Abstract
The muscle larva of Trichinella spiralis is an intracellular parasite of mammalian skeletal muscle, encapsulating within a portion of the myofiber and resulting in muscle de-differentiation. Parasite-derived factors secreted or excreted by the muscle larva are thought to play a role in the formation of the host-parasite complex and in the induction of changes in the host cell. We screened a library enriched for T spiralis-specific cDNAs and identified a clone encoding a protein with similarity to a predicted secreted or extracellular Caenorhabditis elegans protein. The region of similarity included a conserved cysteine-glycine (CCG) domain, which we have identified as being nematode-specific. This domain is present in the predicted T spiralis protein, Ts-CCG-1, and in a second protein, Ts-CCG-2, which we identified from subsequent analysis. We showed that while the Ts-ccg-1 gene is constitutively expressed, Ts-ccg-2 gene expression is restricted to the muscle L1 larva. Both predicted proteins contain an N-terminal signal peptide and we subsequently confirmed by MALDI-TOF mass spectrometric analyses of excretory/secretory peptide spots excised from two-dimensional gels that Ts-CCG-2 is secreted. (C) 2004 Elsevier B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 257-266 |
Number of pages | 9 |
Journal | Molecular and Biochemical Parasitology |
Volume | 134 |
Issue number | 2 |
Early online date | 29 Jan 2004 |
DOIs | |
Publication status | Published - Apr 2004 |
Keywords
- Trichinella
- nematode
- muscle larva
- excretory/secretory
- conserved cysteine-glycine domain
- mass spectrometry
- infected muscle-cells
- parasitic nematode
- differential expression
- molecular-cloning
- in-vitro
- larvae
- pseudospiralis
- products
- host
- gene