TY - JOUR
T1 - Deletion of the epigenetic regulator GcnE in Aspergillus niger FGSC A1279 activates the production of multiple polyketide metabolites
AU - Wang, Bin
AU - Li, Xuejie
AU - Yu, Dou
AU - Chen, Xiaoyi
AU - Tabudravu, Jioji
AU - Deng, Hai
AU - Pan, Li
N1 - Acknowledgements
This work was supported by the following Chinese fundings: The Natural Science Foundation of Guangdong Province (grant number 2017A30313097), the Science and Technology Planning Project of Guangdong Province (grant numbers 2016A050503016 and 2016A010105004), the Science and Technology Planning Project of Guangzhou City (grant number 201510010191), the Fundamental Research Funds for the Central Universities (grant number 2015ZP032), and the China Scholarship Council (CSC) fund (grant number 201606155032).
PY - 2018/12/31
Y1 - 2018/12/31
N2 - Epigenetic modification is an important regulatory mechanism in the biosynthesis of secondary metabolites in Aspergillus species, which have been considered to be the treasure trove of new bioactive secondary metabolites. In this study, we reported that deletion of the epigenetic regulator gcnE, a histone acetyltransferase in the SAGA/ADA complex, resulted in the production of 12 polyketide secondary metabolites in A. niger FGSC A1279, which was previously not known to produce toxins or secondary metabolites. Chemical workup and structural elucidation by 1D/2D NMR and high resolution electrospray ionization mass (HR-ESIMS) yielded the novel compound nigerpyrone (1) and five known compounds: carbonarone A (2), pestalamide A (3), funalenone (4), aurasperone E (5), and aurasperone A (6). Based on chemical information and the literature, the biosynthetic gene clusters of funalenone (4), aurasperone E (5), and aurasperone A (6) were located on chromosomes of A. niger FGSC A1279. This study found that inactivation of GcnE activated the production of secondary metabolites in A. niger. The biosynthetic pathway for nigerpyrone and its derivatives was identified and characterized via gene knockout and complementation experiments. A biosynthetic model of this group of pyran-based fungal metabolites was proposed.
AB - Epigenetic modification is an important regulatory mechanism in the biosynthesis of secondary metabolites in Aspergillus species, which have been considered to be the treasure trove of new bioactive secondary metabolites. In this study, we reported that deletion of the epigenetic regulator gcnE, a histone acetyltransferase in the SAGA/ADA complex, resulted in the production of 12 polyketide secondary metabolites in A. niger FGSC A1279, which was previously not known to produce toxins or secondary metabolites. Chemical workup and structural elucidation by 1D/2D NMR and high resolution electrospray ionization mass (HR-ESIMS) yielded the novel compound nigerpyrone (1) and five known compounds: carbonarone A (2), pestalamide A (3), funalenone (4), aurasperone E (5), and aurasperone A (6). Based on chemical information and the literature, the biosynthetic gene clusters of funalenone (4), aurasperone E (5), and aurasperone A (6) were located on chromosomes of A. niger FGSC A1279. This study found that inactivation of GcnE activated the production of secondary metabolites in A. niger. The biosynthetic pathway for nigerpyrone and its derivatives was identified and characterized via gene knockout and complementation experiments. A biosynthetic model of this group of pyran-based fungal metabolites was proposed.
KW - Epigenetic regulator
KW - Histone acetyltransferase GcnE
KW - Nigerpyrone
KW - Polyketide
KW - Secondary metabolite
UR - http://www.scopus.com/inward/record.url?scp=85055335923&partnerID=8YFLogxK
U2 - 10.1016/j.micres.2018.10.004
DO - 10.1016/j.micres.2018.10.004
M3 - Article
C2 - 30384904
AN - SCOPUS:85055335923
VL - 217
SP - 101
EP - 107
JO - Microbiological Research
JF - Microbiological Research
SN - 0944-5013
ER -