Development of a fast screening method for the direct determination of chlorinated persistent organic pollutants in fish oil by high-resolution continuum source graphite furnace molecular absorption spectrometry

Éderson R Pereira, Josias Merib, Heloisa R. Cadorim, Mauana Schneider, Gabriel S. Carvalho, Fabio A. Duarte, Bernhard Welz (Corresponding Author), Javier Del Campo Menoyo, Jorg Feldmann

Research output: Contribution to journalArticle

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Abstract

The occurrence of persistent organic pollutants (POPs), mainly organochlorine pesticides and polychlorinated biphenyls, was directly
associated with several diseases and environmental endocrine disrupting. In the aquatic environment, POPs can accumulate in fish lipoid tissues due to their high hydrophobicity, and become this way one of the main sources of human exposure to POPs through the consumption of fish meat and oil as Omega-3 source. Chlorine might serve as a proxy for the presence of POPs, and a fast screening of chlorine in a complex matrix, such as fish oil, could provide substantial information about the contamination with POPs. Therefore, a method has been developed in this work for the determination of total chlorine in fish oil samples via molecular absorption of the strontium mono-chloride molecule in the gas phase using high-resolution continuum source graphite furnace molecular absorption spectrometry. The effect of zirconium as permanent chemical modifier in the pyrolysis and vaporization stages was optimized in order to avoid the need for any kind of sample preparation prior to the determination of total chlorine, using just a dilution with 1-propanol. The accuracy has been evaluated using micro-coulometric titration after sample combustion, and the values were statistically in agreement (95% confidence level) between both techniques. The method has been applied for the determination of total chlorine in five different fractions of a commercial pooled marine fish oil sample collected from the Pacific
Ocean, where the majority of the fish is Peruvian anchovy (Engraulis ringens), two commercial oils from Brazil and three Omega-3 supplements acquired in Germany. The limit of detection of the procedure is 1.8 ng Cl absolute or 0.9 µg g-1 Cl in the fish oil. The time required for a single determination is less than 5 min, and less than 15 min for a triplicate determination.
Original languageEnglish
Pages (from-to)456-462
Number of pages7
JournalFood control
Volume78
Early online date19 Mar 2017
DOIs
Publication statusPublished - Aug 2017

Fingerprint

persistent organic pollutants
furnaces
Graphite
Fish Oils
Chlorine
chlorine
fish oils
spectroscopy
Spectrum Analysis
screening
Fishes
Industrial Oils
1-propanol
Endocrine System Diseases
sampling
1-Propanol
fish meat
methodology
Volatilization
organochlorine pesticides

Keywords

  • persistent organic pollutants
  • organochlorines
  • total chlorine determination
  • fish oil
  • Omega-3 supplements
  • high-resolution continuum source molecular 45 absorption spectrometry

Cite this

Development of a fast screening method for the direct determination of chlorinated persistent organic pollutants in fish oil by high-resolution continuum source graphite furnace molecular absorption spectrometry. / Pereira, Éderson R; Merib, Josias ; Cadorim, Heloisa R. ; Schneider, Mauana ; Carvalho, Gabriel S. ; Duarte, Fabio A.; Welz, Bernhard (Corresponding Author); Campo Menoyo, Javier Del; Feldmann, Jorg.

In: Food control, Vol. 78, 08.2017, p. 456-462.

Research output: Contribution to journalArticle

Pereira, Éderson R ; Merib, Josias ; Cadorim, Heloisa R. ; Schneider, Mauana ; Carvalho, Gabriel S. ; Duarte, Fabio A. ; Welz, Bernhard ; Campo Menoyo, Javier Del ; Feldmann, Jorg. / Development of a fast screening method for the direct determination of chlorinated persistent organic pollutants in fish oil by high-resolution continuum source graphite furnace molecular absorption spectrometry. In: Food control. 2017 ; Vol. 78. pp. 456-462.
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abstract = "The occurrence of persistent organic pollutants (POPs), mainly organochlorine pesticides and polychlorinated biphenyls, was directlyassociated with several diseases and environmental endocrine disrupting. In the aquatic environment, POPs can accumulate in fish lipoid tissues due to their high hydrophobicity, and become this way one of the main sources of human exposure to POPs through the consumption of fish meat and oil as Omega-3 source. Chlorine might serve as a proxy for the presence of POPs, and a fast screening of chlorine in a complex matrix, such as fish oil, could provide substantial information about the contamination with POPs. Therefore, a method has been developed in this work for the determination of total chlorine in fish oil samples via molecular absorption of the strontium mono-chloride molecule in the gas phase using high-resolution continuum source graphite furnace molecular absorption spectrometry. The effect of zirconium as permanent chemical modifier in the pyrolysis and vaporization stages was optimized in order to avoid the need for any kind of sample preparation prior to the determination of total chlorine, using just a dilution with 1-propanol. The accuracy has been evaluated using micro-coulometric titration after sample combustion, and the values were statistically in agreement (95{\%} confidence level) between both techniques. The method has been applied for the determination of total chlorine in five different fractions of a commercial pooled marine fish oil sample collected from the PacificOcean, where the majority of the fish is Peruvian anchovy (Engraulis ringens), two commercial oils from Brazil and three Omega-3 supplements acquired in Germany. The limit of detection of the procedure is 1.8 ng Cl absolute or 0.9 µg g-1 Cl in the fish oil. The time required for a single determination is less than 5 min, and less than 15 min for a triplicate determination.",
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T1 - Development of a fast screening method for the direct determination of chlorinated persistent organic pollutants in fish oil by high-resolution continuum source graphite furnace molecular absorption spectrometry

AU - Pereira, Éderson R

AU - Merib, Josias

AU - Cadorim, Heloisa R.

AU - Schneider, Mauana

AU - Carvalho, Gabriel S.

AU - Duarte, Fabio A.

AU - Welz, Bernhard

AU - Campo Menoyo, Javier Del

AU - Feldmann, Jorg

N1 - The authors are grateful to the Conselho Nacional de Desenvolvimento Científico and Tecnológico (CNPq); the present research was mostly financed through Project no. CNPq 406877/2013-0. The authors are also grateful to the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for financial support and scholarships, and to Analytik Jena for financial support and donation of the contrAA 600 high-resolution continuum source atomic absorption spectrometer.

PY - 2017/8

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N2 - The occurrence of persistent organic pollutants (POPs), mainly organochlorine pesticides and polychlorinated biphenyls, was directlyassociated with several diseases and environmental endocrine disrupting. In the aquatic environment, POPs can accumulate in fish lipoid tissues due to their high hydrophobicity, and become this way one of the main sources of human exposure to POPs through the consumption of fish meat and oil as Omega-3 source. Chlorine might serve as a proxy for the presence of POPs, and a fast screening of chlorine in a complex matrix, such as fish oil, could provide substantial information about the contamination with POPs. Therefore, a method has been developed in this work for the determination of total chlorine in fish oil samples via molecular absorption of the strontium mono-chloride molecule in the gas phase using high-resolution continuum source graphite furnace molecular absorption spectrometry. The effect of zirconium as permanent chemical modifier in the pyrolysis and vaporization stages was optimized in order to avoid the need for any kind of sample preparation prior to the determination of total chlorine, using just a dilution with 1-propanol. The accuracy has been evaluated using micro-coulometric titration after sample combustion, and the values were statistically in agreement (95% confidence level) between both techniques. The method has been applied for the determination of total chlorine in five different fractions of a commercial pooled marine fish oil sample collected from the PacificOcean, where the majority of the fish is Peruvian anchovy (Engraulis ringens), two commercial oils from Brazil and three Omega-3 supplements acquired in Germany. The limit of detection of the procedure is 1.8 ng Cl absolute or 0.9 µg g-1 Cl in the fish oil. The time required for a single determination is less than 5 min, and less than 15 min for a triplicate determination.

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KW - persistent organic pollutants

KW - organochlorines

KW - total chlorine determination

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KW - Omega-3 supplements

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