Direct Enzymatic Detection of Endogenous Oxidative Base Damage in Human Lymphocyte DNA

A R  Collins; Susan Joyce Duthie; V L  Dobson

Direct Enzymatic Detection of Endogenous Oxidative Base Damage in Human Lymphocyte DNA

A R Collins, Susan Joyce Duthie, V L Dobson

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Article › peer-review

779 Citations (Scopus)

Abstract

The endogenous production of oxidative damage in DNA by free radicals released as a by-product of respiration is a likely cause of mutations which, if they occur in appropriate genes, may lead to cancer. Using an endonuclease specific for oxidized pyrimidines, in conjunction with the highly sensitive method of single cell gel electrophoresis, we have detected significant oxidative damage in untreated, freshly isolated lymphocytes from normal, healthy individuals.

Original language	English
Pages (from-to)	1733-1735
Number of pages	3
Journal	Carcinogenesis
Volume	14
Issue number	9
Publication status	Published - Sept 1993

Keywords

Escherichia-Coli
human-cells
repair
assay

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Direct Enzymatic Detection of Endogenous Oxidative Base Damage in Human Lymphocyte DNA",

abstract = "The endogenous production of oxidative damage in DNA by free radicals released as a by-product of respiration is a likely cause of mutations which, if they occur in appropriate genes, may lead to cancer. Using an endonuclease specific for oxidized pyrimidines, in conjunction with the highly sensitive method of single cell gel electrophoresis, we have detected significant oxidative damage in untreated, freshly isolated lymphocytes from normal, healthy individuals.",

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year = "1993",

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T1 - Direct Enzymatic Detection of Endogenous Oxidative Base Damage in Human Lymphocyte DNA

AU - Collins, A R

AU - Duthie, Susan Joyce

AU - Dobson, V L

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Y1 - 1993/9

N2 - The endogenous production of oxidative damage in DNA by free radicals released as a by-product of respiration is a likely cause of mutations which, if they occur in appropriate genes, may lead to cancer. Using an endonuclease specific for oxidized pyrimidines, in conjunction with the highly sensitive method of single cell gel electrophoresis, we have detected significant oxidative damage in untreated, freshly isolated lymphocytes from normal, healthy individuals.

AB - The endogenous production of oxidative damage in DNA by free radicals released as a by-product of respiration is a likely cause of mutations which, if they occur in appropriate genes, may lead to cancer. Using an endonuclease specific for oxidized pyrimidines, in conjunction with the highly sensitive method of single cell gel electrophoresis, we have detected significant oxidative damage in untreated, freshly isolated lymphocytes from normal, healthy individuals.

KW - Escherichia-Coli

KW - human-cells

KW - repair

KW - assay

M3 - Article

SN - 0143-3334

VL - 14

SP - 1733

EP - 1735

JO - Carcinogenesis

JF - Carcinogenesis

IS - 9

ER -

Direct Enzymatic Detection of Endogenous Oxidative Base Damage in Human Lymphocyte DNA

Abstract

Keywords

UN SDGs

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