Development of microsatellite PCR typing methodology for the sea louse, Lepeophtheirus salmonis (Kroyer)

Damien Nolan, Samuel Allen Moore Martin, Y Kelly, K Glennon, R Palmer, T Smith, G P McCormack, R Powell

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In order to develop a microsatellite typing system for Lepeophtheirus salmonis (Kroyer), a DNA preparation method for individual sea lice suitable for analysis by polymerase chain reaction (PCR) was designed, and the DNA sequences of 50 L. salmonis microsatellite elements were determined. The microsatellites were composed of 60% perfect, 25% imperfect, and 15% compound repeats. Based on the flanking DNA sequences, four microsatellite-PCR assays were optimized and used in a pilot study to analyse L. salmonis samples collected in Ireland, Norway and Scotland. Two of the microsatellite-PCR assays targeted polymorphic loci amplifying seven and 10 alleles respectively. The results showed that microsatellite-PCR typing could detect genetic variation both within and between the L. salmonis groups, and also was capable of amplifying group-specific alleles.

Original languageEnglish
Pages (from-to)815-822
Number of pages8
JournalAquaculture Research
Volume31
Issue number11
DOIs
Publication statusPublished - Nov 2000

Keywords

  • Lepeophtheirus salmonis
  • sea louse
  • microsatellites
  • PCR
  • Eukaryotic genomes
  • human DNA
  • polymorphisms
  • libraries
  • sequences
  • mutation
  • markers
  • LOCI
  • wild

Cite this

Development of microsatellite PCR typing methodology for the sea louse, Lepeophtheirus salmonis (Kroyer). / Nolan, Damien; Martin, Samuel Allen Moore; Kelly, Y ; Glennon, K ; Palmer, R ; Smith, T ; McCormack, G P ; Powell, R .

In: Aquaculture Research, Vol. 31, No. 11, 11.2000, p. 815-822.

Research output: Contribution to journalArticle

Nolan, Damien ; Martin, Samuel Allen Moore ; Kelly, Y ; Glennon, K ; Palmer, R ; Smith, T ; McCormack, G P ; Powell, R . / Development of microsatellite PCR typing methodology for the sea louse, Lepeophtheirus salmonis (Kroyer). In: Aquaculture Research. 2000 ; Vol. 31, No. 11. pp. 815-822.
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