Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time PCR

H. Hird, Georgina Louise Hold, J. Chisholm, P. Reece, V. J. Russell, J. Brown, R. Goodier, R. MacArthur

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38 Citations (Scopus)

Abstract

A method is presented for the quantification of haddock in complex food matrices based on real-time PCR. The proportion of haddock muscle tissue to the numbers of a single copy gene has been shown to remain relatively constant, throughout the year and across a number of fishing grounds, using conventional muscle nitrogen content as a comparator. This indicates that quantification of fish, based upon nitrogen content or the numbers of a single copy gene, would return data with a similar degree of accuracy. Thus, a haddock-specific, real-time PCR primer and probe set was designed and optimised, and when used in conjunction with a haddock calibration curve was shown to be able to quantify model samples to within 7% of the true percentage. This is the first report of a real-time assay for the quantification of white fish, which would allow enforcement of the legislation in a complex food matrix containing haddock with other fish species.
Original languageEnglish
Pages (from-to)633-637
Number of pages5
JournalEuropean Food Research and Technology
Volume220
Issue number5-6
DOIs
Publication statusPublished - 2005

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Keywords

  • haddock
  • real-time polymerase chain reaction
  • quantification
  • nitrogen content
  • single copy gene
  • polymerase-chain-reaction
  • food-products
  • identification
  • fish

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