Developmental regulation and secretion of nematode-specific cysteine-glycine domain proteins in Trichinella spiralis

Daniel Christopher Gare, J. Boyd, Bernadette Connolly

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


The muscle larva of Trichinella spiralis is an intracellular parasite of mammalian skeletal muscle, encapsulating within a portion of the myofiber and resulting in muscle de-differentiation. Parasite-derived factors secreted or excreted by the muscle larva are thought to play a role in the formation of the host-parasite complex and in the induction of changes in the host cell. We screened a library enriched for T spiralis-specific cDNAs and identified a clone encoding a protein with similarity to a predicted secreted or extracellular Caenorhabditis elegans protein. The region of similarity included a conserved cysteine-glycine (CCG) domain, which we have identified as being nematode-specific. This domain is present in the predicted T spiralis protein, Ts-CCG-1, and in a second protein, Ts-CCG-2, which we identified from subsequent analysis. We showed that while the Ts-ccg-1 gene is constitutively expressed, Ts-ccg-2 gene expression is restricted to the muscle L1 larva. Both predicted proteins contain an N-terminal signal peptide and we subsequently confirmed by MALDI-TOF mass spectrometric analyses of excretory/secretory peptide spots excised from two-dimensional gels that Ts-CCG-2 is secreted. (C) 2004 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)257-266
Number of pages9
JournalMolecular and Biochemical Parasitology
Issue number2
Early online date29 Jan 2004
Publication statusPublished - Apr 2004


  • Trichinella
  • nematode
  • muscle larva
  • excretory/secretory
  • conserved cysteine-glycine domain
  • mass spectrometry
  • infected muscle-cells
  • parasitic nematode
  • differential expression
  • molecular-cloning
  • in-vitro
  • larvae
  • pseudospiralis
  • products
  • host
  • gene


Dive into the research topics of 'Developmental regulation and secretion of nematode-specific cysteine-glycine domain proteins in Trichinella spiralis'. Together they form a unique fingerprint.

Cite this