Development of an acute and chronic ecotoxicity assay using lux-marked Rhizobium legunninosarum biovar trifolii

G. I. Paton*, G. Palmer, M. Burton, E. A.S. Rattray, S. P. McGrath, L. A. Glover, K. Killham

*Corresponding author for this work

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A soil isolate of Rhizobium leguminosarum by trifolii was marked with a lux CDABE gene cassette to enable the expression of bioluminiscence. The suitability of the bacterium as a soil pollution biosensor was assessed using acute and chronic assays. Bacterial bioluminescence responded sensitively to the metals studied. The order of sensitivity was found to be Cd > Ni = Zn > Cu for the acute test and Cd > Ni = Zn = Cu for the chronic test. The sensitive response of the biosensor highlighted its potential for use as an indicator of soil pollution.

Original languageEnglish
Pages (from-to)296-300
Number of pages5
JournalLetters in Applied Microbiology
Volume24
Issue number4
DOIs
Publication statusPublished - 1 Jan 1997

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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