Bacteria and archaea in frozen (− 20 °C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected.
|Number of pages||3|
|Journal||Journal of Microbiological Methods|
|Early online date||24 Oct 2013|
|Publication status||Published - Dec 2013|