Differential expression and haplotypic variation of two interleukin-1b genes in the common carp (Cyprinus carpio L.).

M. Engelsma, Rene Josephus Maria Stet, J. P. J. Saeij, B. M. L. Verburg-van Kemenade

    Research output: Contribution to journalArticle

    60 Citations (Scopus)

    Abstract

    Interleukin-1beta (Il-1beta) is a central component in innate immunity and the inflammatory response of mammals. Only recently, the first non-mammalian IL-1beta sequences were published. In this study, we describe a second IL-1beta sequence (IL-1beta2) in carp with 74% amino acid identity to the carp IL-1beta1 sequence. The existence of two IL-1beta copies in the carp genome probably originates from the tetraploid nature of the species. In contrast to the first carp Il-1beta sequence, IL-1beta2 is represented by multiple genes with 95-99% identity. Detection of several IL-1beta2 sequences within individual homozygous fish suggests the presence of multiple copies of the Il-1beta2 gene in the carp genome, possibly as a result of subsequent gene duplication of IL-1beta2. In vivo, constitutive mRNA expression of both IL-1beta genes was found in healthy carp. IL-1beta2 mRNA expression could be up-regulated in head kidney cells similar to carp IL-1beta1, in vivo by infection with Trypanoplasma borreli and in vitro by stimulation with lipopolysaccharide (LPS). Cortisol, the major glucocorticoid in fish, is an endocrine-derived factor mediating Il-1beta expression. Although constitutive IL-1beta expression was inhibited by a physiological dose of cortisol, cortisol synergistically enhanced LPS-induced IL-1beta expression in carp. Involvement of the transcription factor nuclear factor (NF)-kappaB in expression of IL-1beta1 and IL-1beta2 was demonstrated. Ratio of IL-1beta expression was determined and this showed IL-1beta1 mRNA expression to be at least tenfold higher compared with IL-1beta2. The possibilities of IL-1beta2 being a functional gene or approaching pseudogene status are discussed. (C) 2002 Elsevier Science Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)21-31
    Number of pages10
    JournalCytokine
    Volume22
    DOIs
    Publication statusPublished - 2003

    Keywords

    • Cyprinus carpio
    • expression
    • fish
    • interleukin-1
    • tetraploidy
    • TROUT ONCORHYNCHUS-MYKISS
    • MOLECULAR-CLONING
    • BIOLOGICAL-ACTIVITY
    • NITRIC-OXIDE
    • IL-1-BETA
    • FISH
    • MEMBERS
    • POLYMORPHISM
    • DISEASE
    • FAMILY

    Cite this

    Differential expression and haplotypic variation of two interleukin-1b genes in the common carp (Cyprinus carpio L.). / Engelsma, M.; Stet, Rene Josephus Maria; Saeij, J. P. J.; Verburg-van Kemenade, B. M. L.

    In: Cytokine, Vol. 22, 2003, p. 21-31.

    Research output: Contribution to journalArticle

    Engelsma, M. ; Stet, Rene Josephus Maria ; Saeij, J. P. J. ; Verburg-van Kemenade, B. M. L. / Differential expression and haplotypic variation of two interleukin-1b genes in the common carp (Cyprinus carpio L.). In: Cytokine. 2003 ; Vol. 22. pp. 21-31.
    @article{0e22b3afda3a4d71aaed04d446074e5f,
    title = "Differential expression and haplotypic variation of two interleukin-1b genes in the common carp (Cyprinus carpio L.).",
    abstract = "Interleukin-1beta (Il-1beta) is a central component in innate immunity and the inflammatory response of mammals. Only recently, the first non-mammalian IL-1beta sequences were published. In this study, we describe a second IL-1beta sequence (IL-1beta2) in carp with 74{\%} amino acid identity to the carp IL-1beta1 sequence. The existence of two IL-1beta copies in the carp genome probably originates from the tetraploid nature of the species. In contrast to the first carp Il-1beta sequence, IL-1beta2 is represented by multiple genes with 95-99{\%} identity. Detection of several IL-1beta2 sequences within individual homozygous fish suggests the presence of multiple copies of the Il-1beta2 gene in the carp genome, possibly as a result of subsequent gene duplication of IL-1beta2. In vivo, constitutive mRNA expression of both IL-1beta genes was found in healthy carp. IL-1beta2 mRNA expression could be up-regulated in head kidney cells similar to carp IL-1beta1, in vivo by infection with Trypanoplasma borreli and in vitro by stimulation with lipopolysaccharide (LPS). Cortisol, the major glucocorticoid in fish, is an endocrine-derived factor mediating Il-1beta expression. Although constitutive IL-1beta expression was inhibited by a physiological dose of cortisol, cortisol synergistically enhanced LPS-induced IL-1beta expression in carp. Involvement of the transcription factor nuclear factor (NF)-kappaB in expression of IL-1beta1 and IL-1beta2 was demonstrated. Ratio of IL-1beta expression was determined and this showed IL-1beta1 mRNA expression to be at least tenfold higher compared with IL-1beta2. The possibilities of IL-1beta2 being a functional gene or approaching pseudogene status are discussed. (C) 2002 Elsevier Science Ltd. All rights reserved.",
    keywords = "Cyprinus carpio, expression, fish, interleukin-1, tetraploidy, TROUT ONCORHYNCHUS-MYKISS, MOLECULAR-CLONING, BIOLOGICAL-ACTIVITY, NITRIC-OXIDE, IL-1-BETA, FISH, MEMBERS, POLYMORPHISM, DISEASE, FAMILY",
    author = "M. Engelsma and Stet, {Rene Josephus Maria} and Saeij, {J. P. J.} and {Verburg-van Kemenade}, {B. M. L.}",
    year = "2003",
    doi = "10.1016/S1043-4666(03)00102-9",
    language = "English",
    volume = "22",
    pages = "21--31",
    journal = "Cytokine",
    issn = "1043-4666",
    publisher = "Academic Press Inc.",

    }

    TY - JOUR

    T1 - Differential expression and haplotypic variation of two interleukin-1b genes in the common carp (Cyprinus carpio L.).

    AU - Engelsma, M.

    AU - Stet, Rene Josephus Maria

    AU - Saeij, J. P. J.

    AU - Verburg-van Kemenade, B. M. L.

    PY - 2003

    Y1 - 2003

    N2 - Interleukin-1beta (Il-1beta) is a central component in innate immunity and the inflammatory response of mammals. Only recently, the first non-mammalian IL-1beta sequences were published. In this study, we describe a second IL-1beta sequence (IL-1beta2) in carp with 74% amino acid identity to the carp IL-1beta1 sequence. The existence of two IL-1beta copies in the carp genome probably originates from the tetraploid nature of the species. In contrast to the first carp Il-1beta sequence, IL-1beta2 is represented by multiple genes with 95-99% identity. Detection of several IL-1beta2 sequences within individual homozygous fish suggests the presence of multiple copies of the Il-1beta2 gene in the carp genome, possibly as a result of subsequent gene duplication of IL-1beta2. In vivo, constitutive mRNA expression of both IL-1beta genes was found in healthy carp. IL-1beta2 mRNA expression could be up-regulated in head kidney cells similar to carp IL-1beta1, in vivo by infection with Trypanoplasma borreli and in vitro by stimulation with lipopolysaccharide (LPS). Cortisol, the major glucocorticoid in fish, is an endocrine-derived factor mediating Il-1beta expression. Although constitutive IL-1beta expression was inhibited by a physiological dose of cortisol, cortisol synergistically enhanced LPS-induced IL-1beta expression in carp. Involvement of the transcription factor nuclear factor (NF)-kappaB in expression of IL-1beta1 and IL-1beta2 was demonstrated. Ratio of IL-1beta expression was determined and this showed IL-1beta1 mRNA expression to be at least tenfold higher compared with IL-1beta2. The possibilities of IL-1beta2 being a functional gene or approaching pseudogene status are discussed. (C) 2002 Elsevier Science Ltd. All rights reserved.

    AB - Interleukin-1beta (Il-1beta) is a central component in innate immunity and the inflammatory response of mammals. Only recently, the first non-mammalian IL-1beta sequences were published. In this study, we describe a second IL-1beta sequence (IL-1beta2) in carp with 74% amino acid identity to the carp IL-1beta1 sequence. The existence of two IL-1beta copies in the carp genome probably originates from the tetraploid nature of the species. In contrast to the first carp Il-1beta sequence, IL-1beta2 is represented by multiple genes with 95-99% identity. Detection of several IL-1beta2 sequences within individual homozygous fish suggests the presence of multiple copies of the Il-1beta2 gene in the carp genome, possibly as a result of subsequent gene duplication of IL-1beta2. In vivo, constitutive mRNA expression of both IL-1beta genes was found in healthy carp. IL-1beta2 mRNA expression could be up-regulated in head kidney cells similar to carp IL-1beta1, in vivo by infection with Trypanoplasma borreli and in vitro by stimulation with lipopolysaccharide (LPS). Cortisol, the major glucocorticoid in fish, is an endocrine-derived factor mediating Il-1beta expression. Although constitutive IL-1beta expression was inhibited by a physiological dose of cortisol, cortisol synergistically enhanced LPS-induced IL-1beta expression in carp. Involvement of the transcription factor nuclear factor (NF)-kappaB in expression of IL-1beta1 and IL-1beta2 was demonstrated. Ratio of IL-1beta expression was determined and this showed IL-1beta1 mRNA expression to be at least tenfold higher compared with IL-1beta2. The possibilities of IL-1beta2 being a functional gene or approaching pseudogene status are discussed. (C) 2002 Elsevier Science Ltd. All rights reserved.

    KW - Cyprinus carpio

    KW - expression

    KW - fish

    KW - interleukin-1

    KW - tetraploidy

    KW - TROUT ONCORHYNCHUS-MYKISS

    KW - MOLECULAR-CLONING

    KW - BIOLOGICAL-ACTIVITY

    KW - NITRIC-OXIDE

    KW - IL-1-BETA

    KW - FISH

    KW - MEMBERS

    KW - POLYMORPHISM

    KW - DISEASE

    KW - FAMILY

    U2 - 10.1016/S1043-4666(03)00102-9

    DO - 10.1016/S1043-4666(03)00102-9

    M3 - Article

    VL - 22

    SP - 21

    EP - 31

    JO - Cytokine

    JF - Cytokine

    SN - 1043-4666

    ER -