Dependence on Dectin-1 Varies With Multiple Candida Species

Aiysha Thompson; James S Griffiths; Louise Walker; Diogo M da Fonseca; Keunsook K Lee; Philip R Taylor; Neil A R Gow; Selinda J Orr

doi:10.3389/fmicb.2019.01800

Dependence on Dectin-1 Varies With Multiple Candida Species

Aiysha Thompson, James S Griffiths, Louise Walker, Diogo M da Fonseca, Keunsook K Lee, Philip R Taylor, Neil A R Gow, Selinda J Orr (Corresponding Author)

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Abstract

Four Candida spp. (albicans, glabrata, tropicalis, parapsilosis) cause >95% of invasive Candida infections. C. albicans elicits immune responses via pathogen recognition receptors including C-type lectin-like receptors (CLRs). The CLR, Dectin-1 is important for host immunity to C. albicans and C. glabrata, however, whether Dectin-1 is important for host defense against C. tropicalis or C. parapsilosis is unknown. Therefore, we compared the involvement of Dectin-1 in response to these four diverse Candida spp. We found that Dectin-1 mediates innate cytokine responses to these Candida spp. in a species- and cell-dependent manner. Dectin-1 KO mice succumbed to infection with highly virulent C. albicans while they mostly survived infection with less virulent Candida spp. However, Dectin-1 KO mice displayed increased fungal burden following infection with each Candida spp. Additionally, T cells from Dectin-1 KO mice displayed enhanced effector functions likely due to the inability of Dectin-1 KO mice to clear the infections. Together, these data indicate that Dectin-1 is important for host defense to multiple Candida spp., although the specific roles for Dectin-1 varies with different Candida spp.

Original language	English
Article number	1800
Number of pages	12
Journal	Frontiers in Microbiology
Volume	10
DOIs	https://doi.org/10.3389/fmicb.2019.01800
Publication status	Published - 6 Aug 2019

Bibliographical note

FUNDING
SO was funded by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (Grant Number 099953/Z/12/Z) and by a Wellcome Trust Cross-Disciplinary
Award. PT is supported by a Wellcome Trust Investigator Award (107964/Z/15/Z) and the UK Dementia Research Institute. NG was supported by the Wellcome Trust Investigator, Collaborative, Equipment, Strategic and Biomedical Resource awards (086827, 075470, 097377, 101873, 200208, 093378, and 099197), and by the MRC Centre for Medical Mycology (N006364/1)

Keywords

Dectin-1
candida spp.
macrophages
dendritic cells
T cells
Candida spp
Dendritic cells
Macrophages

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10.3389/fmicb.2019.01800Licence: CC BY

Thom_DependenceOnDectin_VOR
Copyright © 2019 Thompson, Griffiths, Walker, da Fonseca, Lee, Taylor, Gow and Orr. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. https://creativecommons.org/licenses/by/4.0/
Final published version, 4.76 MBLicence: CC BY

Cite this

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title = "Dependence on Dectin-1 Varies With Multiple Candida Species",

abstract = "Four Candida spp. (albicans, glabrata, tropicalis, parapsilosis) cause >95% of invasive Candida infections. C. albicans elicits immune responses via pathogen recognition receptors including C-type lectin-like receptors (CLRs). The CLR, Dectin-1 is important for host immunity to C. albicans and C. glabrata, however, whether Dectin-1 is important for host defense against C. tropicalis or C. parapsilosis is unknown. Therefore, we compared the involvement of Dectin-1 in response to these four diverse Candida spp. We found that Dectin-1 mediates innate cytokine responses to these Candida spp. in a species- and cell-dependent manner. Dectin-1 KO mice succumbed to infection with highly virulent C. albicans while they mostly survived infection with less virulent Candida spp. However, Dectin-1 KO mice displayed increased fungal burden following infection with each Candida spp. Additionally, T cells from Dectin-1 KO mice displayed enhanced effector functions likely due to the inability of Dectin-1 KO mice to clear the infections. Together, these data indicate that Dectin-1 is important for host defense to multiple Candida spp., although the specific roles for Dectin-1 varies with different Candida spp.",

keywords = "Dectin-1, candida spp., macrophages, dendritic cells, T cells, Candida spp, Dendritic cells, Macrophages",

author = "Aiysha Thompson and Griffiths, {James S} and Louise Walker and {da Fonseca}, {Diogo M} and Lee, {Keunsook K} and Taylor, {Philip R} and Gow, {Neil A R} and Orr, {Selinda J}",

note = "FUNDING SO was funded by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (Grant Number 099953/Z/12/Z) and by a Wellcome Trust Cross-Disciplinary Award. PT is supported by a Wellcome Trust Investigator Award (107964/Z/15/Z) and the UK Dementia Research Institute. NG was supported by the Wellcome Trust Investigator, Collaborative, Equipment, Strategic and Biomedical Resource awards (086827, 075470, 097377, 101873, 200208, 093378, and 099197), and by the MRC Centre for Medical Mycology (N006364/1)",

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month = aug,

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doi = "10.3389/fmicb.2019.01800",

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AU - Thompson, Aiysha

AU - Griffiths, James S

AU - Walker, Louise

AU - da Fonseca, Diogo M

AU - Lee, Keunsook K

AU - Taylor, Philip R

AU - Gow, Neil A R

AU - Orr, Selinda J

N1 - FUNDING SO was funded by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (Grant Number 099953/Z/12/Z) and by a Wellcome Trust Cross-Disciplinary Award. PT is supported by a Wellcome Trust Investigator Award (107964/Z/15/Z) and the UK Dementia Research Institute. NG was supported by the Wellcome Trust Investigator, Collaborative, Equipment, Strategic and Biomedical Resource awards (086827, 075470, 097377, 101873, 200208, 093378, and 099197), and by the MRC Centre for Medical Mycology (N006364/1)

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N2 - Four Candida spp. (albicans, glabrata, tropicalis, parapsilosis) cause >95% of invasive Candida infections. C. albicans elicits immune responses via pathogen recognition receptors including C-type lectin-like receptors (CLRs). The CLR, Dectin-1 is important for host immunity to C. albicans and C. glabrata, however, whether Dectin-1 is important for host defense against C. tropicalis or C. parapsilosis is unknown. Therefore, we compared the involvement of Dectin-1 in response to these four diverse Candida spp. We found that Dectin-1 mediates innate cytokine responses to these Candida spp. in a species- and cell-dependent manner. Dectin-1 KO mice succumbed to infection with highly virulent C. albicans while they mostly survived infection with less virulent Candida spp. However, Dectin-1 KO mice displayed increased fungal burden following infection with each Candida spp. Additionally, T cells from Dectin-1 KO mice displayed enhanced effector functions likely due to the inability of Dectin-1 KO mice to clear the infections. Together, these data indicate that Dectin-1 is important for host defense to multiple Candida spp., although the specific roles for Dectin-1 varies with different Candida spp.

AB - Four Candida spp. (albicans, glabrata, tropicalis, parapsilosis) cause >95% of invasive Candida infections. C. albicans elicits immune responses via pathogen recognition receptors including C-type lectin-like receptors (CLRs). The CLR, Dectin-1 is important for host immunity to C. albicans and C. glabrata, however, whether Dectin-1 is important for host defense against C. tropicalis or C. parapsilosis is unknown. Therefore, we compared the involvement of Dectin-1 in response to these four diverse Candida spp. We found that Dectin-1 mediates innate cytokine responses to these Candida spp. in a species- and cell-dependent manner. Dectin-1 KO mice succumbed to infection with highly virulent C. albicans while they mostly survived infection with less virulent Candida spp. However, Dectin-1 KO mice displayed increased fungal burden following infection with each Candida spp. Additionally, T cells from Dectin-1 KO mice displayed enhanced effector functions likely due to the inability of Dectin-1 KO mice to clear the infections. Together, these data indicate that Dectin-1 is important for host defense to multiple Candida spp., although the specific roles for Dectin-1 varies with different Candida spp.

KW - Dectin-1

KW - candida spp.

KW - macrophages

KW - dendritic cells

KW - T cells

KW - Candida spp

KW - Dendritic cells

KW - Macrophages

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UR - http://www.mendeley.com/research/dependence-dectin1-varies-multiple-candida-species

U2 - 10.3389/fmicb.2019.01800

DO - 10.3389/fmicb.2019.01800

M3 - Article

C2 - 31447813

SN - 1664-302X

VL - 10

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

M1 - 1800

ER -

Dependence on Dectin-1 Varies With Multiple Candida Species

Abstract

Bibliographical note

Keywords

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