Dietary supplementation of Chlorella vulgaris ameliorates chronic sodium arsenite toxicity in Nile tilapia Oreochromis niloticus as revealed by histopathological, biochemical and immune gene expression analysis

Eman Zahran (Corresponding Author), Walaa Awadin, Engy Risha, Asmaa A. Khaled, Tiehui Wang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Arsenic toxicity in an aquatic environment is a major concern, and its elimination has become a global challenge. In the current study, histopathology, serum biomarkers and cytokine gene expression were comparatively examined in fish fed with a control diet or diets containing Chlorella vulgaris (Ch) after exposure to sodium arsenite (NaAsO2) in Nile tilapia (Oreochromis niloticus) with the aim of evaluating the protective role of Ch against arsenite-induced toxicity. Severe histopathological alterations were evident in fish exposed to 7 ppm (parts per million) arsenite for 21 days, compared to unexposed fish. Levels of serum biomarkers ALT, AST, ALP, urea and creatinine were increased, but the levels of Na+, total proteins, albumins and globulins were decreased. Moreover, the expression of all the cytokine genes examined, including IL-1β (7-fold), TNF-α (14-fold) and TGF-β1 (13-fold), were significantly upregulated after arsenite exposure. However, in fish fed with diets containing 5% or 10% Ch, the histopathological alterations in the gills, liver and head kidney were reduced, the biomarkers were stabilized, and the upregulation of cytokine gene expression was lowered, with the high Ch diet (10%) showing more prominent effects. These results suggest the protective and therapeutic roles of Ch as a feed supplement in Nile tilapia against arsenic induced toxicity.
Original languageEnglish
Pages (from-to)199-215
Number of pages17
JournalFisheries Science
Volume85
Issue number1
Early online date13 Nov 2018
DOIs
Publication statusPublished - 31 Jan 2019

Fingerprint

sodium arsenite
Chlorella vulgaris
Oreochromis niloticus
arsenites
dietary supplements
toxicity
biomarkers
gene expression
cytokines
arsenic
diet
fish feeds
feed supplements
interleukin-1
aquatic environment
fish
globulins
histopathology
creatinine
albumins

Keywords

  • fish
  • heavy metals
  • algae
  • immunity
  • enzymatic activity
  • tissue alterations
  • Enzymatic activity
  • Algae
  • Fish
  • Tissue alterations
  • Immunity
  • Heavy metals
  • LIVER-TISSUE
  • OXIDATIVE STRESS
  • EMBRYONIC EXPOSURE
  • HEAVY-METALS
  • COPPER EXPOSURE
  • CYTOKINE EXPRESSION
  • FRESH-WATER FISH
  • SUBLETHAL CONCENTRATION
  • SPARUS-AURATA
  • ODONTESTHES-BONARIENSIS

Cite this

Dietary supplementation of Chlorella vulgaris ameliorates chronic sodium arsenite toxicity in Nile tilapia Oreochromis niloticus as revealed by histopathological, biochemical and immune gene expression analysis. / Zahran, Eman (Corresponding Author); Awadin, Walaa; Risha, Engy; Khaled, Asmaa A.; Wang, Tiehui.

In: Fisheries Science, Vol. 85, No. 1, 31.01.2019, p. 199-215.

Research output: Contribution to journalArticle

@article{51fd7c9ec4144824bbf03cf102f087bb,
title = "Dietary supplementation of Chlorella vulgaris ameliorates chronic sodium arsenite toxicity in Nile tilapia Oreochromis niloticus as revealed by histopathological, biochemical and immune gene expression analysis",
abstract = "Arsenic toxicity in an aquatic environment is a major concern, and its elimination has become a global challenge. In the current study, histopathology, serum biomarkers and cytokine gene expression were comparatively examined in fish fed with a control diet or diets containing Chlorella vulgaris (Ch) after exposure to sodium arsenite (NaAsO2) in Nile tilapia (Oreochromis niloticus) with the aim of evaluating the protective role of Ch against arsenite-induced toxicity. Severe histopathological alterations were evident in fish exposed to 7 ppm (parts per million) arsenite for 21 days, compared to unexposed fish. Levels of serum biomarkers ALT, AST, ALP, urea and creatinine were increased, but the levels of Na+, total proteins, albumins and globulins were decreased. Moreover, the expression of all the cytokine genes examined, including IL-1β (7-fold), TNF-α (14-fold) and TGF-β1 (13-fold), were significantly upregulated after arsenite exposure. However, in fish fed with diets containing 5{\%} or 10{\%} Ch, the histopathological alterations in the gills, liver and head kidney were reduced, the biomarkers were stabilized, and the upregulation of cytokine gene expression was lowered, with the high Ch diet (10{\%}) showing more prominent effects. These results suggest the protective and therapeutic roles of Ch as a feed supplement in Nile tilapia against arsenic induced toxicity.",
keywords = "fish, heavy metals, algae, immunity, enzymatic activity, tissue alterations, Enzymatic activity, Algae, Fish, Tissue alterations, Immunity, Heavy metals, LIVER-TISSUE, OXIDATIVE STRESS, EMBRYONIC EXPOSURE, HEAVY-METALS, COPPER EXPOSURE, CYTOKINE EXPRESSION, FRESH-WATER FISH, SUBLETHAL CONCENTRATION, SPARUS-AURATA, ODONTESTHES-BONARIENSIS",
author = "Eman Zahran and Walaa Awadin and Engy Risha and Khaled, {Asmaa A.} and Tiehui Wang",
year = "2019",
month = "1",
day = "31",
doi = "10.1007/s12562-018-1274-6",
language = "English",
volume = "85",
pages = "199--215",
journal = "Fisheries Science",
issn = "1444-2906",
publisher = "Springer",
number = "1",

}

TY - JOUR

T1 - Dietary supplementation of Chlorella vulgaris ameliorates chronic sodium arsenite toxicity in Nile tilapia Oreochromis niloticus as revealed by histopathological, biochemical and immune gene expression analysis

AU - Zahran, Eman

AU - Awadin, Walaa

AU - Risha, Engy

AU - Khaled, Asmaa A.

AU - Wang, Tiehui

PY - 2019/1/31

Y1 - 2019/1/31

N2 - Arsenic toxicity in an aquatic environment is a major concern, and its elimination has become a global challenge. In the current study, histopathology, serum biomarkers and cytokine gene expression were comparatively examined in fish fed with a control diet or diets containing Chlorella vulgaris (Ch) after exposure to sodium arsenite (NaAsO2) in Nile tilapia (Oreochromis niloticus) with the aim of evaluating the protective role of Ch against arsenite-induced toxicity. Severe histopathological alterations were evident in fish exposed to 7 ppm (parts per million) arsenite for 21 days, compared to unexposed fish. Levels of serum biomarkers ALT, AST, ALP, urea and creatinine were increased, but the levels of Na+, total proteins, albumins and globulins were decreased. Moreover, the expression of all the cytokine genes examined, including IL-1β (7-fold), TNF-α (14-fold) and TGF-β1 (13-fold), were significantly upregulated after arsenite exposure. However, in fish fed with diets containing 5% or 10% Ch, the histopathological alterations in the gills, liver and head kidney were reduced, the biomarkers were stabilized, and the upregulation of cytokine gene expression was lowered, with the high Ch diet (10%) showing more prominent effects. These results suggest the protective and therapeutic roles of Ch as a feed supplement in Nile tilapia against arsenic induced toxicity.

AB - Arsenic toxicity in an aquatic environment is a major concern, and its elimination has become a global challenge. In the current study, histopathology, serum biomarkers and cytokine gene expression were comparatively examined in fish fed with a control diet or diets containing Chlorella vulgaris (Ch) after exposure to sodium arsenite (NaAsO2) in Nile tilapia (Oreochromis niloticus) with the aim of evaluating the protective role of Ch against arsenite-induced toxicity. Severe histopathological alterations were evident in fish exposed to 7 ppm (parts per million) arsenite for 21 days, compared to unexposed fish. Levels of serum biomarkers ALT, AST, ALP, urea and creatinine were increased, but the levels of Na+, total proteins, albumins and globulins were decreased. Moreover, the expression of all the cytokine genes examined, including IL-1β (7-fold), TNF-α (14-fold) and TGF-β1 (13-fold), were significantly upregulated after arsenite exposure. However, in fish fed with diets containing 5% or 10% Ch, the histopathological alterations in the gills, liver and head kidney were reduced, the biomarkers were stabilized, and the upregulation of cytokine gene expression was lowered, with the high Ch diet (10%) showing more prominent effects. These results suggest the protective and therapeutic roles of Ch as a feed supplement in Nile tilapia against arsenic induced toxicity.

KW - fish

KW - heavy metals

KW - algae

KW - immunity

KW - enzymatic activity

KW - tissue alterations

KW - Enzymatic activity

KW - Algae

KW - Fish

KW - Tissue alterations

KW - Immunity

KW - Heavy metals

KW - LIVER-TISSUE

KW - OXIDATIVE STRESS

KW - EMBRYONIC EXPOSURE

KW - HEAVY-METALS

KW - COPPER EXPOSURE

KW - CYTOKINE EXPRESSION

KW - FRESH-WATER FISH

KW - SUBLETHAL CONCENTRATION

KW - SPARUS-AURATA

KW - ODONTESTHES-BONARIENSIS

UR - http://www.mendeley.com/research/dietary-supplementation-chlorella-vulgaris-ameliorates-chronic-sodium-arsenite-toxicity-nile-tilapia

U2 - 10.1007/s12562-018-1274-6

DO - 10.1007/s12562-018-1274-6

M3 - Article

VL - 85

SP - 199

EP - 215

JO - Fisheries Science

JF - Fisheries Science

SN - 1444-2906

IS - 1

ER -