk, S ; Azmy, Shafik ; Sedrack, M ; Webster, Nigel Robert. / Delayed apoptosis in patients with multiple organ dysfunction syndrome. In: Critical Care and Shock. 2006 ; Vol. 9. pp. 9-15.
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AU - Webster, Nigel Robert

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AB - Aged neutrophils undergo spontaneous apoptosis and delayed apoptosis is associated with persistence of inflammatory disorders through release of toxic metabolites. We evaluated spontaneous apoptosis of neutrophils in patients with multiorgan dysfunction syndrome and neutrophil respiratory burst activity. Neutrophil apoptosis was assessed at study enrollment and after 24 hours incubation in culture medium by annexin-V assay, morphology and DNA fragmentation. Respiratory burst activity was measured using dihydrorhodamine. Twenty two patients with multiple organ dysfunction syndrome admitted to an intensive care unit and 22 healthy controls were studied. After 24 hours, a profound delay in spontaneous apoptosis in organ dysfunction patients was seen compared to controls for both annexin-V (26.9% versus 52.1%, p< 0.0001) and morphological assessment (25.0% versus 61.5%, p<0.0001). Respiratory burst activity was increased (86.3% versus 27.6%, p<0.001) suggesting that the delay in apoptosis was associated with prolongation of the functional activity of the cells. We conclude that in patient with multiple organ dysfunction syndrome there is a delay in spontaneous apoptosis together with a high functional activity of neutrophils.

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