Determination of L-methionine-dl-sulphoxide in tissue extracts

P W Scislowski, I. Leon Harris, K Pickard, David Stanley Brown, Vivien Buchan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The amino acid fraction from rat liver, heart and skeletal muscle was prepared by the separation of sulphosalicylic acid extract on Dowex 50 H+ form. The presence of L-methionine-dl-sulphoxide in these extracts was identified and compared by three independent chromatographic methods: ion-exchange, Pico-Tag and reversed-phase high-performance liquid chromatography after precolumn derivatisation with diethylethoxymethylenemalonate. Quantitative data indicate that L-methionine-dl-sulphoxide is present in the intracellular pool at the levels of free methionine.
Original languageEnglish
Pages (from-to)299-305
Number of pages7
JournalJournal of Chromatography A
Volume619
Issue number2
Publication statusPublished - 22 Sep 1993

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Tissue Extracts
Methionine
Ion Exchange
High performance liquid chromatography
Reverse-Phase Chromatography
Liver
Muscle
Rats
Ion exchange
Myocardium
Skeletal Muscle
High Pressure Liquid Chromatography
Amino Acids
Acids
methionine sulfoxide

Keywords

  • Animals
  • Chromatography, High Pressure Liquid
  • Chromatography, Ion Exchange
  • Electrochemistry
  • Indicators and Reagents
  • Liver Extracts
  • Male
  • Methionine
  • Muscles
  • Myocardium
  • Rats
  • Spectrophotometry, Ultraviolet
  • Stereoisomerism
  • Tissue Extracts

Cite this

Scislowski, P. W., Harris, I. L., Pickard, K., Brown, D. S., & Buchan, V. (1993). Determination of L-methionine-dl-sulphoxide in tissue extracts. Journal of Chromatography A, 619(2), 299-305.

Determination of L-methionine-dl-sulphoxide in tissue extracts. / Scislowski, P W; Harris, I. Leon; Pickard, K; Brown, David Stanley; Buchan, Vivien.

In: Journal of Chromatography A, Vol. 619, No. 2, 22.09.1993, p. 299-305.

Research output: Contribution to journalArticle

Scislowski, PW, Harris, IL, Pickard, K, Brown, DS & Buchan, V 1993, 'Determination of L-methionine-dl-sulphoxide in tissue extracts', Journal of Chromatography A, vol. 619, no. 2, pp. 299-305.
Scislowski PW, Harris IL, Pickard K, Brown DS, Buchan V. Determination of L-methionine-dl-sulphoxide in tissue extracts. Journal of Chromatography A. 1993 Sep 22;619(2):299-305.
Scislowski, P W ; Harris, I. Leon ; Pickard, K ; Brown, David Stanley ; Buchan, Vivien. / Determination of L-methionine-dl-sulphoxide in tissue extracts. In: Journal of Chromatography A. 1993 ; Vol. 619, No. 2. pp. 299-305.
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