Differentially expressed genes in the intestinal tract of the blood sucking bug, Triatoma infestans

A. H. Kollien, Peter Francis Billingsley

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    Abstract

    An arbitrary-primed RNA PCR differential display strategy was used to identify midgut genes of the reduviid bug Triatoma infestans that were differentially expressed after a blood meal. From interesting bands, 33 distinct cDNAs were cloned and sequenced. Although many had long open reading frames, most of the transcripts were unrelated to any other sequences in any databases. Only 14 Triatoma sequences had strong homologies to those from other organisms, including genes encoding for 2-oxoglutarate dehydrogenase, CAD protein, NADH-ubiquinone-oxoreductase, epidermal growth factor, plectin, aminopeptidase, heat-shock-related 70-kDa protein, golgin, mitochondrial carrier protein and high-density lipoprotein. RT-PCR was used to demonstrate constitutive expression in four of five of these sequences. Northern hybridisation was difficult due to the very low expression levels of most of the genes. However, a gene-fragment highly homologous to a heat-shock-related 70-kDa protein was strongly expressed in starved bugs, down-regulated after feeding and again expressed later, suggesting a role for a heat-shock protein in starvation survival.

    Original languageEnglish
    Pages (from-to)1026-1033
    Number of pages7
    JournalParasitology Research
    Volume88
    DOIs
    Publication statusPublished - 2002

    Keywords

    • PROLIXUS STAL HEMIPTERA
    • RHODNIUS-PROLIXUS
    • TRYPANOSOMA-CRUZI
    • MESSENGER-RNA
    • POSTERIOR MIDGUT
    • ANOPHELES-GAMBIAE
    • BLASTOCRITHIDIA-TRIATOMAE
    • SARCOPHAGA-CRASSIPALPIS
    • DIGESTIVE PROTEINASES
    • PUPAL DIAPAUSE

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