Delta-crystallin enhancer binding protein delta EF1 is a zinc finger-homeodomain protein implicated in postgastrulation embryogenesis

J Funahashi, R Sekido, K Murai, Y Kamachi, H Kondoh

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

We investigated nuclear factors that bind to delta 1-crystallin enhancer core and regulate lens-specific transcription. A nuclear factor delta EF1, which binds to the essential element of the delta 1-crystallin enhancer core, was molecularly cloned from the chicken by a southwestern method. The protein organization of delta EF1 deduced from the cDNA sequence indicated that it has heterogeneous domains for DNA-binding, two widely separated zinc fingers and a homeodomain, analogous to Drosophila ZFH-1 protein. The C-terminal zinc fingers were found to be responsible for binding to the delta 1-crystallin enhancer core sequence. delta EF1 had proline-rich and acidic domains common to various transcriptional activators. During embryogenesis, delta EF1 expression was observed in the postgastrulation period in mesodermal tissues; initially, in the notochord, followed by somites, nephrotomes and other components. The expression level changed dynamically in a tissue, possibly reflecting the differentiation states of the constituent cells. Besides mesoderm, delta EF1 was expressed in the nervous system and the lens, but other ectodermal tissues and endoderm remained very low in delta EF1 expression. Cotransfection experiments indicated that this factor acts as a repressor of delta 1-crystallin enhancer. Possession of heterogeneous DNA-binding domains and its dynamic change of expression in embryogenesis strongly suggest that delta EF1 acts in multiple ways depending on the cell type and the gene under its regulation.

Original languageEnglish
Pages (from-to)433-46
Number of pages14
JournalDevelopment
Volume119
Issue number2
Publication statusPublished - Oct 1993

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delta-Crystallins
Homeodomain Proteins
Zinc Fingers
Embryonic Development
Carrier Proteins
Lenses
Notochord
Somites
Endoderm
DNA
Mesoderm
Proline
Nervous System
Drosophila
Chickens
Complementary DNA
Genes
Proteins

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain
  • Chick Embryo
  • DNA-Binding Proteins
  • Gene Expression
  • Genes, Homeobox
  • Genes, Regulator
  • Homeodomain Proteins
  • Humans
  • Immunohistochemistry
  • Lens, Crystalline
  • Mesoderm
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Morphogenesis
  • Nuclear Proteins
  • Sequence Homology
  • Transcription Factors
  • Zinc Fingers

Cite this

Delta-crystallin enhancer binding protein delta EF1 is a zinc finger-homeodomain protein implicated in postgastrulation embryogenesis. / Funahashi, J; Sekido, R; Murai, K; Kamachi, Y; Kondoh, H.

In: Development, Vol. 119, No. 2, 10.1993, p. 433-46.

Research output: Contribution to journalArticle

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AB - We investigated nuclear factors that bind to delta 1-crystallin enhancer core and regulate lens-specific transcription. A nuclear factor delta EF1, which binds to the essential element of the delta 1-crystallin enhancer core, was molecularly cloned from the chicken by a southwestern method. The protein organization of delta EF1 deduced from the cDNA sequence indicated that it has heterogeneous domains for DNA-binding, two widely separated zinc fingers and a homeodomain, analogous to Drosophila ZFH-1 protein. The C-terminal zinc fingers were found to be responsible for binding to the delta 1-crystallin enhancer core sequence. delta EF1 had proline-rich and acidic domains common to various transcriptional activators. During embryogenesis, delta EF1 expression was observed in the postgastrulation period in mesodermal tissues; initially, in the notochord, followed by somites, nephrotomes and other components. The expression level changed dynamically in a tissue, possibly reflecting the differentiation states of the constituent cells. Besides mesoderm, delta EF1 was expressed in the nervous system and the lens, but other ectodermal tissues and endoderm remained very low in delta EF1 expression. Cotransfection experiments indicated that this factor acts as a repressor of delta 1-crystallin enhancer. Possession of heterogeneous DNA-binding domains and its dynamic change of expression in embryogenesis strongly suggest that delta EF1 acts in multiple ways depending on the cell type and the gene under its regulation.

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