Direct Enzymatic Detection of Endogenous Oxidative Base Damage in Human Lymphocyte DNA

A R Collins, Susan Joyce Duthie, V L Dobson

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    Abstract

    The endogenous production of oxidative damage in DNA by free radicals released as a by-product of respiration is a likely cause of mutations which, if they occur in appropriate genes, may lead to cancer. Using an endonuclease specific for oxidized pyrimidines, in conjunction with the highly sensitive method of single cell gel electrophoresis, we have detected significant oxidative damage in untreated, freshly isolated lymphocytes from normal, healthy individuals.

    Original languageEnglish
    Pages (from-to)1733-1735
    Number of pages3
    JournalCarcinogenesis
    Volume14
    Issue number9
    Publication statusPublished - Sep 1993

    Keywords

    • Escherichia-Coli
    • human-cells
    • repair
    • assay

    Cite this

    Collins, A. R., Duthie, S. J., & Dobson, V. L. (1993). Direct Enzymatic Detection of Endogenous Oxidative Base Damage in Human Lymphocyte DNA. Carcinogenesis, 14(9), 1733-1735.