Dietary selenium levels determine epidermal Langerhans cell numbers in mice

T S Rafferty, M Norval, A El-Ghorr, G J Beckett, John Arthur, Fergus Nicol, J,A,A Hunter, R.C McKenzie

Research output: Contribution to journalArticle

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Abstract

Selenium (Se) is a dietary trace element that is essential for effective immunity and protection from oxidative damage induced by ultraviolet radiation (UVR). Langerhans cells (LC) represent the major antigen-presenting cells resident in the epidermis; a proportion migrate from the skin to the draining lymph nodes in response to UVR. Because it is known that Se deficiency impairs immune function, we determined what effect this has on LC numbers. CH3/HeN mice were weaned at 3 wk and placed on diets containing <0.005 ppm of Se (Se deficient) or 0.1 ppm of Se (Se adequate, control mice). After 5 wk on the diet, the epidermal LC numbers in the Se-adequate group were 966 +/- 51 cells/mm(2) and LC counts in the epidermis of the Se-deficient mice were 49% lower (p<0.05). Glutathione peroxidase-I (GPx) activity was measured in the epidermis, lymph nodes, and liver. In the epidermis, the activity of GPx in the Se-deficient mice was only 39% (p<0.01) of that seen in epidermis from Se-adequate mice (1.732 U/mg protein). The mice were then irradiated with one dose of 1440 J/m(2) Of broadband UVB or mock irradiated. After 24 h, the decrease in LC number after UVB was greater in the Se-adequate mice, (40% decrease) compared to the Se-deficient group (10%). Thus, Se deficiency reduces epidermal LC numbers, an effect that might compromise cutaneous immunity.

Original languageEnglish
Pages (from-to)161-171
Number of pages11
JournalBiological Trace Element Research
Volume92
Issue number2
DOIs
Publication statusPublished - May 2003

Keywords

  • Langerhans cells
  • selenium
  • antigen-presenting cells
  • antioxidants
  • tumor necrosis factor-alpha
  • glutathione peroxidase
  • ultraviolet radiation
  • NECROSIS-FACTOR-ALPHA
  • INDUCED SKIN CARCINOGENESIS
  • DRAINING LYMPH-NODES
  • ULTRAVIOLET-RADIATION
  • CONTACT HYPERSENSITIVITY
  • GLUTATHIONE-PEROXIDASE
  • NATURAL-KILLER
  • IMMUNE-SYSTEM
  • MIGRATION
  • KERATINOCYTES
  • Langerhans cells
  • selenium
  • antigen-presenting cells
  • antioxidants
  • tumor necrosis factor
  • glutathione peroxidase
  • ultraviolet radiation

Cite this

Rafferty, T. S., Norval, M., El-Ghorr, A., Beckett, G. J., Arthur, J., Nicol, F., ... McKenzie, R. C. (2003). Dietary selenium levels determine epidermal Langerhans cell numbers in mice. Biological Trace Element Research, 92(2), 161-171. https://doi.org/10.1385/BTER:92:2:161

Dietary selenium levels determine epidermal Langerhans cell numbers in mice. / Rafferty, T S ; Norval, M ; El-Ghorr, A ; Beckett, G J ; Arthur, John; Nicol, Fergus; Hunter, J,A,A; McKenzie, R.C.

In: Biological Trace Element Research, Vol. 92, No. 2, 05.2003, p. 161-171.

Research output: Contribution to journalArticle

Rafferty, TS, Norval, M, El-Ghorr, A, Beckett, GJ, Arthur, J, Nicol, F, Hunter, JAA & McKenzie, RC 2003, 'Dietary selenium levels determine epidermal Langerhans cell numbers in mice', Biological Trace Element Research, vol. 92, no. 2, pp. 161-171. https://doi.org/10.1385/BTER:92:2:161
Rafferty TS, Norval M, El-Ghorr A, Beckett GJ, Arthur J, Nicol F et al. Dietary selenium levels determine epidermal Langerhans cell numbers in mice. Biological Trace Element Research. 2003 May;92(2):161-171. https://doi.org/10.1385/BTER:92:2:161
Rafferty, T S ; Norval, M ; El-Ghorr, A ; Beckett, G J ; Arthur, John ; Nicol, Fergus ; Hunter, J,A,A ; McKenzie, R.C. / Dietary selenium levels determine epidermal Langerhans cell numbers in mice. In: Biological Trace Element Research. 2003 ; Vol. 92, No. 2. pp. 161-171.
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abstract = "Selenium (Se) is a dietary trace element that is essential for effective immunity and protection from oxidative damage induced by ultraviolet radiation (UVR). Langerhans cells (LC) represent the major antigen-presenting cells resident in the epidermis; a proportion migrate from the skin to the draining lymph nodes in response to UVR. Because it is known that Se deficiency impairs immune function, we determined what effect this has on LC numbers. CH3/HeN mice were weaned at 3 wk and placed on diets containing <0.005 ppm of Se (Se deficient) or 0.1 ppm of Se (Se adequate, control mice). After 5 wk on the diet, the epidermal LC numbers in the Se-adequate group were 966 +/- 51 cells/mm(2) and LC counts in the epidermis of the Se-deficient mice were 49{\%} lower (p<0.05). Glutathione peroxidase-I (GPx) activity was measured in the epidermis, lymph nodes, and liver. In the epidermis, the activity of GPx in the Se-deficient mice was only 39{\%} (p<0.01) of that seen in epidermis from Se-adequate mice (1.732 U/mg protein). The mice were then irradiated with one dose of 1440 J/m(2) Of broadband UVB or mock irradiated. After 24 h, the decrease in LC number after UVB was greater in the Se-adequate mice, (40{\%} decrease) compared to the Se-deficient group (10{\%}). Thus, Se deficiency reduces epidermal LC numbers, an effect that might compromise cutaneous immunity.",
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AU - Rafferty, T S

AU - Norval, M

AU - El-Ghorr, A

AU - Beckett, G J

AU - Arthur, John

AU - Nicol, Fergus

AU - Hunter, J,A,A

AU - McKenzie, R.C

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N2 - Selenium (Se) is a dietary trace element that is essential for effective immunity and protection from oxidative damage induced by ultraviolet radiation (UVR). Langerhans cells (LC) represent the major antigen-presenting cells resident in the epidermis; a proportion migrate from the skin to the draining lymph nodes in response to UVR. Because it is known that Se deficiency impairs immune function, we determined what effect this has on LC numbers. CH3/HeN mice were weaned at 3 wk and placed on diets containing <0.005 ppm of Se (Se deficient) or 0.1 ppm of Se (Se adequate, control mice). After 5 wk on the diet, the epidermal LC numbers in the Se-adequate group were 966 +/- 51 cells/mm(2) and LC counts in the epidermis of the Se-deficient mice were 49% lower (p<0.05). Glutathione peroxidase-I (GPx) activity was measured in the epidermis, lymph nodes, and liver. In the epidermis, the activity of GPx in the Se-deficient mice was only 39% (p<0.01) of that seen in epidermis from Se-adequate mice (1.732 U/mg protein). The mice were then irradiated with one dose of 1440 J/m(2) Of broadband UVB or mock irradiated. After 24 h, the decrease in LC number after UVB was greater in the Se-adequate mice, (40% decrease) compared to the Se-deficient group (10%). Thus, Se deficiency reduces epidermal LC numbers, an effect that might compromise cutaneous immunity.

AB - Selenium (Se) is a dietary trace element that is essential for effective immunity and protection from oxidative damage induced by ultraviolet radiation (UVR). Langerhans cells (LC) represent the major antigen-presenting cells resident in the epidermis; a proportion migrate from the skin to the draining lymph nodes in response to UVR. Because it is known that Se deficiency impairs immune function, we determined what effect this has on LC numbers. CH3/HeN mice were weaned at 3 wk and placed on diets containing <0.005 ppm of Se (Se deficient) or 0.1 ppm of Se (Se adequate, control mice). After 5 wk on the diet, the epidermal LC numbers in the Se-adequate group were 966 +/- 51 cells/mm(2) and LC counts in the epidermis of the Se-deficient mice were 49% lower (p<0.05). Glutathione peroxidase-I (GPx) activity was measured in the epidermis, lymph nodes, and liver. In the epidermis, the activity of GPx in the Se-deficient mice was only 39% (p<0.01) of that seen in epidermis from Se-adequate mice (1.732 U/mg protein). The mice were then irradiated with one dose of 1440 J/m(2) Of broadband UVB or mock irradiated. After 24 h, the decrease in LC number after UVB was greater in the Se-adequate mice, (40% decrease) compared to the Se-deficient group (10%). Thus, Se deficiency reduces epidermal LC numbers, an effect that might compromise cutaneous immunity.

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KW - KERATINOCYTES

KW - Langerhans cells

KW - selenium

KW - antigen-presenting cells

KW - antioxidants

KW - tumor necrosis factor

KW - glutathione peroxidase

KW - ultraviolet radiation

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DO - 10.1385/BTER:92:2:161

M3 - Article

VL - 92

SP - 161

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JO - Biological Trace Element Research

JF - Biological Trace Element Research

SN - 0163-4984

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