Differential distribution and metabolism of arachidonic acid and docosahexaenoic acid by human placental choriocarcinoma (BeWo) cells

J T Crabtree, Margaret Jane Gordon, Fiona Margaret Campbell, Asim K Dutta-Roy

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The time course of incorporation of [C-14]arachidonic acid and [H-3]docosahexaenoic acid into various lipid fractions in placental choriocarcinoma (BeWo) cells was investigated. BeWo cells were found to rapidly incorporate exogenous [C-14]arachidonic acid and [H-3] docosahexaenoic acid into the total cellular lipid pool. The extent of docosahexaenoic acid esterification was more rapid than for arachidonic acid, although this difference abated with time to leave only a small percentage of the fatty acids in their unesterified form. Furthermore, uptake was found to be saturable. In the cellular lipids these fatty acids were mainly esterified into the phospholipid (PL) and the triacyglycerol (TAG) fractions. Smaller amounts were also detected in the diacylglycerol and cholesterol ester fractions. Almost 60% of the total amount of [H-3]Docosahexaenoic acid taken up by the cells was esterified into TAG whereas 37% was in PL fractions. For arachidonic acid the reverse was true, 60% of the total uptake was incorporated into PL fractions whereas less than 35% was in TAG. Marked differences were also found in the distribution of the fatty acids into individual phospholipid classes. The higher incorporation of docosahexaenoic acid and arachidonic acid was found in PC and PE, respectively. The greater cellular uptake of docosahexaenoic acid and its preferential incorporation in TAG suggests that both uptake and transport modes of this fatty acid by the placenta to fetus is different from that of arachidonic acid.

Original languageEnglish
Pages (from-to)191-198
Number of pages8
JournalMolecular & Cellular Biochemistry
Volume185
Issue number1-2
DOIs
Publication statusPublished - Aug 1998

Keywords

  • fatty acid uptake
  • metabolism
  • placenta
  • BeWo cells
  • long chain polyunsaturated fatty acids
  • arachidonic acid
  • docosahexaenoic acid
  • FABP(pm)
  • fatty acid-binding protein
  • BINDING PROTEIN FABP(PM)
  • FATTY-ACID
  • TRANSPORT
  • BRAIN
  • ERYTHROCYTES
  • REQUIREMENTS
  • MEMBRANES
  • fatty acid-binding protein
  • binding protein FABP(PM)
  • fatty-acid
  • transport
  • brain
  • erythrocytes
  • requirements
  • membranes

Cite this

Differential distribution and metabolism of arachidonic acid and docosahexaenoic acid by human placental choriocarcinoma (BeWo) cells. / Crabtree, J T ; Gordon, Margaret Jane; Campbell, Fiona Margaret; Dutta-Roy, Asim K.

In: Molecular & Cellular Biochemistry, Vol. 185, No. 1-2, 08.1998, p. 191-198.

Research output: Contribution to journalArticle

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abstract = "The time course of incorporation of [C-14]arachidonic acid and [H-3]docosahexaenoic acid into various lipid fractions in placental choriocarcinoma (BeWo) cells was investigated. BeWo cells were found to rapidly incorporate exogenous [C-14]arachidonic acid and [H-3] docosahexaenoic acid into the total cellular lipid pool. The extent of docosahexaenoic acid esterification was more rapid than for arachidonic acid, although this difference abated with time to leave only a small percentage of the fatty acids in their unesterified form. Furthermore, uptake was found to be saturable. In the cellular lipids these fatty acids were mainly esterified into the phospholipid (PL) and the triacyglycerol (TAG) fractions. Smaller amounts were also detected in the diacylglycerol and cholesterol ester fractions. Almost 60{\%} of the total amount of [H-3]Docosahexaenoic acid taken up by the cells was esterified into TAG whereas 37{\%} was in PL fractions. For arachidonic acid the reverse was true, 60{\%} of the total uptake was incorporated into PL fractions whereas less than 35{\%} was in TAG. Marked differences were also found in the distribution of the fatty acids into individual phospholipid classes. The higher incorporation of docosahexaenoic acid and arachidonic acid was found in PC and PE, respectively. The greater cellular uptake of docosahexaenoic acid and its preferential incorporation in TAG suggests that both uptake and transport modes of this fatty acid by the placenta to fetus is different from that of arachidonic acid.",
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T1 - Differential distribution and metabolism of arachidonic acid and docosahexaenoic acid by human placental choriocarcinoma (BeWo) cells

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AU - Gordon, Margaret Jane

AU - Campbell, Fiona Margaret

AU - Dutta-Roy, Asim K

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AB - The time course of incorporation of [C-14]arachidonic acid and [H-3]docosahexaenoic acid into various lipid fractions in placental choriocarcinoma (BeWo) cells was investigated. BeWo cells were found to rapidly incorporate exogenous [C-14]arachidonic acid and [H-3] docosahexaenoic acid into the total cellular lipid pool. The extent of docosahexaenoic acid esterification was more rapid than for arachidonic acid, although this difference abated with time to leave only a small percentage of the fatty acids in their unesterified form. Furthermore, uptake was found to be saturable. In the cellular lipids these fatty acids were mainly esterified into the phospholipid (PL) and the triacyglycerol (TAG) fractions. Smaller amounts were also detected in the diacylglycerol and cholesterol ester fractions. Almost 60% of the total amount of [H-3]Docosahexaenoic acid taken up by the cells was esterified into TAG whereas 37% was in PL fractions. For arachidonic acid the reverse was true, 60% of the total uptake was incorporated into PL fractions whereas less than 35% was in TAG. Marked differences were also found in the distribution of the fatty acids into individual phospholipid classes. The higher incorporation of docosahexaenoic acid and arachidonic acid was found in PC and PE, respectively. The greater cellular uptake of docosahexaenoic acid and its preferential incorporation in TAG suggests that both uptake and transport modes of this fatty acid by the placenta to fetus is different from that of arachidonic acid.

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KW - long chain polyunsaturated fatty acids

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KW - TRANSPORT

KW - BRAIN

KW - ERYTHROCYTES

KW - REQUIREMENTS

KW - MEMBRANES

KW - fatty acid-binding protein

KW - binding protein FABP(PM)

KW - fatty-acid

KW - transport

KW - brain

KW - erythrocytes

KW - requirements

KW - membranes

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DO - 10.1023/A:1006852230337

M3 - Article

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SP - 191

EP - 198

JO - Molecular & Cellular Biochemistry

JF - Molecular & Cellular Biochemistry

SN - 0300-8177

IS - 1-2

ER -