Differences between betaproteobacterial ammonia-oxidizing communities in marine sediments and those in overlying water

Thomas Erich Freitag, James Ivor Prosser

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

To assess links between betaproteobacterial ammonia-oxidizing bacteria (AOB) in marine sediment and in overlying water, communities in Loch Duich, Scotland, were characterized by analysis of clone libraries and denaturant gradient gel electrophoresis of 16S rRNA gene fragments. Nitrosospira cluster I-like sequences were isolated from both environments, but different sequence types dominated water and sediment samples. Detailed phylogenetic analysis of marine Nitrosospira cluster 1-like sequences in Loch Duich and surrounding regions suggests the existence of at least two different phylogenetic subgroups, potentially indicative of new lineages within the betaproteobacterial AOB, representing different marine ecotypes.

Original languageEnglish
Pages (from-to)3789-3793
Number of pages4
JournalApplied and Environmental Microbiology
Volume70
Issue number6
DOIs
Publication statusPublished - 2004

Keywords

  • 16S RIBOSOMAL-RNA
  • GRADIENT GEL-ELECTROPHORESIS
  • POLYMERASE CHAIN-REACTION
  • BETA-SUBDIVISION
  • CLASS PROTEOBACTERIA
  • MOLECULAR DIVERSITY
  • BACTERIA
  • DNA
  • SEQUENCES
  • PHYLOGENY

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