Differential expression of leukocyte receptor complex-encoded Ig-like receptors correlates with the transition from effector to memory CTL

Neil Thomas Young, M Uhrberg, J H Phillips, L L Lanier, P Parham

Research output: Contribution to journalArticlepeer-review

147 Citations (Scopus)

Abstract

The human leukocyte receptor complex (LRC) on chromosome 19q13.4 encodes Ig superfamily receptors expressed on hemopoietic cells. Killer Ig-like receptors (KIR) are expressed in cytotoxic lymphocytes but other LRC molecules (Ig-like transcript(ILT)/leukocyte Ig-like receptor (LIR)) are more ubiquitous. We investigated expression of the ILT2/LIR1 inhibitory receptor compared with the related KIR. Both ILT2/LIR1 and KIR were expressed by peripheral CD8(+) T cells with a memory/effector phenotype. ILT2/LIR1(+) T cells demonstrated diverse TCRBV repertoires in contrast to KIR(+) T cells, while numbers of peripheral ILT2/LIR1(+) T cells were greater than KIR(+) T cells and the majority of ILT2/LIR1(+) T cells did not coexpress KIR. Analysis of CD8(+) T cells with specific HLA class I tetramers confirmed this pattern of expression, indicating differential regulation of LRC gene expression in T lymphocytes. Only a minor proportion of ILT2/LIR1(+) KIR(-) clones survived in vitro cloning, were more susceptible to anti-CD3 or cognate peptide induced cell death than KIR(+) T cells and exhibited lower levels of the Bcl-2 survival molecule. Our results indicate a sequential program of LRC-encoded receptor expression with initial ILT2/LIR1 expression in effector T cells and KIR gene transcription in the minor proportion of expanded clones which survives activation-induced cell death to become long term memory T cells.
Original languageEnglish
Pages (from-to)3933-3941
Number of pages9
JournalThe Journal of Immunology
Volume166
Issue number6
Publication statusPublished - 15 Mar 2001

Keywords

  • Antigens, CD
  • Cell Death
  • Cell Line, Transformed
  • Clone Cells
  • Flow Cytometry
  • Humans
  • Immunoglobulins
  • Immunologic Memory
  • Lymphocyte Activation
  • Lymphocyte Subsets
  • Multigene Family
  • Receptors, Antigen, T-Cell, alpha-beta
  • Receptors, Immunologic
  • Receptors, KIR
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocyte Subsets
  • T-Lymphocytes, Cytotoxic

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