Differences in pH optima and calcium requirements for maturation of the prohormone convertases PC2 and PC3 indicates different intracellular locations for these events

K I Shennan, N A Taylor, J L Jermany, G Matthews, K Docherty

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

PC2 and PC3, which is also known as PC1, are subtilisin-like proteases that are involved in the intracellular processing of prohormones and proneuropeptides. Both enzymes are synthesized as propolypeptides that undergo proteolytic maturation within the secretory pathway. An in vitro translation/translocation system from Xenopus egg extracts was used to investigate mechanisms in the maturation of pro-PC3 and pro-PC2. Pro-PC3 underwent rapid (t1/2
Original languageEnglish
Pages (from-to)1402-7
Number of pages6
JournalThe Journal of Biological Chemistry
Volume270
Issue number3
Publication statusPublished - 20 Jan 1995

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Keywords

  • Amino Acid Sequence
  • Animals
  • Aspartic Acid Endopeptidases
  • Base Sequence
  • Biological Transport
  • Calcium
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Proprotein Convertase 2
  • Proprotein Convertases
  • Protein Processing, Post-Translational
  • RNA, Messenger
  • Subtilisins
  • Xenopus

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