Differences between human subjects in the composition of the faecal bacterial community and faecal metabolism of linoleic acid

Estelle Devillard, Freda M. McIntosh, Delphine Paillard, Nadine A. Thomas, Kevin J. Shingfield, R. John Wallace

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Conjugated linoleic acid (CLA) is formed from linoleic acid (LA; cis-9,cis-12-18: 2) by intestinal bacteria. Different CLA isomers have different implications for human health. The aim of this study was to investigate LA metabolism and the CLA isomers formed in two individuals (V1 and V2) with different faecal metabolic characteristics, and to compare fatty acid metabolism with the microbial community composition. LA incubated with faecal samples was metabolized at similar rates with both subjects, but the products were different. LA was metabolized extensively to stearic acid (SA; 18: 0) in V1, with minor accumulation of CLA and more rapid accumulation of vaccenic acid (VA; trans-11-18: 1). CLA accumulation at 4 h was almost tenfold higher with V2, and little SA was formed. At least 12 different isomers of CLA were produced from LA by the colonic bacteria from the two individuals. The predominant (>75%) CLA isomer in V1 was rumenic acid (RA; cis-9,trans-11-18 : 2), whereas the concentrations of RA and trans-10,cis-12-18: 2 were similar with V2. Propionate and butyrate proportions in short-chain fatty acids were higher in V1. A 16S rRNA clone library from V1 contained mainly Bacteroidetes (54% of clones), whereas Firmicutes (66% of clones) predominated in V2. Both samples were devoid of bacteria related to Clostridium proteoclasticum, the only gut bacterium known to metabolize VA to SA. Thus, the CLA formed in the intestine of different individuals may differ according to their resident microbiota, with possibly important implications with respect to gut health.

Original languageEnglish
Pages (from-to)513-520
Number of pages8
JournalMicrobiology
Volume155
Issue number2
DOIs
Publication statusPublished - Feb 2009

Keywords

  • 16s ribosomal-RNA
  • polyunsaturated fatty-acids
  • human colon
  • human gut
  • fish-oil
  • intestinal microorganisms
  • gas-chromatography
  • gnotobiotic-rats
  • gene-expression
  • vaccenic acid

Cite this

Differences between human subjects in the composition of the faecal bacterial community and faecal metabolism of linoleic acid. / Devillard, Estelle; McIntosh, Freda M.; Paillard, Delphine; Thomas, Nadine A.; Shingfield, Kevin J.; Wallace, R. John.

In: Microbiology , Vol. 155, No. 2, 02.2009, p. 513-520.

Research output: Contribution to journalArticle

Devillard, Estelle ; McIntosh, Freda M. ; Paillard, Delphine ; Thomas, Nadine A. ; Shingfield, Kevin J. ; Wallace, R. John. / Differences between human subjects in the composition of the faecal bacterial community and faecal metabolism of linoleic acid. In: Microbiology . 2009 ; Vol. 155, No. 2. pp. 513-520.
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abstract = "Conjugated linoleic acid (CLA) is formed from linoleic acid (LA; cis-9,cis-12-18: 2) by intestinal bacteria. Different CLA isomers have different implications for human health. The aim of this study was to investigate LA metabolism and the CLA isomers formed in two individuals (V1 and V2) with different faecal metabolic characteristics, and to compare fatty acid metabolism with the microbial community composition. LA incubated with faecal samples was metabolized at similar rates with both subjects, but the products were different. LA was metabolized extensively to stearic acid (SA; 18: 0) in V1, with minor accumulation of CLA and more rapid accumulation of vaccenic acid (VA; trans-11-18: 1). CLA accumulation at 4 h was almost tenfold higher with V2, and little SA was formed. At least 12 different isomers of CLA were produced from LA by the colonic bacteria from the two individuals. The predominant (>75{\%}) CLA isomer in V1 was rumenic acid (RA; cis-9,trans-11-18 : 2), whereas the concentrations of RA and trans-10,cis-12-18: 2 were similar with V2. Propionate and butyrate proportions in short-chain fatty acids were higher in V1. A 16S rRNA clone library from V1 contained mainly Bacteroidetes (54{\%} of clones), whereas Firmicutes (66{\%} of clones) predominated in V2. Both samples were devoid of bacteria related to Clostridium proteoclasticum, the only gut bacterium known to metabolize VA to SA. Thus, the CLA formed in the intestine of different individuals may differ according to their resident microbiota, with possibly important implications with respect to gut health.",
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AU - Devillard, Estelle

AU - McIntosh, Freda M.

AU - Paillard, Delphine

AU - Thomas, Nadine A.

AU - Shingfield, Kevin J.

AU - Wallace, R. John

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N2 - Conjugated linoleic acid (CLA) is formed from linoleic acid (LA; cis-9,cis-12-18: 2) by intestinal bacteria. Different CLA isomers have different implications for human health. The aim of this study was to investigate LA metabolism and the CLA isomers formed in two individuals (V1 and V2) with different faecal metabolic characteristics, and to compare fatty acid metabolism with the microbial community composition. LA incubated with faecal samples was metabolized at similar rates with both subjects, but the products were different. LA was metabolized extensively to stearic acid (SA; 18: 0) in V1, with minor accumulation of CLA and more rapid accumulation of vaccenic acid (VA; trans-11-18: 1). CLA accumulation at 4 h was almost tenfold higher with V2, and little SA was formed. At least 12 different isomers of CLA were produced from LA by the colonic bacteria from the two individuals. The predominant (>75%) CLA isomer in V1 was rumenic acid (RA; cis-9,trans-11-18 : 2), whereas the concentrations of RA and trans-10,cis-12-18: 2 were similar with V2. Propionate and butyrate proportions in short-chain fatty acids were higher in V1. A 16S rRNA clone library from V1 contained mainly Bacteroidetes (54% of clones), whereas Firmicutes (66% of clones) predominated in V2. Both samples were devoid of bacteria related to Clostridium proteoclasticum, the only gut bacterium known to metabolize VA to SA. Thus, the CLA formed in the intestine of different individuals may differ according to their resident microbiota, with possibly important implications with respect to gut health.

AB - Conjugated linoleic acid (CLA) is formed from linoleic acid (LA; cis-9,cis-12-18: 2) by intestinal bacteria. Different CLA isomers have different implications for human health. The aim of this study was to investigate LA metabolism and the CLA isomers formed in two individuals (V1 and V2) with different faecal metabolic characteristics, and to compare fatty acid metabolism with the microbial community composition. LA incubated with faecal samples was metabolized at similar rates with both subjects, but the products were different. LA was metabolized extensively to stearic acid (SA; 18: 0) in V1, with minor accumulation of CLA and more rapid accumulation of vaccenic acid (VA; trans-11-18: 1). CLA accumulation at 4 h was almost tenfold higher with V2, and little SA was formed. At least 12 different isomers of CLA were produced from LA by the colonic bacteria from the two individuals. The predominant (>75%) CLA isomer in V1 was rumenic acid (RA; cis-9,trans-11-18 : 2), whereas the concentrations of RA and trans-10,cis-12-18: 2 were similar with V2. Propionate and butyrate proportions in short-chain fatty acids were higher in V1. A 16S rRNA clone library from V1 contained mainly Bacteroidetes (54% of clones), whereas Firmicutes (66% of clones) predominated in V2. Both samples were devoid of bacteria related to Clostridium proteoclasticum, the only gut bacterium known to metabolize VA to SA. Thus, the CLA formed in the intestine of different individuals may differ according to their resident microbiota, with possibly important implications with respect to gut health.

KW - 16s ribosomal-RNA

KW - polyunsaturated fatty-acids

KW - human colon

KW - human gut

KW - fish-oil

KW - intestinal microorganisms

KW - gas-chromatography

KW - gnotobiotic-rats

KW - gene-expression

KW - vaccenic acid

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DO - 10.1099/mic.0.023416-0

M3 - Article

VL - 155

SP - 513

EP - 520

JO - Microbiology

JF - Microbiology

SN - 1350-0872

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ER -