Dietary fibers inhibit obesity in mice, but host responses in the cecum and liver appear unrelated to fiber-specific changes in cecal bacterial taxonomic composition

Janice E Drew (Corresponding Author), Nicole Reichardt, Lynda M Williams, Claus-Dieter Mayer, Alan W. Walker, Andrew J Farquharson, Stavroula Kastora, Freda Farquharson, Graeme Milligan, Douglas J. Morrison, Tom Preston, Harry J. Flint, Petra Louis

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Abstract

Dietary fibers (DF) can prevent obesity in rodents fed a high-fat diet (HFD). Their mode of action is not fully elucidated, but the gut microbiota have been implicated. This study aimed to identify the effects of seven dietary fibers (barley beta-glucan, apple pectin, inulin, inulin acetate ester, inulin propionate ester, inulin butyrate ester or a combination of inulin propionate ester and inulin butyrate ester) effective in preventing diet-induced obesity and links to differences in cecal bacteria and host gene expression. Mice (n = 12) were fed either a low-fat diet (LFD), HFD or a HFD supplemented with the DFs, barley beta-glucan, apple pectin, inulin, inulin acetate ester, inulin propionate ester, inulin butyrate ester or a combination of inulin propionate ester and inulin butyrate ester for 8 weeks. Cecal bacteria were determined by Illumina MiSeq sequencing of 16S rRNA gene amplicons. Host responses, body composition, metabolic markers and gene transcription (cecum and liver) were assessed post intervention. HFD mice showed increased adiposity, while all of the DFs prevented weight gain. DF specific differences in cecal bacteria were observed. Results indicate that diverse DFs prevent weight gain on a HFD, despite giving rise to different cecal bacteria profiles. Conversely, common host responses to dietary fiber observed are predicted to be important in improving barrier function and genome stability in the gut, maintaining energy homeostasis and reducing HFD induced inflammatory responses in the liver.
Original languageEnglish
Article number15566
JournalScientific Reports
Volume8
DOIs
Publication statusPublished - 22 Oct 2018

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Inulin
Cecum
Dietary Fiber
Obesity
Esters
Liver
High Fat Diet
Butyrates
Propionates
Bacteria
beta-Glucans
Malus
Hordeum
Weight Gain
Acetates
Reducing Diet
Fat-Restricted Diet
Genomic Instability
Adiposity
Body Composition

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Dietary fibers inhibit obesity in mice, but host responses in the cecum and liver appear unrelated to fiber-specific changes in cecal bacterial taxonomic composition. / Drew, Janice E (Corresponding Author); Reichardt, Nicole; Williams, Lynda M; Mayer, Claus-Dieter; Walker, Alan W.; Farquharson, Andrew J; Kastora, Stavroula; Farquharson, Freda; Milligan, Graeme ; Morrison, Douglas J. ; Preston, Tom ; Flint, Harry J.; Louis, Petra.

In: Scientific Reports, Vol. 8, 15566, 22.10.2018.

Research output: Contribution to journalArticle

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