Development of a Semiquantitative Degenerate Real-Time PCR-Based Assay for Estimation of Numbers of Butyryl-Coenzyme A (CoA) CoA Transferase Genes in Complex Bacterial Samples

Petra Louis (Corresponding Author), Harry James Flint

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryll-coenzyme A (CoA) CoA transferase genes. This pathway is present in numerically important groups of butyrate producers within the human colon, and thus this assay estimates the butyrate-producing ability of the microbiota.

Original languageEnglish
Pages (from-to)2009-2012
Number of pages4
JournalApplied and Environmental Microbiology
Volume73
Issue number6
DOIs
Publication statusPublished - Mar 2007

Keywords

  • human colon
  • human gut
  • resistant starch
  • phylogeny
  • spp.

Cite this

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abstract = "We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryll-coenzyme A (CoA) CoA transferase genes. This pathway is present in numerically important groups of butyrate producers within the human colon, and thus this assay estimates the butyrate-producing ability of the microbiota.",
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AB - We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryll-coenzyme A (CoA) CoA transferase genes. This pathway is present in numerically important groups of butyrate producers within the human colon, and thus this assay estimates the butyrate-producing ability of the microbiota.

KW - human colon

KW - human gut

KW - resistant starch

KW - phylogeny

KW - spp.

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DO - 10.1128/AEM.02561-06

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