Differential Effects of Doses and Forms of Dietary Selenium on Immune Cell Numbers in the Skin of Ultraviolet-irradiated and Unirradiated Mice

Roderick C McKenzie, Geoff J. Beckett, Steven McLean, John Arthur, Joanna C. Macve, Fergus Nicol, Forbes Howie, Mary Norval

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The effect of three different doses of dietary L-selenomethionine (SM) and sodium selenite (SS) on skin selenium (Se) content, glutathione peroxidase (GPx) activity, Langerhans cell (LC) and mast cell numbers in ultraviolet radiation-B (UVB)-irradiated and unirradiated C3H/HeN mice was determined. After weaning, groups of mice were given Se-deficient, Se-adequate, or Se-high diets. Six weeks later, some animals in each group were exposed to a single UVB dose (acute), while others were exposed three times weekly for the following 40 weeks (chronic). The skin Se content and GPx activity increased in all the Se-supplemented groups, and the latter was not altered by UVB exposure. Generally, the Se-containing diets caused an increase in LC numbers at 6 weeks and a further rise at 40 weeks, but did not prevent the loss induced by acute or chronic UVB radiation. Skin mast cell numbers were highest in animals fed the Se-deficient diet after 6 and 40 weeks. Acute and chronic UVB radiation decreased the mast cell number and dietary Se did not prevent the reduction. While the present study shows that Se plays an important role in governing the number of LCs and mast cells in the skin, no protective effect against the immunomodulating properties of UVB radiation on these cell types was observed. However, this conclusion may only apply to the experimental conditions chosen, and additional studies at different Se dosages and reduced intensities of chronic UVB exposure are required to confirm the results.

Original languageEnglish
Pages (from-to)255-267
Number of pages13
JournalBiological Trace Element Research
Volume125
Issue number3
Early online date24 Jun 2008
DOIs
Publication statusPublished - Dec 2008

Keywords

  • Langerhans cells
  • selenium
  • mast cells
  • glutathione peroxidase
  • ultraviolet radiation
  • glutathione-peroxidase
  • cancer prevention
  • radiation
  • supplementation
  • expression
  • carcinoma
  • humans
  • death
  • Langerhans cells
  • Selenium
  • Mast cells
  • Glutathione peroxidase
  • Ultraviolet radiation

Cite this

Differential Effects of Doses and Forms of Dietary Selenium on Immune Cell Numbers in the Skin of Ultraviolet-irradiated and Unirradiated Mice. / McKenzie, Roderick C; Beckett, Geoff J.; McLean, Steven; Arthur, John; Macve, Joanna C.; Nicol, Fergus; Howie, Forbes; Norval, Mary.

In: Biological Trace Element Research, Vol. 125, No. 3, 12.2008, p. 255-267.

Research output: Contribution to journalArticle

McKenzie, Roderick C ; Beckett, Geoff J. ; McLean, Steven ; Arthur, John ; Macve, Joanna C. ; Nicol, Fergus ; Howie, Forbes ; Norval, Mary. / Differential Effects of Doses and Forms of Dietary Selenium on Immune Cell Numbers in the Skin of Ultraviolet-irradiated and Unirradiated Mice. In: Biological Trace Element Research. 2008 ; Vol. 125, No. 3. pp. 255-267.
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abstract = "The effect of three different doses of dietary L-selenomethionine (SM) and sodium selenite (SS) on skin selenium (Se) content, glutathione peroxidase (GPx) activity, Langerhans cell (LC) and mast cell numbers in ultraviolet radiation-B (UVB)-irradiated and unirradiated C3H/HeN mice was determined. After weaning, groups of mice were given Se-deficient, Se-adequate, or Se-high diets. Six weeks later, some animals in each group were exposed to a single UVB dose (acute), while others were exposed three times weekly for the following 40 weeks (chronic). The skin Se content and GPx activity increased in all the Se-supplemented groups, and the latter was not altered by UVB exposure. Generally, the Se-containing diets caused an increase in LC numbers at 6 weeks and a further rise at 40 weeks, but did not prevent the loss induced by acute or chronic UVB radiation. Skin mast cell numbers were highest in animals fed the Se-deficient diet after 6 and 40 weeks. Acute and chronic UVB radiation decreased the mast cell number and dietary Se did not prevent the reduction. While the present study shows that Se plays an important role in governing the number of LCs and mast cells in the skin, no protective effect against the immunomodulating properties of UVB radiation on these cell types was observed. However, this conclusion may only apply to the experimental conditions chosen, and additional studies at different Se dosages and reduced intensities of chronic UVB exposure are required to confirm the results.",
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T1 - Differential Effects of Doses and Forms of Dietary Selenium on Immune Cell Numbers in the Skin of Ultraviolet-irradiated and Unirradiated Mice

AU - McKenzie, Roderick C

AU - Beckett, Geoff J.

AU - McLean, Steven

AU - Arthur, John

AU - Macve, Joanna C.

AU - Nicol, Fergus

AU - Howie, Forbes

AU - Norval, Mary

PY - 2008/12

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N2 - The effect of three different doses of dietary L-selenomethionine (SM) and sodium selenite (SS) on skin selenium (Se) content, glutathione peroxidase (GPx) activity, Langerhans cell (LC) and mast cell numbers in ultraviolet radiation-B (UVB)-irradiated and unirradiated C3H/HeN mice was determined. After weaning, groups of mice were given Se-deficient, Se-adequate, or Se-high diets. Six weeks later, some animals in each group were exposed to a single UVB dose (acute), while others were exposed three times weekly for the following 40 weeks (chronic). The skin Se content and GPx activity increased in all the Se-supplemented groups, and the latter was not altered by UVB exposure. Generally, the Se-containing diets caused an increase in LC numbers at 6 weeks and a further rise at 40 weeks, but did not prevent the loss induced by acute or chronic UVB radiation. Skin mast cell numbers were highest in animals fed the Se-deficient diet after 6 and 40 weeks. Acute and chronic UVB radiation decreased the mast cell number and dietary Se did not prevent the reduction. While the present study shows that Se plays an important role in governing the number of LCs and mast cells in the skin, no protective effect against the immunomodulating properties of UVB radiation on these cell types was observed. However, this conclusion may only apply to the experimental conditions chosen, and additional studies at different Se dosages and reduced intensities of chronic UVB exposure are required to confirm the results.

AB - The effect of three different doses of dietary L-selenomethionine (SM) and sodium selenite (SS) on skin selenium (Se) content, glutathione peroxidase (GPx) activity, Langerhans cell (LC) and mast cell numbers in ultraviolet radiation-B (UVB)-irradiated and unirradiated C3H/HeN mice was determined. After weaning, groups of mice were given Se-deficient, Se-adequate, or Se-high diets. Six weeks later, some animals in each group were exposed to a single UVB dose (acute), while others were exposed three times weekly for the following 40 weeks (chronic). The skin Se content and GPx activity increased in all the Se-supplemented groups, and the latter was not altered by UVB exposure. Generally, the Se-containing diets caused an increase in LC numbers at 6 weeks and a further rise at 40 weeks, but did not prevent the loss induced by acute or chronic UVB radiation. Skin mast cell numbers were highest in animals fed the Se-deficient diet after 6 and 40 weeks. Acute and chronic UVB radiation decreased the mast cell number and dietary Se did not prevent the reduction. While the present study shows that Se plays an important role in governing the number of LCs and mast cells in the skin, no protective effect against the immunomodulating properties of UVB radiation on these cell types was observed. However, this conclusion may only apply to the experimental conditions chosen, and additional studies at different Se dosages and reduced intensities of chronic UVB exposure are required to confirm the results.

KW - Langerhans cells

KW - selenium

KW - mast cells

KW - glutathione peroxidase

KW - ultraviolet radiation

KW - glutathione-peroxidase

KW - cancer prevention

KW - radiation

KW - supplementation

KW - expression

KW - carcinoma

KW - humans

KW - death

KW - Langerhans cells

KW - Selenium

KW - Mast cells

KW - Glutathione peroxidase

KW - Ultraviolet radiation

U2 - 10.1007/s12011-008-8171-2

DO - 10.1007/s12011-008-8171-2

M3 - Article

VL - 125

SP - 255

EP - 267

JO - Biological Trace Element Research

JF - Biological Trace Element Research

SN - 0163-4984

IS - 3

ER -