Development and Characterization of Somatic Hybrids of Ulva reticulata Forsskål (×) Monostroma oxyspermum (Kutz.)Doty

Vishal Gupta; Puja Kumari; C. R.K. Reddy

doi:10.3389/fpls.2015.00003

Development and Characterization of Somatic Hybrids of Ulva reticulata Forsskål (×) Monostroma oxyspermum (Kutz.)Doty

Vishal Gupta, Puja Kumari, C. R.K. Reddy^*

^*Corresponding author for this work

Aberdeen Centre For Environmental Sustainability

CSIR - Indian Institute of Chemical Technology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Ulvophycean species with diverse trait characteristics provide an opportunity to create novel allelic recombinant variants. The present study reports the development of seaweed variants with improved agronomic traits through protoplast fusion between Monostroma oxyspermum (Kutz.) Doty and Ulva reticulata Forsskal. A total of 12 putative hybrids were screened based on the variations in morphology and total DNA content over the fusion partners. DNA-fingerprinting by inter simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP) analysis confirmed genomic introgression in the hybrids. The DNA fingerprint revealed sharing of parental alleles in regenerated hybrids and a few alleles that were unique to hybrids. The epigenetic variations in hybrids estimated in terms of DNA methylation polymorphism also revealed sharing of methylation loci with both the fusion partners. The functional trait analysis for growth showed a hybrid with heterotic trait (DGR% = 36.7 ± 1.55%) over the fusion partners U. reticulata (33.2 ± 2.6%) and M. oxyspermum (17.8 ± 1.77%), while others were superior to the mid-parental value (25.2 ± 2.2%) (p < 0.05). The fatty acid (FA) analysis of hybrids showed notable variations over fusion partners. Most hybrids showed increased polyunsaturated FAs (PUFAs) compared to saturated FAs (SFAs) and mainly includes the nutritionally important linoleic acid, a-linolenic acid, oleic acid, stearidonic acid, and docosahexaenoic acid. The other differences observed include superior cellulose content and antioxidative potential in hybrids over fusion partners. The hybrid varieties with superior traits developed in this study unequivocally demonstrate the significance of protoplast fusion technique in developing improved varients of macroalgae.

Original language	English
Article number	3
Pages (from-to)	1-15
Number of pages	15
Journal	Frontiers in plant science
Volume	6
Issue number	JAN
DOIs	https://doi.org/10.3389/fpls.2015.00003
Publication status	Published - 29 Jan 2015

Bibliographical note

Publisher Copyright:
© 2015 Gupta, Kumari and Reddy.

Acknowledgments: The financial support received from DBT Indo-UK collaborative project (SuBBSea) under grant agreement no. BT/IN/Indo-UK/SuBB/22/AML/2013 is gratefully acknowledged. Dr. Juliet C. Coates, Birmingham University, UK is acknowledged for English corrections. We are thankful to the anonymous reviewers for their valuable comments.

Data Availability Statement

Supplementary Material for this article can be found online at: http://www.frontiersin.org/journal/10.3389/fpls.2015.00003/abstract

Keywords

Heterosis
Hybrid
Monostroma
Protoplast
Ulva

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title = "Development and Characterization of Somatic Hybrids of Ulva reticulata Forssk{\aa}l (×) Monostroma oxyspermum (Kutz.)Doty",

abstract = "Ulvophycean species with diverse trait characteristics provide an opportunity to create novel allelic recombinant variants. The present study reports the development of seaweed variants with improved agronomic traits through protoplast fusion between Monostroma oxyspermum (Kutz.) Doty and Ulva reticulata Forsskal. A total of 12 putative hybrids were screened based on the variations in morphology and total DNA content over the fusion partners. DNA-fingerprinting by inter simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP) analysis confirmed genomic introgression in the hybrids. The DNA fingerprint revealed sharing of parental alleles in regenerated hybrids and a few alleles that were unique to hybrids. The epigenetic variations in hybrids estimated in terms of DNA methylation polymorphism also revealed sharing of methylation loci with both the fusion partners. The functional trait analysis for growth showed a hybrid with heterotic trait (DGR% = 36.7 ± 1.55%) over the fusion partners U. reticulata (33.2 ± 2.6%) and M. oxyspermum (17.8 ± 1.77%), while others were superior to the mid-parental value (25.2 ± 2.2%) (p < 0.05). The fatty acid (FA) analysis of hybrids showed notable variations over fusion partners. Most hybrids showed increased polyunsaturated FAs (PUFAs) compared to saturated FAs (SFAs) and mainly includes the nutritionally important linoleic acid, a-linolenic acid, oleic acid, stearidonic acid, and docosahexaenoic acid. The other differences observed include superior cellulose content and antioxidative potential in hybrids over fusion partners. The hybrid varieties with superior traits developed in this study unequivocally demonstrate the significance of protoplast fusion technique in developing improved varients of macroalgae.",

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Development and Characterization of Somatic Hybrids of Ulva reticulata Forsskål (×) Monostroma oxyspermum (Kutz.)Doty

Abstract

Bibliographical note

Data Availability Statement

Keywords

Access to Document

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