Differential effects of microorganism-invertebrate interactions on benthic nitrogen cycling

William W. Gilbertson, Martin Solan, James I. Prosser

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Infaunal invertebrate activity can fundamentally alter physicochemical conditions in sediments and influence nutrient cycling. However, despite clear links between invertebrate activity and microbially mediated processes such as nitrification, the mechanisms by which bioturbating macrofauna affect microbial communities have received little attention. This study provides strong evidence for differential stimulation of microbial nitrogen transformations by three functionally contrasting species of macrofauna (Hediste diversicolor, Corophium volutator, Hydrobia ulvae). Despite increased nitrification, abundance of ammonia-oxidising bacteria (AOB) and ammonia-oxidising archaea (AOA) at the sedimentwater interface did not significantly change in the presence of macrofauna. However, species-specific differences in macrofaunal activity did influence ammonia oxidiser community structure, increasing AOB abundance relative to AOA in the presence of C. volutator or H. ulvae, but with no change in H. diversicolor and no-macrofauna treatments. Denaturing gradient gel electrophoresis profiles were similar between macrofaunal treatments, although one AOB band increased in relative intensity in the presence of C. volutator, decreased in the H. diversicolor treatment and was unchanged in the H. ulvae treatment. These data suggest that links between bioturbating macrofauna and nutrient cycling are not expressed through changes in the abundance of ammonia oxidisers in surface sediments, but are associated with changes in the AOA : AOB ratio depending on the invertebrate species.

Original languageEnglish
Pages (from-to)11-22
Number of pages12
JournalFEMS Microbiology Ecology
Volume82
Issue number1
Early online date15 May 2012
DOIs
Publication statusPublished - Oct 2012

Keywords

  • ammonia oxidisers
  • bioturbation
  • nitrification
  • amoA
  • ecosystem function
  • nutrient cycling

Cite this

Differential effects of microorganism-invertebrate interactions on benthic nitrogen cycling. / Gilbertson, William W.; Solan, Martin; Prosser, James I.

In: FEMS Microbiology Ecology, Vol. 82, No. 1, 10.2012, p. 11-22.

Research output: Contribution to journalArticle

@article{e1d6a9a51ffe463bad66d65459ce05f5,
title = "Differential effects of microorganism-invertebrate interactions on benthic nitrogen cycling",
abstract = "Infaunal invertebrate activity can fundamentally alter physicochemical conditions in sediments and influence nutrient cycling. However, despite clear links between invertebrate activity and microbially mediated processes such as nitrification, the mechanisms by which bioturbating macrofauna affect microbial communities have received little attention. This study provides strong evidence for differential stimulation of microbial nitrogen transformations by three functionally contrasting species of macrofauna (Hediste diversicolor, Corophium volutator, Hydrobia ulvae). Despite increased nitrification, abundance of ammonia-oxidising bacteria (AOB) and ammonia-oxidising archaea (AOA) at the sedimentwater interface did not significantly change in the presence of macrofauna. However, species-specific differences in macrofaunal activity did influence ammonia oxidiser community structure, increasing AOB abundance relative to AOA in the presence of C. volutator or H. ulvae, but with no change in H. diversicolor and no-macrofauna treatments. Denaturing gradient gel electrophoresis profiles were similar between macrofaunal treatments, although one AOB band increased in relative intensity in the presence of C. volutator, decreased in the H. diversicolor treatment and was unchanged in the H. ulvae treatment. These data suggest that links between bioturbating macrofauna and nutrient cycling are not expressed through changes in the abundance of ammonia oxidisers in surface sediments, but are associated with changes in the AOA : AOB ratio depending on the invertebrate species.",
keywords = "ammonia oxidisers, bioturbation, nitrification, amoA, ecosystem function, nutrient cycling",
author = "Gilbertson, {William W.} and Martin Solan and Prosser, {James I.}",
year = "2012",
month = "10",
doi = "10.1111/j.1574-6941.2012.01400.x",
language = "English",
volume = "82",
pages = "11--22",
journal = "FEMS Microbiology Ecology",
issn = "1574-6941",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Differential effects of microorganism-invertebrate interactions on benthic nitrogen cycling

AU - Gilbertson, William W.

AU - Solan, Martin

AU - Prosser, James I.

PY - 2012/10

Y1 - 2012/10

N2 - Infaunal invertebrate activity can fundamentally alter physicochemical conditions in sediments and influence nutrient cycling. However, despite clear links between invertebrate activity and microbially mediated processes such as nitrification, the mechanisms by which bioturbating macrofauna affect microbial communities have received little attention. This study provides strong evidence for differential stimulation of microbial nitrogen transformations by three functionally contrasting species of macrofauna (Hediste diversicolor, Corophium volutator, Hydrobia ulvae). Despite increased nitrification, abundance of ammonia-oxidising bacteria (AOB) and ammonia-oxidising archaea (AOA) at the sedimentwater interface did not significantly change in the presence of macrofauna. However, species-specific differences in macrofaunal activity did influence ammonia oxidiser community structure, increasing AOB abundance relative to AOA in the presence of C. volutator or H. ulvae, but with no change in H. diversicolor and no-macrofauna treatments. Denaturing gradient gel electrophoresis profiles were similar between macrofaunal treatments, although one AOB band increased in relative intensity in the presence of C. volutator, decreased in the H. diversicolor treatment and was unchanged in the H. ulvae treatment. These data suggest that links between bioturbating macrofauna and nutrient cycling are not expressed through changes in the abundance of ammonia oxidisers in surface sediments, but are associated with changes in the AOA : AOB ratio depending on the invertebrate species.

AB - Infaunal invertebrate activity can fundamentally alter physicochemical conditions in sediments and influence nutrient cycling. However, despite clear links between invertebrate activity and microbially mediated processes such as nitrification, the mechanisms by which bioturbating macrofauna affect microbial communities have received little attention. This study provides strong evidence for differential stimulation of microbial nitrogen transformations by three functionally contrasting species of macrofauna (Hediste diversicolor, Corophium volutator, Hydrobia ulvae). Despite increased nitrification, abundance of ammonia-oxidising bacteria (AOB) and ammonia-oxidising archaea (AOA) at the sedimentwater interface did not significantly change in the presence of macrofauna. However, species-specific differences in macrofaunal activity did influence ammonia oxidiser community structure, increasing AOB abundance relative to AOA in the presence of C. volutator or H. ulvae, but with no change in H. diversicolor and no-macrofauna treatments. Denaturing gradient gel electrophoresis profiles were similar between macrofaunal treatments, although one AOB band increased in relative intensity in the presence of C. volutator, decreased in the H. diversicolor treatment and was unchanged in the H. ulvae treatment. These data suggest that links between bioturbating macrofauna and nutrient cycling are not expressed through changes in the abundance of ammonia oxidisers in surface sediments, but are associated with changes in the AOA : AOB ratio depending on the invertebrate species.

KW - ammonia oxidisers

KW - bioturbation

KW - nitrification

KW - amoA

KW - ecosystem function

KW - nutrient cycling

U2 - 10.1111/j.1574-6941.2012.01400.x

DO - 10.1111/j.1574-6941.2012.01400.x

M3 - Article

VL - 82

SP - 11

EP - 22

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 1574-6941

IS - 1

ER -