Determination of L-methionine-dl-sulphoxide in tissue extracts

P W Scislowski, I. Leon Harris, K Pickard, David Stanley Brown, Vivien Buchan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The amino acid fraction from rat liver, heart and skeletal muscle was prepared by the separation of sulphosalicylic acid extract on Dowex 50 H+ form. The presence of L-methionine-dl-sulphoxide in these extracts was identified and compared by three independent chromatographic methods: ion-exchange, Pico-Tag and reversed-phase high-performance liquid chromatography after precolumn derivatisation with diethylethoxymethylenemalonate. Quantitative data indicate that L-methionine-dl-sulphoxide is present in the intracellular pool at the levels of free methionine.
Original languageEnglish
Pages (from-to)299-305
Number of pages7
JournalJournal of Chromatography A
Volume619
Issue number2
Publication statusPublished - 22 Sep 1993

Keywords

  • Animals
  • Chromatography, High Pressure Liquid
  • Chromatography, Ion Exchange
  • Electrochemistry
  • Indicators and Reagents
  • Liver Extracts
  • Male
  • Methionine
  • Muscles
  • Myocardium
  • Rats
  • Spectrophotometry, Ultraviolet
  • Stereoisomerism
  • Tissue Extracts

Fingerprint Dive into the research topics of 'Determination of L-methionine-dl-sulphoxide in tissue extracts'. Together they form a unique fingerprint.

Cite this