Differential expression of leukocyte receptor complex-encoded Ig-like receptors correlates with the transition from effector to memory CTL

Neil Thomas Young, M Uhrberg, J H Phillips, L L Lanier, P Parham

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

The human leukocyte receptor complex (LRC) on chromosome 19q13.4 encodes Ig superfamily receptors expressed on hemopoietic cells. Killer Ig-like receptors (KIR) are expressed in cytotoxic lymphocytes but other LRC molecules (Ig-like transcript(ILT)/leukocyte Ig-like receptor (LIR)) are more ubiquitous. We investigated expression of the ILT2/LIR1 inhibitory receptor compared with the related KIR. Both ILT2/LIR1 and KIR were expressed by peripheral CD8(+) T cells with a memory/effector phenotype. ILT2/LIR1(+) T cells demonstrated diverse TCRBV repertoires in contrast to KIR(+) T cells, while numbers of peripheral ILT2/LIR1(+) T cells were greater than KIR(+) T cells and the majority of ILT2/LIR1(+) T cells did not coexpress KIR. Analysis of CD8(+) T cells with specific HLA class I tetramers confirmed this pattern of expression, indicating differential regulation of LRC gene expression in T lymphocytes. Only a minor proportion of ILT2/LIR1(+) KIR(-) clones survived in vitro cloning, were more susceptible to anti-CD3 or cognate peptide induced cell death than KIR(+) T cells and exhibited lower levels of the Bcl-2 survival molecule. Our results indicate a sequential program of LRC-encoded receptor expression with initial ILT2/LIR1 expression in effector T cells and KIR gene transcription in the minor proportion of expanded clones which survives activation-induced cell death to become long term memory T cells.
Original languageEnglish
Pages (from-to)3933-3941
Number of pages9
JournalThe Journal of Immunology
Volume166
Issue number6
Publication statusPublished - 15 Mar 2001

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Leukocytes
T-Lymphocytes
T-Cell Antigen Receptor
Cell Death
Clone Cells
Long-Term Memory
Organism Cloning
Cell Count
Chromosomes
Lymphocytes
Phenotype
Gene Expression
Peptides
Survival
Genes

Keywords

  • Antigens, CD
  • Cell Death
  • Cell Line, Transformed
  • Clone Cells
  • Flow Cytometry
  • Humans
  • Immunoglobulins
  • Immunologic Memory
  • Lymphocyte Activation
  • Lymphocyte Subsets
  • Multigene Family
  • Receptors, Antigen, T-Cell, alpha-beta
  • Receptors, Immunologic
  • Receptors, KIR
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocyte Subsets
  • T-Lymphocytes, Cytotoxic

Cite this

Differential expression of leukocyte receptor complex-encoded Ig-like receptors correlates with the transition from effector to memory CTL. / Young, Neil Thomas; Uhrberg, M; Phillips, J H; Lanier, L L; Parham, P.

In: The Journal of Immunology, Vol. 166, No. 6, 15.03.2001, p. 3933-3941.

Research output: Contribution to journalArticle

Young, Neil Thomas ; Uhrberg, M ; Phillips, J H ; Lanier, L L ; Parham, P. / Differential expression of leukocyte receptor complex-encoded Ig-like receptors correlates with the transition from effector to memory CTL. In: The Journal of Immunology. 2001 ; Vol. 166, No. 6. pp. 3933-3941.
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AU - Young, Neil Thomas

AU - Uhrberg, M

AU - Phillips, J H

AU - Lanier, L L

AU - Parham, P

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AB - The human leukocyte receptor complex (LRC) on chromosome 19q13.4 encodes Ig superfamily receptors expressed on hemopoietic cells. Killer Ig-like receptors (KIR) are expressed in cytotoxic lymphocytes but other LRC molecules (Ig-like transcript(ILT)/leukocyte Ig-like receptor (LIR)) are more ubiquitous. We investigated expression of the ILT2/LIR1 inhibitory receptor compared with the related KIR. Both ILT2/LIR1 and KIR were expressed by peripheral CD8(+) T cells with a memory/effector phenotype. ILT2/LIR1(+) T cells demonstrated diverse TCRBV repertoires in contrast to KIR(+) T cells, while numbers of peripheral ILT2/LIR1(+) T cells were greater than KIR(+) T cells and the majority of ILT2/LIR1(+) T cells did not coexpress KIR. Analysis of CD8(+) T cells with specific HLA class I tetramers confirmed this pattern of expression, indicating differential regulation of LRC gene expression in T lymphocytes. Only a minor proportion of ILT2/LIR1(+) KIR(-) clones survived in vitro cloning, were more susceptible to anti-CD3 or cognate peptide induced cell death than KIR(+) T cells and exhibited lower levels of the Bcl-2 survival molecule. Our results indicate a sequential program of LRC-encoded receptor expression with initial ILT2/LIR1 expression in effector T cells and KIR gene transcription in the minor proportion of expanded clones which survives activation-induced cell death to become long term memory T cells.

KW - Antigens, CD

KW - Cell Death

KW - Cell Line, Transformed

KW - Clone Cells

KW - Flow Cytometry

KW - Humans

KW - Immunoglobulins

KW - Immunologic Memory

KW - Lymphocyte Activation

KW - Lymphocyte Subsets

KW - Multigene Family

KW - Receptors, Antigen, T-Cell, alpha-beta

KW - Receptors, Immunologic

KW - Receptors, KIR

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - T-Lymphocyte Subsets

KW - T-Lymphocytes, Cytotoxic

M3 - Article

VL - 166

SP - 3933

EP - 3941

JO - The Journal of Immunology

JF - The Journal of Immunology

SN - 0022-1767

IS - 6

ER -