Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time PCR

H. Hird, Georgina Louise Hold, J. Chisholm, P. Reece, V. J. Russell, J. Brown, R. Goodier, R. MacArthur

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A method is presented for the quantification of haddock in complex food matrices based on real-time PCR. The proportion of haddock muscle tissue to the numbers of a single copy gene has been shown to remain relatively constant, throughout the year and across a number of fishing grounds, using conventional muscle nitrogen content as a comparator. This indicates that quantification of fish, based upon nitrogen content or the numbers of a single copy gene, would return data with a similar degree of accuracy. Thus, a haddock-specific, real-time PCR primer and probe set was designed and optimised, and when used in conjunction with a haddock calibration curve was shown to be able to quantify model samples to within 7% of the true percentage. This is the first report of a real-time assay for the quantification of white fish, which would allow enforcement of the legislation in a complex food matrix containing haddock with other fish species.
Original languageEnglish
Pages (from-to)633-637
Number of pages5
JournalEuropean Food Research and Technology
Volume220
Issue number5-6
DOIs
Publication statusPublished - 2005

Fingerprint

Melanogrammus aeglefinus
haddock
Real-Time Polymerase Chain Reaction
Fishes
quantitative polymerase chain reaction
Nitrogen
food matrix
Food
Muscles
nitrogen content
Legislation
Calibration
Genes
fish
methodology
fishery resources
muscle tissues
laws and regulations
calibration
genes

Keywords

  • haddock
  • real-time polymerase chain reaction
  • quantification
  • nitrogen content
  • single copy gene
  • polymerase-chain-reaction
  • food-products
  • identification
  • fish

Cite this

Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time PCR. / Hird, H.; Hold, Georgina Louise; Chisholm, J.; Reece, P.; Russell, V. J.; Brown, J.; Goodier, R.; MacArthur, R.

In: European Food Research and Technology, Vol. 220, No. 5-6, 2005, p. 633-637.

Research output: Contribution to journalArticle

Hird, H. ; Hold, Georgina Louise ; Chisholm, J. ; Reece, P. ; Russell, V. J. ; Brown, J. ; Goodier, R. ; MacArthur, R. / Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time PCR. In: European Food Research and Technology. 2005 ; Vol. 220, No. 5-6. pp. 633-637.
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