Abstract
PCR-based methods have been developed to differentiate four species of commercially important eels (Anguilla anguilla, A. rostrata, A. japonica, A. australis). By means of universal primers a 464 base pair (bp) section of the cytochrome b gene was amplified, The amplicon was sequenced and restriction enzymes were selected for RFLP analysis a short part ( 123 bp) of the 464 bp section was amplified by another set of primers and used for SSCP analysis.
The suitability of RFLP and SSCP for differentiating hot-smoked eel was verified by a collaborative study.
Original language | English |
---|---|
Pages (from-to) | 171-177 |
Number of pages | 6 |
Journal | European Food Research and Technology |
Volume | 214 |
Issue number | 2 |
DOIs | |
Publication status | Published - Feb 2002 |
Keywords
- eel
- species identification
- PCR-RFLP
- PCR-SSCP
- fresh-water eels
- mitochondrial-DNA
- anguilla-anguilla
- phylogenetic-relationships
- genus anguilla
- evolution
- variability
- Australis
- products