Abstract
A method based on dialysis in the presence of histidine, and subsequent copper adsorption and preconcentration using Chelex-100 resin, has been developed for selective extraction of exchangeable copper from blood plasma. The method was verified by comparative analysis using two different instrumental techniques, ICP-MS and AAS. The results obtained were in excellent agreement. Accurate quantification of the exchangeable copper and the firmly bound copper was achieved. The method was further validated by analysis of a lyophilised human serum certified reference material.
| Original language | English |
|---|---|
| Pages (from-to) | 777-780 |
| Number of pages | 4 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 379 |
| Issue number | 5-6 |
| DOIs | |
| Publication status | Published - Jul 2004 |
| Event | 3rd International Conference on Instrumental Methods of Analysis - Thessaloniki, Greece Duration: 23 Sept 2003 → 27 Sept 2003 |
Bibliographical note
From the issue entitled "Instrumental Methods of Analysis"Keywords
- exchangeable copper
- dialysis
- Chelex-100
- ICP-MS
- atomic absorption spectrometry
- blood plasma
- serum
- zinc
- speciation
- binding