Determination of 3,4-dihydroxyphenylglycol, hydroxytyrosol and tyrosol purified from olive oil by-products with HPLC in animal plasma and tissues

Guillermo Rodriguez-Gutierrez, Sharon Wood, Juan Fernandez-Bolanos Guzman, Garry G. Duthie, Baukje de Roos

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A simple, fast and reliable method to quantify, simultaneously, 3,4-dihydroxyphenylglycol (DHPG), hydroxytyrosol (HT) and tyrosol (Ty) extracted and purified successfully from olive oil by-product, called alperujo, in animal plasma and tissues samples has been developed using a high-performance liquid chromatographic (HPLC) method with UV-Vis detection. Extraction of compounds is based on solid-phase extraction for plasma and homogenisation with zirconia beads and centrifugation for tissues. Calibration curves were linear for all three phenols at a relatively low concentration range (0.05-50 mu g/mL). This method has acceptable accuracy (91-95% in plasma and 63-100% in tissues), precision (1.11-8.26% intra-day and 0.32-9.5% inter-day) and sensitivity for detecting low concentrations of these phenols in small plasma volumes and several animal tissues such as liver, heart, kidney, muscle, testes, white adipose tissue (WAT) and brain. (c) 2010 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1948-1952
Number of pages5
JournalFood Chemistry
Issue number4
Publication statusPublished - 15 Jun 2011


  • olive oil
  • alperujo
  • 3,4-dihydroxyphenylglycol
  • hydroxytyrosol
  • tyrosol
  • anti-oxidant
  • HPLC
  • animal tissues
  • animal plasma
  • performance liquid-chromatography
  • fluorescence detection
  • antioxidant activity
  • polyphenols
  • oleuropein
  • compound
  • glycol
  • waste
  • brain
  • blood

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