DETECTION OF HIGH-LEVELS OF GENETIC RELATEDNESS IN ROCK-POPULATIONS OF AN INTERTIDAL ISOPOD USING DNA-FINGERPRINTING

SB PIERTNEY, GR CARVALHO, Stuart Brannon Piertney

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6 Citations (Scopus)

Abstract

Multi-locus DNA fingerprinting was used to determine the degree of genetic relatedness within localized assemblages of the intertidal isopod, laera albifrons (Crustacea: Isopoda). This isopod has marsupial development, restricted adult vagility and specific habitat preferences, which will tend to isolate groups of animals on a shore and can potentially maintain the integrity of broods after release from the mother. Total genomic DNA was extracted from six individuals from each of ten 'rock-populations' in the South Wales area, restricted with RsaI, AlulII and HaeIII endonucleases, and probed using a (TCC), oligonucleotide probe. Band-sharing analysis of the fingerprint patterns indicated significantly higher genetic relatedness within rock-populations (mean within rock-population band-sharing value = 0.374 +/- 0.023 SE) than between (maximum between rock-population band-sharing value = 0.301 +/- 0.033) and at a level that equates to a relatedness between individuals within rock-populations of slightly below half-sibling status. The significance of such findings, in terms of the potential for localized inbreeding and population genetic structure, is discussed.

Original languageEnglish
Pages (from-to)967-976
Number of pages10
JournalJournal of the Marine Biological Association of the United Kingdom
Volume75
Issue number4
Publication statusPublished - Nov 1995

Keywords

  • PROSOBRANCHIA
  • REGIONS

Cite this

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title = "DETECTION OF HIGH-LEVELS OF GENETIC RELATEDNESS IN ROCK-POPULATIONS OF AN INTERTIDAL ISOPOD USING DNA-FINGERPRINTING",
abstract = "Multi-locus DNA fingerprinting was used to determine the degree of genetic relatedness within localized assemblages of the intertidal isopod, laera albifrons (Crustacea: Isopoda). This isopod has marsupial development, restricted adult vagility and specific habitat preferences, which will tend to isolate groups of animals on a shore and can potentially maintain the integrity of broods after release from the mother. Total genomic DNA was extracted from six individuals from each of ten 'rock-populations' in the South Wales area, restricted with RsaI, AlulII and HaeIII endonucleases, and probed using a (TCC), oligonucleotide probe. Band-sharing analysis of the fingerprint patterns indicated significantly higher genetic relatedness within rock-populations (mean within rock-population band-sharing value = 0.374 +/- 0.023 SE) than between (maximum between rock-population band-sharing value = 0.301 +/- 0.033) and at a level that equates to a relatedness between individuals within rock-populations of slightly below half-sibling status. The significance of such findings, in terms of the potential for localized inbreeding and population genetic structure, is discussed.",
keywords = "PROSOBRANCHIA, REGIONS",
author = "SB PIERTNEY and GR CARVALHO and Piertney, {Stuart Brannon}",
year = "1995",
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pages = "967--976",
journal = "Journal of the Marine Biological Association of the United Kingdom",
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TY - JOUR

T1 - DETECTION OF HIGH-LEVELS OF GENETIC RELATEDNESS IN ROCK-POPULATIONS OF AN INTERTIDAL ISOPOD USING DNA-FINGERPRINTING

AU - PIERTNEY, SB

AU - CARVALHO, GR

AU - Piertney, Stuart Brannon

PY - 1995/11

Y1 - 1995/11

N2 - Multi-locus DNA fingerprinting was used to determine the degree of genetic relatedness within localized assemblages of the intertidal isopod, laera albifrons (Crustacea: Isopoda). This isopod has marsupial development, restricted adult vagility and specific habitat preferences, which will tend to isolate groups of animals on a shore and can potentially maintain the integrity of broods after release from the mother. Total genomic DNA was extracted from six individuals from each of ten 'rock-populations' in the South Wales area, restricted with RsaI, AlulII and HaeIII endonucleases, and probed using a (TCC), oligonucleotide probe. Band-sharing analysis of the fingerprint patterns indicated significantly higher genetic relatedness within rock-populations (mean within rock-population band-sharing value = 0.374 +/- 0.023 SE) than between (maximum between rock-population band-sharing value = 0.301 +/- 0.033) and at a level that equates to a relatedness between individuals within rock-populations of slightly below half-sibling status. The significance of such findings, in terms of the potential for localized inbreeding and population genetic structure, is discussed.

AB - Multi-locus DNA fingerprinting was used to determine the degree of genetic relatedness within localized assemblages of the intertidal isopod, laera albifrons (Crustacea: Isopoda). This isopod has marsupial development, restricted adult vagility and specific habitat preferences, which will tend to isolate groups of animals on a shore and can potentially maintain the integrity of broods after release from the mother. Total genomic DNA was extracted from six individuals from each of ten 'rock-populations' in the South Wales area, restricted with RsaI, AlulII and HaeIII endonucleases, and probed using a (TCC), oligonucleotide probe. Band-sharing analysis of the fingerprint patterns indicated significantly higher genetic relatedness within rock-populations (mean within rock-population band-sharing value = 0.374 +/- 0.023 SE) than between (maximum between rock-population band-sharing value = 0.301 +/- 0.033) and at a level that equates to a relatedness between individuals within rock-populations of slightly below half-sibling status. The significance of such findings, in terms of the potential for localized inbreeding and population genetic structure, is discussed.

KW - PROSOBRANCHIA

KW - REGIONS

M3 - Article

VL - 75

SP - 967

EP - 976

JO - Journal of the Marine Biological Association of the United Kingdom

JF - Journal of the Marine Biological Association of the United Kingdom

SN - 0025-3154

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ER -