Deletion of the membrane complement inhibitor CD59a drives age and gender-dependent alterations to bone phenotype in mice

Anja C. Bloom, Fraser L. Collins, Rob J. van't Hof, Elizabeth S. Ryan, Emma Jones, Timothy R. Hughes, B. Paul Morgan, Malin Erlandsson, Maria Bokarewa, Daniel Aeschlimann, Bronwen A.J. Evans, Anwen S. Williams

Research output: Contribution to journalArticle

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Abstract

Degenerative joint diseases such as osteoarthritis are characterised by aberrant region-specific bone formation and abnormal bone mineral content. A recent study suggested a role for the complement membrane attack complex in experimental models of osteoarthritis. Since CD59a is the principal regulator of the membrane attack complex in mice, we evaluated the impact of CD59a gene deletion upon maintenance of bone architecture.In vivo bone morphology analysis revealed that male CD59a-deficient mice have increased femur length and cortical bone volume, albeit with reduced bone mineral density. However, this phenomenon was not observed in female mice. Histomorphometric analysis of the trabecular bone showed increased rates of bone homeostasis, with both increased bone resorption and mineral apposition rate in CD59a-deficient male mice. When bone cells were studied in isolation, in vitro osteoclastogenesis was significantly increased in male CD59a-deficient mice, although osteoblast formation was not altered.Our data reveal, for the first time, that CD59a is a regulator of bone growth and homeostasis. CD59a ablation in male mice results in longer and wider bones, but with less density, which is likely a major contributing factor for their susceptibility to osteoarthritis. These findings increase our understanding of the role of complement regulation in degenerative arthritis.
Original languageEnglish
Pages (from-to)253-261
Number of pages9
JournalBone
Volume84
Early online date22 Dec 2015
DOIs
Publication statusPublished - 1 Mar 2016

Fingerprint

Complement Inactivating Agents
Osteoarthritis
Phenotype
Bone and Bones
Membranes
Complement Membrane Attack Complex
Osteogenesis
Bone Density
Homeostasis
Bone Development
Gene Deletion
Bone Resorption
Osteoblasts
Femur
Minerals
Theoretical Models
Maintenance

Keywords

  • Ageing
  • Bone
  • CD59a
  • Micro-CT
  • Osteoclast

Cite this

Deletion of the membrane complement inhibitor CD59a drives age and gender-dependent alterations to bone phenotype in mice. / Bloom, Anja C.; Collins, Fraser L.; van't Hof, Rob J.; Ryan, Elizabeth S.; Jones, Emma; Hughes, Timothy R.; Morgan, B. Paul; Erlandsson, Malin; Bokarewa, Maria; Aeschlimann, Daniel; Evans, Bronwen A.J.; Williams, Anwen S.

In: Bone, Vol. 84, 01.03.2016, p. 253-261.

Research output: Contribution to journalArticle

Bloom, AC, Collins, FL, van't Hof, RJ, Ryan, ES, Jones, E, Hughes, TR, Morgan, BP, Erlandsson, M, Bokarewa, M, Aeschlimann, D, Evans, BAJ & Williams, AS 2016, 'Deletion of the membrane complement inhibitor CD59a drives age and gender-dependent alterations to bone phenotype in mice', Bone, vol. 84, pp. 253-261. https://doi.org/10.1016/j.bone.2015.12.014
Bloom, Anja C. ; Collins, Fraser L. ; van't Hof, Rob J. ; Ryan, Elizabeth S. ; Jones, Emma ; Hughes, Timothy R. ; Morgan, B. Paul ; Erlandsson, Malin ; Bokarewa, Maria ; Aeschlimann, Daniel ; Evans, Bronwen A.J. ; Williams, Anwen S. / Deletion of the membrane complement inhibitor CD59a drives age and gender-dependent alterations to bone phenotype in mice. In: Bone. 2016 ; Vol. 84. pp. 253-261.
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abstract = "Degenerative joint diseases such as osteoarthritis are characterised by aberrant region-specific bone formation and abnormal bone mineral content. A recent study suggested a role for the complement membrane attack complex in experimental models of osteoarthritis. Since CD59a is the principal regulator of the membrane attack complex in mice, we evaluated the impact of CD59a gene deletion upon maintenance of bone architecture.In vivo bone morphology analysis revealed that male CD59a-deficient mice have increased femur length and cortical bone volume, albeit with reduced bone mineral density. However, this phenomenon was not observed in female mice. Histomorphometric analysis of the trabecular bone showed increased rates of bone homeostasis, with both increased bone resorption and mineral apposition rate in CD59a-deficient male mice. When bone cells were studied in isolation, in vitro osteoclastogenesis was significantly increased in male CD59a-deficient mice, although osteoblast formation was not altered.Our data reveal, for the first time, that CD59a is a regulator of bone growth and homeostasis. CD59a ablation in male mice results in longer and wider bones, but with less density, which is likely a major contributing factor for their susceptibility to osteoarthritis. These findings increase our understanding of the role of complement regulation in degenerative arthritis.",
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AU - Evans, Bronwen A.J.

AU - Williams, Anwen S.

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