Abstract
We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryll-coenzyme A (CoA) CoA transferase genes. This pathway is present in numerically important groups of butyrate producers within the human colon, and thus this assay estimates the butyrate-producing ability of the microbiota.
Original language | English |
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Pages (from-to) | 2009-2012 |
Number of pages | 4 |
Journal | Applied and Environmental Microbiology |
Volume | 73 |
Issue number | 6 |
DOIs | |
Publication status | Published - Mar 2007 |
Keywords
- human colon
- human gut
- resistant starch
- phylogeny
- spp.