Development of a monoclonal antibody-based enzyme-linked immunoabsorbent assay for the binding of gp130 to the IL-6/IL-6R complex and its competitive inhibition

Jürgen Scheller; Marina Kovaleva; Björn Rabe; Jutta Eichler; Karl-Josef Kallen; Stefan Rose-John; Marina Kovaleva

doi:10.1016/j.jim.2004.05.002

Development of a monoclonal antibody-based enzyme-linked immunoabsorbent assay for the binding of gp130 to the IL-6/IL-6R complex and its competitive inhibition

Jürgen Scheller, Marina Kovaleva, Björn Rabe, Jutta Eichler, Karl-Josef Kallen, Stefan Rose-John, Marina Kovaleva

Medical Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The proinflammatory cytokine IL-6 binds to the membrane bound or soluble IL-6 receptor (IL-6R) and activates an intracellular signaling cascade after complex formation with two gp130 molecules. These mediate general homeostasis and orchestrates the immune response during disease. Trans-signalling via the soluble IL-6R has importance for the development and maintenance of human diseases like Crohn's disease, peritonitis and rheumatoid arthritis. We have developed an enzyme-linked immunoabsorbent assay (ELISA) that detects the binding of gp130 to the IL-6/sIL-6R complex. Competitive binding of sgp130-Fc, viral IL-6, and the inhibitory drug Suramin to gp130 has been demonstrated. The assay can be used for high-throughput screening of peptide and chemical compound libraries for the identification of new agonists and antagonists of the binding between gp130 and IL-6/sIL-6R.

Original language	English
Pages (from-to)	93-100
Number of pages	8
Journal	Journal of Immunological Methods
Volume	291
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jim.2004.05.002
Publication status	Published - Aug 2004

Keywords

Antibodies, Monoclonal
Antigens, CD
Binding, Competitive
Cell Line
Cytokine Receptor gp130
Enzyme-Linked Immunosorbent Assay
Humans
Interleukin-6
Macromolecular Substances
Membrane Glycoproteins
Protein Binding
Receptors, Interleukin-6

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jim.2004.05.002

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@article{087132ac78f7408f81175390c819ad5f,

title = "Development of a monoclonal antibody-based enzyme-linked immunoabsorbent assay for the binding of gp130 to the IL-6/IL-6R complex and its competitive inhibition",

abstract = "The proinflammatory cytokine IL-6 binds to the membrane bound or soluble IL-6 receptor (IL-6R) and activates an intracellular signaling cascade after complex formation with two gp130 molecules. These mediate general homeostasis and orchestrates the immune response during disease. Trans-signalling via the soluble IL-6R has importance for the development and maintenance of human diseases like Crohn's disease, peritonitis and rheumatoid arthritis. We have developed an enzyme-linked immunoabsorbent assay (ELISA) that detects the binding of gp130 to the IL-6/sIL-6R complex. Competitive binding of sgp130-Fc, viral IL-6, and the inhibitory drug Suramin to gp130 has been demonstrated. The assay can be used for high-throughput screening of peptide and chemical compound libraries for the identification of new agonists and antagonists of the binding between gp130 and IL-6/sIL-6R.",

keywords = "Antibodies, Monoclonal, Antigens, CD, Binding, Competitive, Cell Line, Cytokine Receptor gp130, Enzyme-Linked Immunosorbent Assay, Humans, Interleukin-6, Macromolecular Substances, Membrane Glycoproteins, Protein Binding, Receptors, Interleukin-6",

author = "J{\"u}rgen Scheller and Marina Kovaleva and Bj{\"o}rn Rabe and Jutta Eichler and Karl-Josef Kallen and Stefan Rose-John and Marina Kovaleva",

year = "2004",

month = aug,

doi = "10.1016/j.jim.2004.05.002",

language = "English",

volume = "291",

pages = "93--100",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Development of a monoclonal antibody-based enzyme-linked immunoabsorbent assay for the binding of gp130 to the IL-6/IL-6R complex and its competitive inhibition

AU - Scheller, Jürgen

AU - Kovaleva, Marina

AU - Rabe, Björn

AU - Eichler, Jutta

AU - Kallen, Karl-Josef

AU - Rose-John, Stefan

AU - Kovaleva, Marina

PY - 2004/8

Y1 - 2004/8

N2 - The proinflammatory cytokine IL-6 binds to the membrane bound or soluble IL-6 receptor (IL-6R) and activates an intracellular signaling cascade after complex formation with two gp130 molecules. These mediate general homeostasis and orchestrates the immune response during disease. Trans-signalling via the soluble IL-6R has importance for the development and maintenance of human diseases like Crohn's disease, peritonitis and rheumatoid arthritis. We have developed an enzyme-linked immunoabsorbent assay (ELISA) that detects the binding of gp130 to the IL-6/sIL-6R complex. Competitive binding of sgp130-Fc, viral IL-6, and the inhibitory drug Suramin to gp130 has been demonstrated. The assay can be used for high-throughput screening of peptide and chemical compound libraries for the identification of new agonists and antagonists of the binding between gp130 and IL-6/sIL-6R.

AB - The proinflammatory cytokine IL-6 binds to the membrane bound or soluble IL-6 receptor (IL-6R) and activates an intracellular signaling cascade after complex formation with two gp130 molecules. These mediate general homeostasis and orchestrates the immune response during disease. Trans-signalling via the soluble IL-6R has importance for the development and maintenance of human diseases like Crohn's disease, peritonitis and rheumatoid arthritis. We have developed an enzyme-linked immunoabsorbent assay (ELISA) that detects the binding of gp130 to the IL-6/sIL-6R complex. Competitive binding of sgp130-Fc, viral IL-6, and the inhibitory drug Suramin to gp130 has been demonstrated. The assay can be used for high-throughput screening of peptide and chemical compound libraries for the identification of new agonists and antagonists of the binding between gp130 and IL-6/sIL-6R.

KW - Antibodies, Monoclonal

KW - Antigens, CD

KW - Binding, Competitive

KW - Cell Line

KW - Cytokine Receptor gp130

KW - Enzyme-Linked Immunosorbent Assay

KW - Humans

KW - Interleukin-6

KW - Macromolecular Substances

KW - Membrane Glycoproteins

KW - Protein Binding

KW - Receptors, Interleukin-6

U2 - 10.1016/j.jim.2004.05.002

DO - 10.1016/j.jim.2004.05.002

M3 - Article

C2 - 15345308

SN - 0022-1759

VL - 291

SP - 93

EP - 100

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

Development of a monoclonal antibody-based enzyme-linked immunoabsorbent assay for the binding of gp130 to the IL-6/IL-6R complex and its competitive inhibition

Abstract

Keywords

UN SDGs

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