Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant

Nest McKain; Buğra Genc; Timothy J. Snelling; R. John Wallace

doi:10.1016/j.mimet.2013.10.009

Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant

Nest McKain, Buğra Genc, Timothy J. Snelling, R. John Wallace

The Rowett Institute of Nutrition and Health

Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Animal Nutrition and Nutritional Diseases

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Bacteria and archaea in frozen (− 20 °C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected.

Original language	English
Pages (from-to)	381-383
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	95
Issue number	3
Early online date	24 Oct 2013
DOIs	https://doi.org/10.1016/j.mimet.2013.10.009
Publication status	Published - Dec 2013

Keywords

cryoprotection
glycerol
rumen
storage

Access to Document

10.1016/j.mimet.2013.10.009Licence: Unspecified

Cite this

@article{37d551ca8c0e46f8a666461cb562ec3b,

title = "Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant",

abstract = "Bacteria and archaea in frozen (− 20 °C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected.",

keywords = "cryoprotection, glycerol, rumen, storage",

author = "Nest McKain and Buğra Genc and Snelling, {Timothy J.} and Wallace, {R. John}",

year = "2013",

month = dec,

doi = "10.1016/j.mimet.2013.10.009",

language = "English",

volume = "95",

pages = "381--383",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant

AU - McKain, Nest

AU - Genc, Buğra

AU - Snelling, Timothy J.

AU - Wallace, R. John

PY - 2013/12

Y1 - 2013/12

N2 - Bacteria and archaea in frozen (− 20 °C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected.

AB - Bacteria and archaea in frozen (− 20 °C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected.

KW - cryoprotection

KW - glycerol

KW - rumen

KW - storage

U2 - 10.1016/j.mimet.2013.10.009

DO - 10.1016/j.mimet.2013.10.009

M3 - Article

SN - 0167-7012

VL - 95

SP - 381

EP - 383

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -

Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant

Abstract

Keywords

Access to Document

Fingerprint

Cite this