Delivery of a Salmonella Typhi exotoxin from a host intracellular compartment

Stefania Spanò, Juan E Ugalde, Jorge E Galán

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Salmonella Typhi, an exclusive human pathogen and the cause of typhoid fever, expresses a functional cytolethal distending toxin for which only the active subunit, CdtB, has been identified. Here, we show that PltA and PltB, which are encoded in the same pathogenicity islet as cdtB, associate with CdtB to form a multipartite toxin. PltA and PltB are homologs of components of the pertussis toxin, including its ADP-ribosyl transferase subunit. We also show that PltA and PltB are required for the delivery of CdtB from an intracellular compartment to target cells via autocrine and paracrine pathways. We hypothesize that this toxin, which we have named "typhoid toxin," and its delivery mechanism may contribute to S. Typhi's unique virulence properties.
Original languageEnglish
Pages (from-to)30-38
Number of pages9
JournalCell Host & Microbe
Volume3
Issue number1
Early online date16 Jan 2008
DOIs
Publication statusPublished - 17 Jan 2008

Fingerprint

Exotoxins
Salmonella typhi
Typhoid Fever
Genomic Islands
Pertussis Toxin
Transferases
Adenosine Diphosphate
Virulence
cytolethal distending toxin

Keywords

  • bacterial toxins
  • cell line
  • exotoxins
  • gene expression regulation, bacterial
  • humans
  • intestines
  • phagosomes
  • Salmonella typhi
  • vacuoles
  • virulence
  • MICROBIO
  • CELLBIO

Cite this

Delivery of a Salmonella Typhi exotoxin from a host intracellular compartment. / Spanò, Stefania; Ugalde, Juan E; Galán, Jorge E.

In: Cell Host & Microbe, Vol. 3, No. 1, 17.01.2008, p. 30-38.

Research output: Contribution to journalArticle

Spanò, Stefania ; Ugalde, Juan E ; Galán, Jorge E. / Delivery of a Salmonella Typhi exotoxin from a host intracellular compartment. In: Cell Host & Microbe. 2008 ; Vol. 3, No. 1. pp. 30-38.
@article{cbb91d1ee41c4fc6a57f912fd183c25b,
title = "Delivery of a Salmonella Typhi exotoxin from a host intracellular compartment",
abstract = "Salmonella Typhi, an exclusive human pathogen and the cause of typhoid fever, expresses a functional cytolethal distending toxin for which only the active subunit, CdtB, has been identified. Here, we show that PltA and PltB, which are encoded in the same pathogenicity islet as cdtB, associate with CdtB to form a multipartite toxin. PltA and PltB are homologs of components of the pertussis toxin, including its ADP-ribosyl transferase subunit. We also show that PltA and PltB are required for the delivery of CdtB from an intracellular compartment to target cells via autocrine and paracrine pathways. We hypothesize that this toxin, which we have named {"}typhoid toxin,{"} and its delivery mechanism may contribute to S. Typhi's unique virulence properties.",
keywords = "bacterial toxins, cell line, exotoxins, gene expression regulation, bacterial, humans, intestines, phagosomes, Salmonella typhi, vacuoles, virulence, MICROBIO, CELLBIO",
author = "Stefania Span{\`o} and Ugalde, {Juan E} and Gal{\'a}n, {Jorge E}",
year = "2008",
month = "1",
day = "17",
doi = "10.1016/j.chom.2007.11.001",
language = "English",
volume = "3",
pages = "30--38",
journal = "Cell Host & Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - Delivery of a Salmonella Typhi exotoxin from a host intracellular compartment

AU - Spanò, Stefania

AU - Ugalde, Juan E

AU - Galán, Jorge E

PY - 2008/1/17

Y1 - 2008/1/17

N2 - Salmonella Typhi, an exclusive human pathogen and the cause of typhoid fever, expresses a functional cytolethal distending toxin for which only the active subunit, CdtB, has been identified. Here, we show that PltA and PltB, which are encoded in the same pathogenicity islet as cdtB, associate with CdtB to form a multipartite toxin. PltA and PltB are homologs of components of the pertussis toxin, including its ADP-ribosyl transferase subunit. We also show that PltA and PltB are required for the delivery of CdtB from an intracellular compartment to target cells via autocrine and paracrine pathways. We hypothesize that this toxin, which we have named "typhoid toxin," and its delivery mechanism may contribute to S. Typhi's unique virulence properties.

AB - Salmonella Typhi, an exclusive human pathogen and the cause of typhoid fever, expresses a functional cytolethal distending toxin for which only the active subunit, CdtB, has been identified. Here, we show that PltA and PltB, which are encoded in the same pathogenicity islet as cdtB, associate with CdtB to form a multipartite toxin. PltA and PltB are homologs of components of the pertussis toxin, including its ADP-ribosyl transferase subunit. We also show that PltA and PltB are required for the delivery of CdtB from an intracellular compartment to target cells via autocrine and paracrine pathways. We hypothesize that this toxin, which we have named "typhoid toxin," and its delivery mechanism may contribute to S. Typhi's unique virulence properties.

KW - bacterial toxins

KW - cell line

KW - exotoxins

KW - gene expression regulation, bacterial

KW - humans

KW - intestines

KW - phagosomes

KW - Salmonella typhi

KW - vacuoles

KW - virulence

KW - MICROBIO

KW - CELLBIO

U2 - 10.1016/j.chom.2007.11.001

DO - 10.1016/j.chom.2007.11.001

M3 - Article

C2 - 18191792

VL - 3

SP - 30

EP - 38

JO - Cell Host & Microbe

JF - Cell Host & Microbe

SN - 1931-3128

IS - 1

ER -