DETECTION OF A SINGLE GENETICALLY MODIFIED BACTERIAL-CELL IN SOIL BY USING CHARGE COUPLED DEVICE-ENHANCED MICROSCOPY

D J SILCOCK, R N WATERHOUSE, Lesley Anne Glover, James Ivor Prosser, Kenneth Stuart Killham

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Genes encoding bioluminescence from Vibrio harveyi were cloned into Pseudomonas syringae pv. phaseolicola, resulting in high levels of bioluminescence. After inoculation of sterile and nonsterile soil slurries with bioluminescent P. syringae, cells could not be identified by conventional light microscopy. However, when we used charge coupled device-enhanced microscopy, bioluminescent single cells were detected easily in dark fields despite masking by soil particulate matter, and in addition, the extent of competition from indigenous soil bacteria could be monitored. The technique which we describe offers great potential for tracking and determining the spatial distribution of genetically marked microorganisms in the environment.

Original languageEnglish
Pages (from-to)2444-2448
Number of pages5
JournalApplied and Environmental Microbiology
Volume58
Issue number8
Publication statusPublished - Aug 1992

Keywords

  • NUCLEATION-ACTIVE BACTERIA
  • ESCHERICHIA-COLI
  • BIOLOGICAL-CONTROL
  • VIBRIO-HARVEYI
  • FROST INJURY
  • EXPRESSION
  • PSEUDOMONAS
  • CONSTRUCTION
  • SELECTION
  • VECTORS

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