Detection of simian immunodeficiency virus RNA from infected rhesus macaques by the polymerase chain reaction

David J. Blackbourn; Linda F. Chuang; Suganto Sutjipto; Keith F. Killam; Paula M. McCready; Roy H. Doi; Yen Li; Ronald Y. Chuang

doi:10.1016/0166-0934(92)90038-F

Detection of simian immunodeficiency virus RNA from infected rhesus macaques by the polymerase chain reaction

David J. Blackbourn, Linda F. Chuang, Suganto Sutjipto, Keith F. Killam, Paula M. McCready, Roy H. Doi, Yen Li, Ronald Y. Chuang^*

^*Corresponding author for this work

Medical Sciences

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

A rapid method for the detection of simian immunodeficiency virus (SIV) RNA from peripheral blood mononuclear cells (PBMC) of experimentally infected rhesus macaques by the polymerase chain reaction (PCR) is reported. The PCR was carried out with a complementary DNA (cDNA) template using 3 pairs of primers that were designed to anneal to homologous sequences in conserved regions of 3 molecular clones of SIV_mac. The specificity of the primers was confirmed by performing the PCR with template DNA from the 3 molecular clones. SIV-specific RNA was detected from 30 and 50 infected PBMC/6.25 × 10⁵ PBMC of two animals.

Original language	English
Pages (from-to)	109-117
Number of pages	9
Journal	Journal of Virological Methods
Volume	37
Issue number	2
DOIs	https://doi.org/10.1016/0166-0934(92)90038-F
Publication status	Published - May 1992

Keywords

PCR
RNA
SIV

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10.1016/0166-0934(92)90038-F

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title = "Detection of simian immunodeficiency virus RNA from infected rhesus macaques by the polymerase chain reaction",

abstract = "A rapid method for the detection of simian immunodeficiency virus (SIV) RNA from peripheral blood mononuclear cells (PBMC) of experimentally infected rhesus macaques by the polymerase chain reaction (PCR) is reported. The PCR was carried out with a complementary DNA (cDNA) template using 3 pairs of primers that were designed to anneal to homologous sequences in conserved regions of 3 molecular clones of SIVmac. The specificity of the primers was confirmed by performing the PCR with template DNA from the 3 molecular clones. SIV-specific RNA was detected from 30 and 50 infected PBMC/6.25 × 105 PBMC of two animals.",

keywords = "PCR, RNA, SIV",

author = "Blackbourn, {David J.} and Chuang, {Linda F.} and Suganto Sutjipto and Killam, {Keith F.} and McCready, {Paula M.} and Doi, {Roy H.} and Yen Li and Chuang, {Ronald Y.}",

note = "Funding Information: This researchw ass upportedb y NIDA researchg rantD A-05901a nd Hawaii HeptachlorR esearcha nd EducationF oundation grant HHHERP 88-02.",

year = "1992",

month = may,

doi = "10.1016/0166-0934(92)90038-F",

language = "English",

volume = "37",

pages = "109--117",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier",

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TY - JOUR

T1 - Detection of simian immunodeficiency virus RNA from infected rhesus macaques by the polymerase chain reaction

AU - Blackbourn, David J.

AU - Chuang, Linda F.

AU - Sutjipto, Suganto

AU - Killam, Keith F.

AU - McCready, Paula M.

AU - Doi, Roy H.

AU - Li, Yen

AU - Chuang, Ronald Y.

N1 - Funding Information: This researchw ass upportedb y NIDA researchg rantD A-05901a nd Hawaii HeptachlorR esearcha nd EducationF oundation grant HHHERP 88-02.

PY - 1992/5

Y1 - 1992/5

N2 - A rapid method for the detection of simian immunodeficiency virus (SIV) RNA from peripheral blood mononuclear cells (PBMC) of experimentally infected rhesus macaques by the polymerase chain reaction (PCR) is reported. The PCR was carried out with a complementary DNA (cDNA) template using 3 pairs of primers that were designed to anneal to homologous sequences in conserved regions of 3 molecular clones of SIVmac. The specificity of the primers was confirmed by performing the PCR with template DNA from the 3 molecular clones. SIV-specific RNA was detected from 30 and 50 infected PBMC/6.25 × 105 PBMC of two animals.

AB - A rapid method for the detection of simian immunodeficiency virus (SIV) RNA from peripheral blood mononuclear cells (PBMC) of experimentally infected rhesus macaques by the polymerase chain reaction (PCR) is reported. The PCR was carried out with a complementary DNA (cDNA) template using 3 pairs of primers that were designed to anneal to homologous sequences in conserved regions of 3 molecular clones of SIVmac. The specificity of the primers was confirmed by performing the PCR with template DNA from the 3 molecular clones. SIV-specific RNA was detected from 30 and 50 infected PBMC/6.25 × 105 PBMC of two animals.

KW - PCR

KW - RNA

KW - SIV

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U2 - 10.1016/0166-0934(92)90038-F

DO - 10.1016/0166-0934(92)90038-F

M3 - Article

C2 - 1597502

AN - SCOPUS:0026608078

VL - 37

SP - 109

EP - 117

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

IS - 2

ER -

Detection of simian immunodeficiency virus RNA from infected rhesus macaques by the polymerase chain reaction

Abstract

Keywords

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