Abstract
To study the regulation of the thyroid system, an Atlantic salmon Salmo salar cDNA clone was isolated for thyroid stimulating hormone (TSH) beta subunit gene. A cDNA (866 bp) was isolated from an adult Atlantic salmon pituitary cDNA library, this clone was sequenced and shown to be highly conserved when compared to other teleost beta TSH subunit sequences. The cDNA was used as a probe for Northern blot analysis of total pituitary RNA from the different life cycle stages of Atlantic salmon. Northern blot analysis demonstrated that beta TSH mRNA is expressed at all life cycle stages studied, including parr, smelt, immature fish at sea and sexually mature male fish. Densitometry of Northern blots showed that sexually mature male salmon had low levels of salmon beta TSH mRNA compared to non-mature fish. Stunts, fish performing poorly in salt water, were shown to have elevated levels of beta TSH mRNA when compared to healthy fish. (C) 1999 The Fisheries Society of the British Isles.
| Original language | English |
|---|---|
| Pages (from-to) | 757-766 |
| Number of pages | 10 |
| Journal | Journal of Fish Biology |
| Volume | 54 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 1999 |
Keywords
- Atlantic salmon
- Salmo salar
- thyrotropin beta subunit
- gene expression
- cDNA sequence
- Parr-Smolt transformation
- plasma thyroid hormones
- messenger-RNA levels
- growth-hormone
- coho salmon
- steelhead trout
- chinook salmon
- teleost fish
- salar L
- smoltification
Cite this
Differential expression of Atlantic salmon thyrotropin beta subunit mRNA and its cDNA sequence. / Martin, Samuel Allen Moore; Wallner, W ; Youngson, A F ; Smith, T .
In: Journal of Fish Biology, Vol. 54, No. 4, 04.1999, p. 757-766.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential expression of Atlantic salmon thyrotropin beta subunit mRNA and its cDNA sequence
AU - Martin, Samuel Allen Moore
AU - Wallner, W
AU - Youngson, A F
AU - Smith, T
PY - 1999/4
Y1 - 1999/4
N2 - To study the regulation of the thyroid system, an Atlantic salmon Salmo salar cDNA clone was isolated for thyroid stimulating hormone (TSH) beta subunit gene. A cDNA (866 bp) was isolated from an adult Atlantic salmon pituitary cDNA library, this clone was sequenced and shown to be highly conserved when compared to other teleost beta TSH subunit sequences. The cDNA was used as a probe for Northern blot analysis of total pituitary RNA from the different life cycle stages of Atlantic salmon. Northern blot analysis demonstrated that beta TSH mRNA is expressed at all life cycle stages studied, including parr, smelt, immature fish at sea and sexually mature male fish. Densitometry of Northern blots showed that sexually mature male salmon had low levels of salmon beta TSH mRNA compared to non-mature fish. Stunts, fish performing poorly in salt water, were shown to have elevated levels of beta TSH mRNA when compared to healthy fish. (C) 1999 The Fisheries Society of the British Isles.
AB - To study the regulation of the thyroid system, an Atlantic salmon Salmo salar cDNA clone was isolated for thyroid stimulating hormone (TSH) beta subunit gene. A cDNA (866 bp) was isolated from an adult Atlantic salmon pituitary cDNA library, this clone was sequenced and shown to be highly conserved when compared to other teleost beta TSH subunit sequences. The cDNA was used as a probe for Northern blot analysis of total pituitary RNA from the different life cycle stages of Atlantic salmon. Northern blot analysis demonstrated that beta TSH mRNA is expressed at all life cycle stages studied, including parr, smelt, immature fish at sea and sexually mature male fish. Densitometry of Northern blots showed that sexually mature male salmon had low levels of salmon beta TSH mRNA compared to non-mature fish. Stunts, fish performing poorly in salt water, were shown to have elevated levels of beta TSH mRNA when compared to healthy fish. (C) 1999 The Fisheries Society of the British Isles.
KW - Atlantic salmon
KW - Salmo salar
KW - thyrotropin beta subunit
KW - gene expression
KW - cDNA sequence
KW - Parr-Smolt transformation
KW - plasma thyroid hormones
KW - messenger-RNA levels
KW - growth-hormone
KW - coho salmon
KW - steelhead trout
KW - chinook salmon
KW - teleost fish
KW - salar L
KW - smoltification
U2 - 10.1111/j.1095-8649.1999.tb02031.x
DO - 10.1111/j.1095-8649.1999.tb02031.x
M3 - Article
VL - 54
SP - 757
EP - 766
JO - Journal of Fish Biology
JF - Journal of Fish Biology
SN - 0022-1112
IS - 4
ER -