Detection of simian immunodeficiency virus RNA from infected rhesus macaques by the polymerase chain reaction

David J. Blackbourn, Linda F. Chuang, Suganto Sutjipto, Keith F. Killam, Paula M. McCready, Roy H. Doi, Yen Li, Ronald Y. Chuang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

A rapid method for the detection of simian immunodeficiency virus (SIV) RNA from peripheral blood mononuclear cells (PBMC) of experimentally infected rhesus macaques by the polymerase chain reaction (PCR) is reported. The PCR was carried out with a complementary DNA (cDNA) template using 3 pairs of primers that were designed to anneal to homologous sequences in conserved regions of 3 molecular clones of SIVmac. The specificity of the primers was confirmed by performing the PCR with template DNA from the 3 molecular clones. SIV-specific RNA was detected from 30 and 50 infected PBMC/6.25 × 105 PBMC of two animals.

Original languageEnglish
Pages (from-to)109-117
Number of pages9
JournalJournal of Virological Methods
Volume37
Issue number2
DOIs
Publication statusPublished - May 1992

Keywords

  • PCR
  • RNA
  • SIV

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