Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells

Maria Grazia Cascio; Pietro Marini; Roger G. Pertwee

doi:10.1007/978-1-0716-2728-0_8

Displacement Binding Assay Using Human Cannabinoid CB₂ Receptor-Transfected Cells

Maria Grazia Cascio, Pietro Marini, Roger G. Pertwee^* (Corresponding Author)

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Displacement binding assays are nonfunctional assays mostly used with the aim of determining whether a certain compound (plant-derived or synthetic) can bind to a specific receptor with high affinity. Here, we describe the displacement binding assay that is carried out with a radioligand and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB₂ receptor.

Original language	English
Title of host publication	Endocannabinoid Signaling.
Subtitle of host publication	Methods in Molecular Biology
Editors	M. Maccarrone
Place of Publication	New York
Publisher	HUMANA PRESS INC
Pages	111-118
Number of pages	8
Volume	2576
ISBN (Electronic)	978-1-0716-2728-0
ISBN (Print)	978-1-0716-2727-3
DOIs	https://doi.org/10.1007/978-1-0716-2728-0_8
Publication status	Published - 9 Oct 2023

Publication series

Name	Methods in Molecular Biology
Volume	2576
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Affinity
Agonist
Antagonist
Binding
Cannabinoid CB receptors
Ligand
Radioligand

Access to Document

10.1007/978-1-0716-2728-0_8Licence: Unspecified

Cite this

Displacement Binding Assay Using Human Cannabinoid CB₂ Receptor-Transfected Cells. / Cascio, Maria Grazia ; Marini, Pietro ; Pertwee, Roger G. (Corresponding Author).
Endocannabinoid Signaling. : Methods in Molecular Biology. ed. / M. Maccarrone. Vol. 2576 New York: HUMANA PRESS INC, 2023. p. 111-118 (Methods in Molecular Biology; Vol. 2576).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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