Differential Expression of MicroRNAs in Breast Cancers from Four Different Ethnicities

Jennifer Pollard, Phil A Burns, Tom A Hughes, Colan Ho-Yen, J Louise Jones, Geetashree Mukherjee, Ganiat O Omoniyi-Esan, Nicholas Akinwale Titloye, Valerie Speirs, Abeer M Shaaban

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Abstract

INTRODUCTION: Breast cancer outcomes vary across different ethnic groups. MicroRNAs (miRs) are small non-coding RNA molecules that regulate gene expression across a range of pathologies, including breast cancer. The aim of this study was to evaluate the presence and expression of miRs in breast cancer samples from different ethnic groups.

MATERIALS AND METHODS: Breast cancer tissue from 4 ethnic groups, i.e., British Caucasian, British Black, Nigerian, and Indian, were identified and matched for patients' age, tumour grade/type, and 10 × 10 µm sections taken. Tumour areas were macrodissected, total RNA was extracted, and cDNA was synthesised. cDNA was applied to human miScript PCR arrays allowing the quantification of 84 of the most abundantly expressed/best-characterised miRs.

RESULTS: Differential expression of 9 miRs was seen across the 4 groups. Significantly higher levels of miR-140-5p, miR-194 and miR-423-5p (the last of which harbours the single-nucleotide polymorphism rs6505162) were seen in the breast tumours of Nigerian patients when compared with other ethnic groups (all p < 0.0001). miR-101 was overexpressed in breast cancers in the Indian patients. An in silico analysis of miR-423-5p showed that the AC genotype is mainly associated with Europeans (57%), while Asians display mostly CC (approx. 60%), and Africans mainly AA (approx. 60%).

CONCLUSIONS: This study shows divergence in miR expression in breast cancers from different ethnic groups, and suggests that specific genetic variants in miR genes may affect breast cancer risk in these groups. Predicted targets of these miRs may uncover useful biomarkers that could have clinical value in breast cancers in different ethnic groups.

Original languageEnglish
Pages (from-to)220-226
Number of pages7
JournalPathobiology
Volume85
Issue number4
Early online date23 May 2018
DOIs
Publication statusPublished - 31 Aug 2018

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MicroRNAs
Breast Neoplasms
Ethnic Groups
Complementary DNA
Small Untranslated RNA
Computer Simulation
Single Nucleotide Polymorphism
Neoplasms
Biomarkers
Genotype
RNA
Pathology
Gene Expression
Polymerase Chain Reaction
Genes

Keywords

  • Journal Article
  • breast cancer
  • MicroRNA
  • ethnic background

Cite this

Pollard, J., Burns, P. A., Hughes, T. A., Ho-Yen, C., Jones, J. L., Mukherjee, G., ... Shaaban, A. M. (2018). Differential Expression of MicroRNAs in Breast Cancers from Four Different Ethnicities. Pathobiology, 85(4), 220-226. https://doi.org/10.1159/000488456

Differential Expression of MicroRNAs in Breast Cancers from Four Different Ethnicities. / Pollard, Jennifer; Burns, Phil A; Hughes, Tom A; Ho-Yen, Colan; Jones, J Louise; Mukherjee, Geetashree; Omoniyi-Esan, Ganiat O; Titloye, Nicholas Akinwale; Speirs, Valerie; Shaaban, Abeer M.

In: Pathobiology, Vol. 85, No. 4, 31.08.2018, p. 220-226.

Research output: Contribution to journalArticle

Pollard, J, Burns, PA, Hughes, TA, Ho-Yen, C, Jones, JL, Mukherjee, G, Omoniyi-Esan, GO, Titloye, NA, Speirs, V & Shaaban, AM 2018, 'Differential Expression of MicroRNAs in Breast Cancers from Four Different Ethnicities', Pathobiology, vol. 85, no. 4, pp. 220-226. https://doi.org/10.1159/000488456
Pollard J, Burns PA, Hughes TA, Ho-Yen C, Jones JL, Mukherjee G et al. Differential Expression of MicroRNAs in Breast Cancers from Four Different Ethnicities. Pathobiology. 2018 Aug 31;85(4):220-226. https://doi.org/10.1159/000488456
Pollard, Jennifer ; Burns, Phil A ; Hughes, Tom A ; Ho-Yen, Colan ; Jones, J Louise ; Mukherjee, Geetashree ; Omoniyi-Esan, Ganiat O ; Titloye, Nicholas Akinwale ; Speirs, Valerie ; Shaaban, Abeer M. / Differential Expression of MicroRNAs in Breast Cancers from Four Different Ethnicities. In: Pathobiology. 2018 ; Vol. 85, No. 4. pp. 220-226.
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abstract = "INTRODUCTION: Breast cancer outcomes vary across different ethnic groups. MicroRNAs (miRs) are small non-coding RNA molecules that regulate gene expression across a range of pathologies, including breast cancer. The aim of this study was to evaluate the presence and expression of miRs in breast cancer samples from different ethnic groups.MATERIALS AND METHODS: Breast cancer tissue from 4 ethnic groups, i.e., British Caucasian, British Black, Nigerian, and Indian, were identified and matched for patients' age, tumour grade/type, and 10 × 10 µm sections taken. Tumour areas were macrodissected, total RNA was extracted, and cDNA was synthesised. cDNA was applied to human miScript PCR arrays allowing the quantification of 84 of the most abundantly expressed/best-characterised miRs.RESULTS: Differential expression of 9 miRs was seen across the 4 groups. Significantly higher levels of miR-140-5p, miR-194 and miR-423-5p (the last of which harbours the single-nucleotide polymorphism rs6505162) were seen in the breast tumours of Nigerian patients when compared with other ethnic groups (all p < 0.0001). miR-101 was overexpressed in breast cancers in the Indian patients. An in silico analysis of miR-423-5p showed that the AC genotype is mainly associated with Europeans (57{\%}), while Asians display mostly CC (approx. 60{\%}), and Africans mainly AA (approx. 60{\%}).CONCLUSIONS: This study shows divergence in miR expression in breast cancers from different ethnic groups, and suggests that specific genetic variants in miR genes may affect breast cancer risk in these groups. Predicted targets of these miRs may uncover useful biomarkers that could have clinical value in breast cancers in different ethnic groups.",
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T1 - Differential Expression of MicroRNAs in Breast Cancers from Four Different Ethnicities

AU - Pollard, Jennifer

AU - Burns, Phil A

AU - Hughes, Tom A

AU - Ho-Yen, Colan

AU - Jones, J Louise

AU - Mukherjee, Geetashree

AU - Omoniyi-Esan, Ganiat O

AU - Titloye, Nicholas Akinwale

AU - Speirs, Valerie

AU - Shaaban, Abeer M

N1 - This study was supported by Yorkshire Cancer Research (PP016).

PY - 2018/8/31

Y1 - 2018/8/31

N2 - INTRODUCTION: Breast cancer outcomes vary across different ethnic groups. MicroRNAs (miRs) are small non-coding RNA molecules that regulate gene expression across a range of pathologies, including breast cancer. The aim of this study was to evaluate the presence and expression of miRs in breast cancer samples from different ethnic groups.MATERIALS AND METHODS: Breast cancer tissue from 4 ethnic groups, i.e., British Caucasian, British Black, Nigerian, and Indian, were identified and matched for patients' age, tumour grade/type, and 10 × 10 µm sections taken. Tumour areas were macrodissected, total RNA was extracted, and cDNA was synthesised. cDNA was applied to human miScript PCR arrays allowing the quantification of 84 of the most abundantly expressed/best-characterised miRs.RESULTS: Differential expression of 9 miRs was seen across the 4 groups. Significantly higher levels of miR-140-5p, miR-194 and miR-423-5p (the last of which harbours the single-nucleotide polymorphism rs6505162) were seen in the breast tumours of Nigerian patients when compared with other ethnic groups (all p < 0.0001). miR-101 was overexpressed in breast cancers in the Indian patients. An in silico analysis of miR-423-5p showed that the AC genotype is mainly associated with Europeans (57%), while Asians display mostly CC (approx. 60%), and Africans mainly AA (approx. 60%).CONCLUSIONS: This study shows divergence in miR expression in breast cancers from different ethnic groups, and suggests that specific genetic variants in miR genes may affect breast cancer risk in these groups. Predicted targets of these miRs may uncover useful biomarkers that could have clinical value in breast cancers in different ethnic groups.

AB - INTRODUCTION: Breast cancer outcomes vary across different ethnic groups. MicroRNAs (miRs) are small non-coding RNA molecules that regulate gene expression across a range of pathologies, including breast cancer. The aim of this study was to evaluate the presence and expression of miRs in breast cancer samples from different ethnic groups.MATERIALS AND METHODS: Breast cancer tissue from 4 ethnic groups, i.e., British Caucasian, British Black, Nigerian, and Indian, were identified and matched for patients' age, tumour grade/type, and 10 × 10 µm sections taken. Tumour areas were macrodissected, total RNA was extracted, and cDNA was synthesised. cDNA was applied to human miScript PCR arrays allowing the quantification of 84 of the most abundantly expressed/best-characterised miRs.RESULTS: Differential expression of 9 miRs was seen across the 4 groups. Significantly higher levels of miR-140-5p, miR-194 and miR-423-5p (the last of which harbours the single-nucleotide polymorphism rs6505162) were seen in the breast tumours of Nigerian patients when compared with other ethnic groups (all p < 0.0001). miR-101 was overexpressed in breast cancers in the Indian patients. An in silico analysis of miR-423-5p showed that the AC genotype is mainly associated with Europeans (57%), while Asians display mostly CC (approx. 60%), and Africans mainly AA (approx. 60%).CONCLUSIONS: This study shows divergence in miR expression in breast cancers from different ethnic groups, and suggests that specific genetic variants in miR genes may affect breast cancer risk in these groups. Predicted targets of these miRs may uncover useful biomarkers that could have clinical value in breast cancers in different ethnic groups.

KW - Journal Article

KW - breast cancer

KW - MicroRNA

KW - ethnic background

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EP - 226

JO - Pathobiology

JF - Pathobiology

SN - 1015-2008

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