Determining SNP allele frequencies in DNA pools

G Breen, D Harold, S Ralston, D Shaw, D S Clair

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Single nucleotide polymorphisms (SNPs) are among the most common types of polymorphism used for genetic association studies. A method to allow the accurate quantitation of their allele frequencies from DNA pools would both increase throughput and decrease costs for large-scale genotyping. However to date, most DNA pooling studies have concentrated on the use of microsatellite polymorphisms. In the case of SNPs that are restriction fragment length polymorphisms (RFLPs), studies have tended to use methods for the quantitation of allele frequency from pools that rely on densitometric evaluation of bands on an autoradiograph. Radiation-based methods have well-known drawbacks, and we pre sent two alternate methods for the detennination of SNP allele frequencies. For RFLPs, we used agarose gel electrophoresis of digested PCR products with ethidium bromide staining combined with densitometric analysis of gel images on a PC. For all types of SNP, we used allele-specific flu orescent probes in the Taqman(R) assay to determine the relative frequencies of of different alleles. Both methods gave accurate and reproducible results, suggesting they are suitable for use in DNA pooling experiments.

Original languageEnglish
Pages (from-to)464
Number of pages5
JournalBiotechniques
Volume28
Publication statusPublished - 2000

Keywords

  • GENE

Cite this

Breen, G., Harold, D., Ralston, S., Shaw, D., & Clair, D. S. (2000). Determining SNP allele frequencies in DNA pools. Biotechniques, 28, 464.

Determining SNP allele frequencies in DNA pools. / Breen, G ; Harold, D ; Ralston, S ; Shaw, D ; Clair, D S .

In: Biotechniques, Vol. 28, 2000, p. 464.

Research output: Contribution to journalArticle

Breen, G, Harold, D, Ralston, S, Shaw, D & Clair, DS 2000, 'Determining SNP allele frequencies in DNA pools', Biotechniques, vol. 28, pp. 464.
Breen G, Harold D, Ralston S, Shaw D, Clair DS. Determining SNP allele frequencies in DNA pools. Biotechniques. 2000;28:464.
Breen, G ; Harold, D ; Ralston, S ; Shaw, D ; Clair, D S . / Determining SNP allele frequencies in DNA pools. In: Biotechniques. 2000 ; Vol. 28. pp. 464.
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