Development and application of bioluminescent Caenorhabditis elegans as multicellular eukaryotic biosensors

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Abstract

We describe a novel approach to assess toxicity to the free-living nematode Caenorhabditis elegans that relies on the ability of firefly luciferase to report on endogenous ATP levels. We have constructed bioluminescent C, elegans with the luc gene under control of a constitutive promoter, Light reduction was observed in response to increasing temperature, concentrations of copper, lead and 3,5-dichlorophenol, This was due to increased mortality coupled with decreased metabolic activity in the surviving animals. The light emitted by the transgenic nematodes gave a rapid, real-time indication of metabolic status. This forms the basis of rapid and biologically relevant toxicity tests, (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)36-39
Number of pages3
JournalFEBS Letters
Volume493
Issue number1
Early online date7 Mar 2001
DOIs
Publication statusPublished - 23 Mar 2001

Keywords

  • firefly luciferase
  • bioluminescence
  • eukaryotic biosensor
  • toxicology
  • ATP
  • caenorhabditis elegans
  • soil toxicity test
  • ion characteristics
  • metal-ions
  • nematode
  • gene
  • stress
  • expression
  • strains
  • LC50

Cite this

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title = "Development and application of bioluminescent Caenorhabditis elegans as multicellular eukaryotic biosensors",
abstract = "We describe a novel approach to assess toxicity to the free-living nematode Caenorhabditis elegans that relies on the ability of firefly luciferase to report on endogenous ATP levels. We have constructed bioluminescent C, elegans with the luc gene under control of a constitutive promoter, Light reduction was observed in response to increasing temperature, concentrations of copper, lead and 3,5-dichlorophenol, This was due to increased mortality coupled with decreased metabolic activity in the surviving animals. The light emitted by the transgenic nematodes gave a rapid, real-time indication of metabolic status. This forms the basis of rapid and biologically relevant toxicity tests, (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.",
keywords = "firefly luciferase, bioluminescence, eukaryotic biosensor, toxicology, ATP, caenorhabditis elegans, soil toxicity test, ion characteristics, metal-ions, nematode, gene, stress, expression, strains, LC50",
author = "Cristina Lagido and Jonathan Pettitt and Porter, {Andrew Justin Radcliffe} and Paton, {Graeme Iain} and Glover, {Lesley Anne}",
year = "2001",
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doi = "10.1016/S0014-5793(01)02271-2",
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journal = "FEBS Letters",
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TY - JOUR

T1 - Development and application of bioluminescent Caenorhabditis elegans as multicellular eukaryotic biosensors

AU - Lagido, Cristina

AU - Pettitt, Jonathan

AU - Porter, Andrew Justin Radcliffe

AU - Paton, Graeme Iain

AU - Glover, Lesley Anne

PY - 2001/3/23

Y1 - 2001/3/23

N2 - We describe a novel approach to assess toxicity to the free-living nematode Caenorhabditis elegans that relies on the ability of firefly luciferase to report on endogenous ATP levels. We have constructed bioluminescent C, elegans with the luc gene under control of a constitutive promoter, Light reduction was observed in response to increasing temperature, concentrations of copper, lead and 3,5-dichlorophenol, This was due to increased mortality coupled with decreased metabolic activity in the surviving animals. The light emitted by the transgenic nematodes gave a rapid, real-time indication of metabolic status. This forms the basis of rapid and biologically relevant toxicity tests, (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

AB - We describe a novel approach to assess toxicity to the free-living nematode Caenorhabditis elegans that relies on the ability of firefly luciferase to report on endogenous ATP levels. We have constructed bioluminescent C, elegans with the luc gene under control of a constitutive promoter, Light reduction was observed in response to increasing temperature, concentrations of copper, lead and 3,5-dichlorophenol, This was due to increased mortality coupled with decreased metabolic activity in the surviving animals. The light emitted by the transgenic nematodes gave a rapid, real-time indication of metabolic status. This forms the basis of rapid and biologically relevant toxicity tests, (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

KW - firefly luciferase

KW - bioluminescence

KW - eukaryotic biosensor

KW - toxicology

KW - ATP

KW - caenorhabditis elegans

KW - soil toxicity test

KW - ion characteristics

KW - metal-ions

KW - nematode

KW - gene

KW - stress

KW - expression

KW - strains

KW - LC50

U2 - 10.1016/S0014-5793(01)02271-2

DO - 10.1016/S0014-5793(01)02271-2

M3 - Article

VL - 493

SP - 36

EP - 39

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -