Dialysis-Chelex method for determination of exchangeable copper in human plasma

Tony I. Venelinov, I. M. Davies, John Hamilton Beattie

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    A method based on dialysis in the presence of histidine, and subsequent copper adsorption and preconcentration using Chelex-100 resin, has been developed for selective extraction of exchangeable copper from blood plasma. The method was verified by comparative analysis using two different instrumental techniques, ICP-MS and AAS. The results obtained were in excellent agreement. Accurate quantification of the exchangeable copper and the firmly bound copper was achieved. The method was further validated by analysis of a lyophilised human serum certified reference material.

    Original languageEnglish
    Pages (from-to)777-780
    Number of pages4
    JournalAnalytical and Bioanalytical Chemistry
    Volume379
    Issue number5-6
    DOIs
    Publication statusPublished - Jul 2004
    Event3rd International Conference on Instrumental Methods of Analysis - Thessaloniki, Greece
    Duration: 23 Sep 200327 Sep 2003

    Keywords

    • exchangeable copper
    • dialysis
    • Chelex-100
    • ICP-MS
    • atomic absorption spectrometry
    • blood plasma
    • serum
    • zinc
    • speciation
    • binding

    Cite this

    Dialysis-Chelex method for determination of exchangeable copper in human plasma. / Venelinov, Tony I.; Davies, I. M.; Beattie, John Hamilton.

    In: Analytical and Bioanalytical Chemistry, Vol. 379, No. 5-6, 07.2004, p. 777-780.

    Research output: Contribution to journalArticle

    Venelinov, Tony I. ; Davies, I. M. ; Beattie, John Hamilton. / Dialysis-Chelex method for determination of exchangeable copper in human plasma. In: Analytical and Bioanalytical Chemistry. 2004 ; Vol. 379, No. 5-6. pp. 777-780.
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    AU - Davies, I. M.

    AU - Beattie, John Hamilton

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    PY - 2004/7

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    N2 - A method based on dialysis in the presence of histidine, and subsequent copper adsorption and preconcentration using Chelex-100 resin, has been developed for selective extraction of exchangeable copper from blood plasma. The method was verified by comparative analysis using two different instrumental techniques, ICP-MS and AAS. The results obtained were in excellent agreement. Accurate quantification of the exchangeable copper and the firmly bound copper was achieved. The method was further validated by analysis of a lyophilised human serum certified reference material.

    AB - A method based on dialysis in the presence of histidine, and subsequent copper adsorption and preconcentration using Chelex-100 resin, has been developed for selective extraction of exchangeable copper from blood plasma. The method was verified by comparative analysis using two different instrumental techniques, ICP-MS and AAS. The results obtained were in excellent agreement. Accurate quantification of the exchangeable copper and the firmly bound copper was achieved. The method was further validated by analysis of a lyophilised human serum certified reference material.

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    KW - Chelex-100

    KW - ICP-MS

    KW - atomic absorption spectrometry

    KW - blood plasma

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    KW - zinc

    KW - speciation

    KW - binding

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