Demonstration of an immunosuppressive action of detergent-disrupted influenza virus on the antibody response to inactivated whole virus vaccine

R Jennings, R M Pemberton, T L Smith, T Amin, C W Potter

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In a series of experiments performed in hamsters and mice, administration of mixtures of detergent-disrupted (SV) influenza A X49 (H3N2) virus and inactivated X49 whole virus (WV) vaccine induced lower serum antibody titres than equivalent or lower doses of WV vaccine alone. This reduction in antibody titre was also observed using influenza A (H1N1) and influenza B (B/Hong Kong/8/73) SV and WV vaccine preparations. The results suggested that SV preparations can suppress the serum antibody response to WV vaccine. A suppressive effect of SV influenza virus on WV vaccine was also observed in an in vitro antibody-forming system, using primed mouse spleen cells. In this system, SV induced markedly lower IgG and IgM antibody responses than WV vaccine, and mixtures of SV with WV reproducibly resulted in lowered antibody responses compared to those elicited by WV alone. Possible reasons for these findings are discussed in the light of the known low immunogenicity observed for split and subunit influenza virus vaccine preparations in animals and in unprimed human populations.

Original languageEnglish
Pages (from-to)441-50
Number of pages10
JournalJournal of General Virology
Volume68 ( Pt 2)
DOIs
Publication statusPublished - Feb 1987

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Immunosuppressive Agents
Orthomyxoviridae
Detergents
Antibody Formation
Vaccines
Viruses
Human Influenza
Antibodies
H3N2 Subtype Influenza A Virus
Subunit Vaccines
Influenza Vaccines
Hong Kong
Serum
Cricetinae
Immunoglobulin M
Spleen
Immunoglobulin G
Population

Keywords

  • Animals
  • Antibodies, Viral
  • Cells, Cultured
  • Cricetinae
  • Detergents
  • Female
  • Immune Tolerance
  • Immunoglobulin G
  • Immunoglobulin M
  • Mesocricetus
  • Mice
  • Mice, Inbred BALB C
  • Orthomyxoviridae
  • Spleen
  • Surface-Active Agents
  • Vaccines, Attenuated
  • Viral Vaccines

Cite this

Demonstration of an immunosuppressive action of detergent-disrupted influenza virus on the antibody response to inactivated whole virus vaccine. / Jennings, R; Pemberton, R M; Smith, T L; Amin, T; Potter, C W.

In: Journal of General Virology, Vol. 68 ( Pt 2), 02.1987, p. 441-50.

Research output: Contribution to journalArticle

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abstract = "In a series of experiments performed in hamsters and mice, administration of mixtures of detergent-disrupted (SV) influenza A X49 (H3N2) virus and inactivated X49 whole virus (WV) vaccine induced lower serum antibody titres than equivalent or lower doses of WV vaccine alone. This reduction in antibody titre was also observed using influenza A (H1N1) and influenza B (B/Hong Kong/8/73) SV and WV vaccine preparations. The results suggested that SV preparations can suppress the serum antibody response to WV vaccine. A suppressive effect of SV influenza virus on WV vaccine was also observed in an in vitro antibody-forming system, using primed mouse spleen cells. In this system, SV induced markedly lower IgG and IgM antibody responses than WV vaccine, and mixtures of SV with WV reproducibly resulted in lowered antibody responses compared to those elicited by WV alone. Possible reasons for these findings are discussed in the light of the known low immunogenicity observed for split and subunit influenza virus vaccine preparations in animals and in unprimed human populations.",
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AB - In a series of experiments performed in hamsters and mice, administration of mixtures of detergent-disrupted (SV) influenza A X49 (H3N2) virus and inactivated X49 whole virus (WV) vaccine induced lower serum antibody titres than equivalent or lower doses of WV vaccine alone. This reduction in antibody titre was also observed using influenza A (H1N1) and influenza B (B/Hong Kong/8/73) SV and WV vaccine preparations. The results suggested that SV preparations can suppress the serum antibody response to WV vaccine. A suppressive effect of SV influenza virus on WV vaccine was also observed in an in vitro antibody-forming system, using primed mouse spleen cells. In this system, SV induced markedly lower IgG and IgM antibody responses than WV vaccine, and mixtures of SV with WV reproducibly resulted in lowered antibody responses compared to those elicited by WV alone. Possible reasons for these findings are discussed in the light of the known low immunogenicity observed for split and subunit influenza virus vaccine preparations in animals and in unprimed human populations.

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