Development of a novel, bioluminescence-based, fungal bioassay for toxicity testing

Hedda J. Weitz, Colin D. Campbell, Ken Killham

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Naturally bioluminescent fungi, Armillaria mellea and Mycena citricolor , were used to develop a novel, bioluminescence-based bioassay for toxicity testing. Bioassays were carried out to assess the toxicity of 3,5-dichlorophenol (3,5-DCP), pentachlorophenol (PCP), copper and zinc. The results suggested that 60 min was a suitable exposure time for the bioassay. Light reduction was observed in response to 3,5-DCP, PCP and Cu for both A. mellea and M. citricolor , but to Zn only for A. mellea . Armillaria mellea was significantly less sensitive to 3,5-DCP and PCP than M. citricolor. The EC50 values for A. mellea and M. citricolor were similar to EC50 values for 3,5-DCP, PCP and Cu (but not Zn) of bioluminescence-based bacterial biosensors. They were also similar to EC50 values for Cu and Zn of a bioluminescence-based yeast biosensor. The results highlighted the importance of using both prokaryotic and eukaryotic biosensors. The novel bioassay provides a rapid and sensitive method to assess bioavailability of pollutants as well as a method to determine their toxicity to filamentous fungi. It also expands the range of organisms that can be used for bioluminescence-based toxicity testing by complementing existing biosensors.

Original languageEnglish
Pages (from-to)422-429
Number of pages8
JournalEnvironmental Microbiology
Volume4
Issue number7
DOIs
Publication statusPublished - Jul 2002

Keywords

  • ectomycorrhizal fungi
  • biosensors
  • metals
  • pentachlorophenol

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