DETECTION OF A SINGLE GENETICALLY MODIFIED BACTERIAL-CELL IN SOIL BY USING CHARGE COUPLED DEVICE-ENHANCED MICROSCOPY

D J SILCOCK, R N WATERHOUSE, Lesley Anne Glover, James Ivor Prosser, Kenneth Stuart Killham

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Genes encoding bioluminescence from Vibrio harveyi were cloned into Pseudomonas syringae pv. phaseolicola, resulting in high levels of bioluminescence. After inoculation of sterile and nonsterile soil slurries with bioluminescent P. syringae, cells could not be identified by conventional light microscopy. However, when we used charge coupled device-enhanced microscopy, bioluminescent single cells were detected easily in dark fields despite masking by soil particulate matter, and in addition, the extent of competition from indigenous soil bacteria could be monitored. The technique which we describe offers great potential for tracking and determining the spatial distribution of genetically marked microorganisms in the environment.

Original languageEnglish
Pages (from-to)2444-2448
Number of pages5
JournalApplied and Environmental Microbiology
Volume58
Issue number8
Publication statusPublished - Aug 1992

Keywords

  • NUCLEATION-ACTIVE BACTERIA
  • ESCHERICHIA-COLI
  • BIOLOGICAL-CONTROL
  • VIBRIO-HARVEYI
  • FROST INJURY
  • EXPRESSION
  • PSEUDOMONAS
  • CONSTRUCTION
  • SELECTION
  • VECTORS

Cite this

DETECTION OF A SINGLE GENETICALLY MODIFIED BACTERIAL-CELL IN SOIL BY USING CHARGE COUPLED DEVICE-ENHANCED MICROSCOPY. / SILCOCK, D J ; WATERHOUSE, R N ; Glover, Lesley Anne; Prosser, James Ivor; Killham, Kenneth Stuart.

In: Applied and Environmental Microbiology, Vol. 58, No. 8, 08.1992, p. 2444-2448.

Research output: Contribution to journalArticle

SILCOCK, D J ; WATERHOUSE, R N ; Glover, Lesley Anne ; Prosser, James Ivor ; Killham, Kenneth Stuart. / DETECTION OF A SINGLE GENETICALLY MODIFIED BACTERIAL-CELL IN SOIL BY USING CHARGE COUPLED DEVICE-ENHANCED MICROSCOPY. In: Applied and Environmental Microbiology. 1992 ; Vol. 58, No. 8. pp. 2444-2448.
@article{65f7f622a2b24f22a67eda84ea546816,
title = "DETECTION OF A SINGLE GENETICALLY MODIFIED BACTERIAL-CELL IN SOIL BY USING CHARGE COUPLED DEVICE-ENHANCED MICROSCOPY",
abstract = "Genes encoding bioluminescence from Vibrio harveyi were cloned into Pseudomonas syringae pv. phaseolicola, resulting in high levels of bioluminescence. After inoculation of sterile and nonsterile soil slurries with bioluminescent P. syringae, cells could not be identified by conventional light microscopy. However, when we used charge coupled device-enhanced microscopy, bioluminescent single cells were detected easily in dark fields despite masking by soil particulate matter, and in addition, the extent of competition from indigenous soil bacteria could be monitored. The technique which we describe offers great potential for tracking and determining the spatial distribution of genetically marked microorganisms in the environment.",
keywords = "NUCLEATION-ACTIVE BACTERIA, ESCHERICHIA-COLI, BIOLOGICAL-CONTROL, VIBRIO-HARVEYI, FROST INJURY, EXPRESSION, PSEUDOMONAS, CONSTRUCTION, SELECTION, VECTORS",
author = "SILCOCK, {D J} and WATERHOUSE, {R N} and Glover, {Lesley Anne} and Prosser, {James Ivor} and Killham, {Kenneth Stuart}",
year = "1992",
month = "8",
language = "English",
volume = "58",
pages = "2444--2448",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - DETECTION OF A SINGLE GENETICALLY MODIFIED BACTERIAL-CELL IN SOIL BY USING CHARGE COUPLED DEVICE-ENHANCED MICROSCOPY

AU - SILCOCK, D J

AU - WATERHOUSE, R N

AU - Glover, Lesley Anne

AU - Prosser, James Ivor

AU - Killham, Kenneth Stuart

PY - 1992/8

Y1 - 1992/8

N2 - Genes encoding bioluminescence from Vibrio harveyi were cloned into Pseudomonas syringae pv. phaseolicola, resulting in high levels of bioluminescence. After inoculation of sterile and nonsterile soil slurries with bioluminescent P. syringae, cells could not be identified by conventional light microscopy. However, when we used charge coupled device-enhanced microscopy, bioluminescent single cells were detected easily in dark fields despite masking by soil particulate matter, and in addition, the extent of competition from indigenous soil bacteria could be monitored. The technique which we describe offers great potential for tracking and determining the spatial distribution of genetically marked microorganisms in the environment.

AB - Genes encoding bioluminescence from Vibrio harveyi were cloned into Pseudomonas syringae pv. phaseolicola, resulting in high levels of bioluminescence. After inoculation of sterile and nonsterile soil slurries with bioluminescent P. syringae, cells could not be identified by conventional light microscopy. However, when we used charge coupled device-enhanced microscopy, bioluminescent single cells were detected easily in dark fields despite masking by soil particulate matter, and in addition, the extent of competition from indigenous soil bacteria could be monitored. The technique which we describe offers great potential for tracking and determining the spatial distribution of genetically marked microorganisms in the environment.

KW - NUCLEATION-ACTIVE BACTERIA

KW - ESCHERICHIA-COLI

KW - BIOLOGICAL-CONTROL

KW - VIBRIO-HARVEYI

KW - FROST INJURY

KW - EXPRESSION

KW - PSEUDOMONAS

KW - CONSTRUCTION

KW - SELECTION

KW - VECTORS

M3 - Article

VL - 58

SP - 2444

EP - 2448

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 8

ER -