Differences in structural and pain phenotypes in the sodium monoiodoacetate and meniscal transection models of osteoarthritis

P. I. Mapp (Corresponding Author), D. R. Sagar, S. Ashraf, J. J. Burston, S. Suri, V. Chapman, D. A. Walsh

Research output: Contribution to journalArticle

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Abstract

Objectives
To characterize differences in joint pathology and pain behavior between two rat models of osteoarthritis (OA) in order to inform selection of animal models for interventional studies.

Method
Knee OA was induced in Sprague Dawley rats by either meniscal transection (MNX) or intra-articular injection of monosodium iodoacetate (MIA). Controls were subjected to sham surgery or saline-injection. In a separate experiment, a single intra-articular injection of triamcinolone acetonide was administered 14 days after MNX or MIA arthritis induction. Pain behavior and joint pathology were quantified.

Results
Both models displayed synovial inflammation, chondropathy and osteophytosis. Chondropathy scores increased with time similarly in the two models. Inflammation and osteophyte scores were greater in MNX model compared to the MIA model. At day 49, the MNX model exhibited a greater number of channels crossing the osteochondral junction compared to all other groups. The MNX model exhibited greater weight bearing asymmetry compared to the MIA model, whereas the MIA model displayed more consistent hindpaw allodynia. Triamcinolone attenuated weight bearing asymmetry and distal allodynia to control levels in the MNX model, but distal allodynia was unaltered in the MIA model.

Conclusions
The comparison of the two models of OA in rats, using identical assessment tools has demonstrated that although both models display features of OA, there are differences between the models which may represent different aspects of human OA. Thus, model selection should be based on the pathological aspects of OA under investigation.
Original languageEnglish
Pages (from-to)1336-1345
Number of pages10
JournalOsteoarthritis and Cartilage
Volume21
Issue number9
Early online date22 Aug 2013
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Iodoacetates
Osteoarthritis
Sodium
Phenotype
Pain
Hyperalgesia
Intra-Articular Injections
Weight-Bearing
Arthralgia
Pathology
Inflammation
Osteophyte
Triamcinolone
Triamcinolone Acetonide
Bearings (structural)
Rats
Arthritis
Sprague Dawley Rats
Animal Models
Injections

Keywords

  • Osteoarthritis
  • Animal models
  • Pain
  • Inflammation
  • Glucocorticosteroids

Cite this

Differences in structural and pain phenotypes in the sodium monoiodoacetate and meniscal transection models of osteoarthritis. / Mapp, P. I. (Corresponding Author); Sagar, D. R.; Ashraf, S.; Burston, J. J.; Suri, S.; Chapman, V.; Walsh, D. A.

In: Osteoarthritis and Cartilage, Vol. 21, No. 9, 09.2013, p. 1336-1345.

Research output: Contribution to journalArticle

Mapp, P. I. ; Sagar, D. R. ; Ashraf, S. ; Burston, J. J. ; Suri, S. ; Chapman, V. ; Walsh, D. A. / Differences in structural and pain phenotypes in the sodium monoiodoacetate and meniscal transection models of osteoarthritis. In: Osteoarthritis and Cartilage. 2013 ; Vol. 21, No. 9. pp. 1336-1345.
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abstract = "ObjectivesTo characterize differences in joint pathology and pain behavior between two rat models of osteoarthritis (OA) in order to inform selection of animal models for interventional studies.MethodKnee OA was induced in Sprague Dawley rats by either meniscal transection (MNX) or intra-articular injection of monosodium iodoacetate (MIA). Controls were subjected to sham surgery or saline-injection. In a separate experiment, a single intra-articular injection of triamcinolone acetonide was administered 14 days after MNX or MIA arthritis induction. Pain behavior and joint pathology were quantified.ResultsBoth models displayed synovial inflammation, chondropathy and osteophytosis. Chondropathy scores increased with time similarly in the two models. Inflammation and osteophyte scores were greater in MNX model compared to the MIA model. At day 49, the MNX model exhibited a greater number of channels crossing the osteochondral junction compared to all other groups. The MNX model exhibited greater weight bearing asymmetry compared to the MIA model, whereas the MIA model displayed more consistent hindpaw allodynia. Triamcinolone attenuated weight bearing asymmetry and distal allodynia to control levels in the MNX model, but distal allodynia was unaltered in the MIA model.ConclusionsThe comparison of the two models of OA in rats, using identical assessment tools has demonstrated that although both models display features of OA, there are differences between the models which may represent different aspects of human OA. Thus, model selection should be based on the pathological aspects of OA under investigation.",
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AU - Mapp, P. I.

AU - Sagar, D. R.

AU - Ashraf, S.

AU - Burston, J. J.

AU - Suri, S.

AU - Chapman, V.

AU - Walsh, D. A.

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N2 - ObjectivesTo characterize differences in joint pathology and pain behavior between two rat models of osteoarthritis (OA) in order to inform selection of animal models for interventional studies.MethodKnee OA was induced in Sprague Dawley rats by either meniscal transection (MNX) or intra-articular injection of monosodium iodoacetate (MIA). Controls were subjected to sham surgery or saline-injection. In a separate experiment, a single intra-articular injection of triamcinolone acetonide was administered 14 days after MNX or MIA arthritis induction. Pain behavior and joint pathology were quantified.ResultsBoth models displayed synovial inflammation, chondropathy and osteophytosis. Chondropathy scores increased with time similarly in the two models. Inflammation and osteophyte scores were greater in MNX model compared to the MIA model. At day 49, the MNX model exhibited a greater number of channels crossing the osteochondral junction compared to all other groups. The MNX model exhibited greater weight bearing asymmetry compared to the MIA model, whereas the MIA model displayed more consistent hindpaw allodynia. Triamcinolone attenuated weight bearing asymmetry and distal allodynia to control levels in the MNX model, but distal allodynia was unaltered in the MIA model.ConclusionsThe comparison of the two models of OA in rats, using identical assessment tools has demonstrated that although both models display features of OA, there are differences between the models which may represent different aspects of human OA. Thus, model selection should be based on the pathological aspects of OA under investigation.

AB - ObjectivesTo characterize differences in joint pathology and pain behavior between two rat models of osteoarthritis (OA) in order to inform selection of animal models for interventional studies.MethodKnee OA was induced in Sprague Dawley rats by either meniscal transection (MNX) or intra-articular injection of monosodium iodoacetate (MIA). Controls were subjected to sham surgery or saline-injection. In a separate experiment, a single intra-articular injection of triamcinolone acetonide was administered 14 days after MNX or MIA arthritis induction. Pain behavior and joint pathology were quantified.ResultsBoth models displayed synovial inflammation, chondropathy and osteophytosis. Chondropathy scores increased with time similarly in the two models. Inflammation and osteophyte scores were greater in MNX model compared to the MIA model. At day 49, the MNX model exhibited a greater number of channels crossing the osteochondral junction compared to all other groups. The MNX model exhibited greater weight bearing asymmetry compared to the MIA model, whereas the MIA model displayed more consistent hindpaw allodynia. Triamcinolone attenuated weight bearing asymmetry and distal allodynia to control levels in the MNX model, but distal allodynia was unaltered in the MIA model.ConclusionsThe comparison of the two models of OA in rats, using identical assessment tools has demonstrated that although both models display features of OA, there are differences between the models which may represent different aspects of human OA. Thus, model selection should be based on the pathological aspects of OA under investigation.

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