Differential response of non-adapted ammonia oxidising archaea and bacteria to drying rewetting stress

Cécile Thion, James I Prosser

Research output: Contribution to journalArticle

34 Citations (Scopus)
5 Downloads (Pure)

Abstract

Climate change is expected to increase the frequency of severe drought events followed by heavy rainfall, which will influence growth and activity of soil microorganisms, through osmotic stress and changes in nutrient concentration. There is evidence of rapid recovery of processes and adaptation of communities in soils regularly experiencing drying/rewetting and lower resistance and resilience in non-adapted soils. A microcosm-based study of ammonia-oxidising archaea (AOA) and bacteria (AOB), employing a grassland soil that rarely experiences drought, was used to test this hypothesis and also whether AOB were more resistant and resilient, through greater tolerance of high ammonia concentrations produced during drought and rewetting. Treated soils were dried, incubated for three weeks, rewetted, incubated for a further three weeks and compared to untreated soils, maintained at a constant moisture content. Nitrate accumulation and AOA and AOB abundance (abundance of respective amoA genes) and community composition (DGGE analysis of AOA amoA and AOB 16S rRNA genes) were poorly adapted to drying-rewetting. AOA abundance and community composition were less resistant than AOB during drought and less resilient after rewetting, at times when ammonium concentration was higher. Data provide evidence for poor adaptation of microbial communities and processes to drying-rewetting in soils with no history of drought and indicate niche differentiation of AOA and AOB associated with high ammonia concentration. This article is protected by copyright. All rights reserved.

Original languageEnglish
Pages (from-to)380-389
Number of pages10
JournalFEMS Microbiology Ecology
Volume90
Issue number2
Early online date15 Sep 2014
DOIs
Publication statusPublished - 1 Nov 2014

Fingerprint

Archaea
Ammonia
Droughts
Soil
Bacteria
Climate Change
Osmotic Pressure
Ammonium Compounds
rRNA Genes
Nitrates
Food
Growth
Genes

Keywords

  • ammonia oxidising archaea
  • Ammonia oxidising bacteria
  • nitrification
  • drought
  • rewetting
  • resistance
  • resilience

Cite this

Differential response of non-adapted ammonia oxidising archaea and bacteria to drying rewetting stress. / Thion, Cécile; Prosser, James I.

In: FEMS Microbiology Ecology, Vol. 90, No. 2, 01.11.2014, p. 380-389.

Research output: Contribution to journalArticle

@article{d6048265dc7f4fdc9ca7dad1d5f19bec,
title = "Differential response of non-adapted ammonia oxidising archaea and bacteria to drying rewetting stress",
abstract = "Climate change is expected to increase the frequency of severe drought events followed by heavy rainfall, which will influence growth and activity of soil microorganisms, through osmotic stress and changes in nutrient concentration. There is evidence of rapid recovery of processes and adaptation of communities in soils regularly experiencing drying/rewetting and lower resistance and resilience in non-adapted soils. A microcosm-based study of ammonia-oxidising archaea (AOA) and bacteria (AOB), employing a grassland soil that rarely experiences drought, was used to test this hypothesis and also whether AOB were more resistant and resilient, through greater tolerance of high ammonia concentrations produced during drought and rewetting. Treated soils were dried, incubated for three weeks, rewetted, incubated for a further three weeks and compared to untreated soils, maintained at a constant moisture content. Nitrate accumulation and AOA and AOB abundance (abundance of respective amoA genes) and community composition (DGGE analysis of AOA amoA and AOB 16S rRNA genes) were poorly adapted to drying-rewetting. AOA abundance and community composition were less resistant than AOB during drought and less resilient after rewetting, at times when ammonium concentration was higher. Data provide evidence for poor adaptation of microbial communities and processes to drying-rewetting in soils with no history of drought and indicate niche differentiation of AOA and AOB associated with high ammonia concentration. This article is protected by copyright. All rights reserved.",
keywords = "ammonia oxidising archaea, Ammonia oxidising bacteria, nitrification, drought, rewetting, resistance, resilience",
author = "C{\'e}cile Thion and Prosser, {James I}",
note = "We would like to acknowledge funding of the European Commission's FP7 programme, EU-project ‘EcoFINDERS’ No. 264465. The authors also wish to thank Dr Franciska De Vries and Professor Richard Bardgett, University of Manchester and Prof Paul Hallett, University of Aberdeen, for their valuable comments and advice.",
year = "2014",
month = "11",
day = "1",
doi = "10.1111/1574-6941.12395",
language = "English",
volume = "90",
pages = "380--389",
journal = "FEMS Microbiology Ecology",
issn = "1574-6941",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Differential response of non-adapted ammonia oxidising archaea and bacteria to drying rewetting stress

AU - Thion, Cécile

AU - Prosser, James I

N1 - We would like to acknowledge funding of the European Commission's FP7 programme, EU-project ‘EcoFINDERS’ No. 264465. The authors also wish to thank Dr Franciska De Vries and Professor Richard Bardgett, University of Manchester and Prof Paul Hallett, University of Aberdeen, for their valuable comments and advice.

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Climate change is expected to increase the frequency of severe drought events followed by heavy rainfall, which will influence growth and activity of soil microorganisms, through osmotic stress and changes in nutrient concentration. There is evidence of rapid recovery of processes and adaptation of communities in soils regularly experiencing drying/rewetting and lower resistance and resilience in non-adapted soils. A microcosm-based study of ammonia-oxidising archaea (AOA) and bacteria (AOB), employing a grassland soil that rarely experiences drought, was used to test this hypothesis and also whether AOB were more resistant and resilient, through greater tolerance of high ammonia concentrations produced during drought and rewetting. Treated soils were dried, incubated for three weeks, rewetted, incubated for a further three weeks and compared to untreated soils, maintained at a constant moisture content. Nitrate accumulation and AOA and AOB abundance (abundance of respective amoA genes) and community composition (DGGE analysis of AOA amoA and AOB 16S rRNA genes) were poorly adapted to drying-rewetting. AOA abundance and community composition were less resistant than AOB during drought and less resilient after rewetting, at times when ammonium concentration was higher. Data provide evidence for poor adaptation of microbial communities and processes to drying-rewetting in soils with no history of drought and indicate niche differentiation of AOA and AOB associated with high ammonia concentration. This article is protected by copyright. All rights reserved.

AB - Climate change is expected to increase the frequency of severe drought events followed by heavy rainfall, which will influence growth and activity of soil microorganisms, through osmotic stress and changes in nutrient concentration. There is evidence of rapid recovery of processes and adaptation of communities in soils regularly experiencing drying/rewetting and lower resistance and resilience in non-adapted soils. A microcosm-based study of ammonia-oxidising archaea (AOA) and bacteria (AOB), employing a grassland soil that rarely experiences drought, was used to test this hypothesis and also whether AOB were more resistant and resilient, through greater tolerance of high ammonia concentrations produced during drought and rewetting. Treated soils were dried, incubated for three weeks, rewetted, incubated for a further three weeks and compared to untreated soils, maintained at a constant moisture content. Nitrate accumulation and AOA and AOB abundance (abundance of respective amoA genes) and community composition (DGGE analysis of AOA amoA and AOB 16S rRNA genes) were poorly adapted to drying-rewetting. AOA abundance and community composition were less resistant than AOB during drought and less resilient after rewetting, at times when ammonium concentration was higher. Data provide evidence for poor adaptation of microbial communities and processes to drying-rewetting in soils with no history of drought and indicate niche differentiation of AOA and AOB associated with high ammonia concentration. This article is protected by copyright. All rights reserved.

KW - ammonia oxidising archaea

KW - Ammonia oxidising bacteria

KW - nitrification

KW - drought

KW - rewetting

KW - resistance

KW - resilience

U2 - 10.1111/1574-6941.12395

DO - 10.1111/1574-6941.12395

M3 - Article

VL - 90

SP - 380

EP - 389

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 1574-6941

IS - 2

ER -