Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant

Nest McKain, Buğra Genc, Timothy J. Snelling, R. John Wallace

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Bacteria and archaea in frozen (− 20 °C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected.
Original languageEnglish
Pages (from-to)381-383
Number of pages3
JournalJournal of Microbiological Methods
Volume95
Issue number3
Early online date24 Oct 2013
DOIs
Publication statusPublished - Dec 2013

Fingerprint

Bacteroidetes
Archaea
rRNA Genes
Glycerol
Organism Cloning
Bacteria
Firmicutes

Keywords

  • cryoprotection
  • glycerol
  • rumen
  • storage

Cite this

Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant. / McKain, Nest; Genc, Buğra; Snelling, Timothy J.; Wallace, R. John.

In: Journal of Microbiological Methods, Vol. 95, No. 3, 12.2013, p. 381-383.

Research output: Contribution to journalArticle

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