Abstract
The amino acid fraction from rat liver, heart and skeletal muscle was prepared by the separation of sulphosalicylic acid extract on Dowex 50 H+ form. The presence of L-methionine-dl-sulphoxide in these extracts was identified and compared by three independent chromatographic methods: ion-exchange, Pico-Tag and reversed-phase high-performance liquid chromatography after precolumn derivatisation with diethylethoxymethylenemalonate. Quantitative data indicate that L-methionine-dl-sulphoxide is present in the intracellular pool at the levels of free methionine.
Original language | English |
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Pages (from-to) | 299-305 |
Number of pages | 7 |
Journal | Journal of Chromatography A |
Volume | 619 |
Issue number | 2 |
Publication status | Published - 22 Sept 1993 |
Keywords
- Animals
- Chromatography, High Pressure Liquid
- Chromatography, Ion Exchange
- Electrochemistry
- Indicators and Reagents
- Liver Extracts
- Male
- Methionine
- Muscles
- Myocardium
- Rats
- Spectrophotometry, Ultraviolet
- Stereoisomerism
- Tissue Extracts