Dietary fish oil supplements modify ruminal biohydrogenation, alter the flow of fatty acids at the omasum, and induce changes in the ruminal Butyrivibrio population in lactating cows

Kevin J. Shingfield, Piia Kairenius, Anu Arola, Stefan Muetzel, Delphine Paillard, Seppo Ahvenjarvi, Aila Vanhatalo, Pekka Huhtanen, Vesa Toivonen, Mikko Griinari, R. John Wallace

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Abstract

Four lactating cows fitted with ruminal cannulae and fed a grass silage-based diet were used in a 4 x 4 Latin square with 28-d periods to investigate the effects of incremental dietary fish oil (FO) supplementation (0, 75, 150, or 300 g/d) on the flow of fatty acids at the omasum and populations of rumen bacteria capable of biohydrogenation. FO decreased silage intake and ruminal volatile fatty acid concentrations and promoted an increase in molar butyrate and propionate proportions at the expense of acetate. Extensive ruminal biohydrogenation of 20:5(n-3) and 22:6(n-3) resulted in corresponding increases in numerous 20- and 22-carbon unsaturated fatty acids at the omasum. Omasal flow of several 20-, 21-, and 22-carbon all-cis (n-3) PUFA exceeded the intake from FO. Supplements of FO also induced a dose-dependent decrease in 18:0 and increased trans 18:1 and trans 18:2 flow at the omasum. Trans-11 was the major 18:1 intermediate in digesta, while FO induced quadratic increases in trans-10 18:1 flow, reaching a maximum of 300 g/d. FO had no substantial influence on omasal flow of CLA. Results suggest that one or more fatty acids in FO inhibit the reduction of trans-18:1 and trans-18:2 intermediates by ruminal microorganisms. qPCR based on 16S rRNA genes in omasal digesta indicated that key Butyrivibrio spp. declined linearly in response to FO. Dose-dependent increases in ruminal outflow of biohydrogenation intermediates containing one or more trans double bonds in response to FO has major implications for host metabolism and the nutritional quality of ruminant foods
Original languageEnglish
Pages (from-to)1437-1448
Number of pages12
JournalThe Journal of Nutrition
Volume142
Issue number8
Early online date27 Jun 2012
DOIs
Publication statusPublished - 1 Aug 2012

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Shingfield, K. J., Kairenius, P., Arola, A., Muetzel, S., Paillard, D., Ahvenjarvi, S., Vanhatalo, A., Huhtanen, P., Toivonen, V., Griinari, M., & Wallace, R. J. (2012). Dietary fish oil supplements modify ruminal biohydrogenation, alter the flow of fatty acids at the omasum, and induce changes in the ruminal Butyrivibrio population in lactating cows. The Journal of Nutrition, 142(8), 1437-1448. https://doi.org/10.3945/jn.112.158576