Dissection of the Candida albicans class I chitin synthase promoters

Megan D. Lenardon, Iwona Lesiak, Carol A. Munro, Neil A. R. Gow

Research output: Contribution to journalArticle

19 Citations (Scopus)
8 Downloads (Pure)

Abstract

Maintenance of the integrity of the cell wall in fungi is essential. One mechanism that cells use to maintain cell wall integrity in response to cell wall damage is to up-regulate chitin synthesis. In Candida albicans, the PKC cell wall integrity, Ca2+/calcineurin and high osmolarity glycerol (HOG) signalling pathways co-ordinately regulate chitin synthesis in response to cell wall stress. The transcription factors downstream of these pathways and their DNA binding sites within the promoters of target genes are well characterised in Saccharomyces cerevisiae, but not in C. albicans. The promoters of the C. albicans class I CHS genes (CaCHS2 and CaCHS8) were functionally dissected with the aim of identifying and characterising the transcription factors and promoter elements that mediate the transcriptional up-regulation of CaCHS2 and CaCHS8 in response to cell wall stress. This analysis provided evidence that the PKC cell wall integrity pathway may operate through RLM1-elements in the CaCHS2 and CaCHS8 promoters, but that promoter sequences that respond to the Ca2+/calcineurin and HOG signalling pathways in S. cerevisiae did not directly regulate chitin synthase 2 and 8 gene transcription in C. albicans.

Original languageEnglish
Pages (from-to)459-471
Number of pages13
JournalMolecular Genetics and Genomics: MGG
Volume281
Issue number4
Early online date20 Jan 2009
DOIs
Publication statusPublished - Apr 2009

Keywords

  • base sequence
  • binding sites
  • Candida albicans
  • cell wall
  • chitin synthase
  • DNA primers
  • DNA, fungal
  • genes, fungal
  • molecular sequence data
  • mutagenesis, site-directed
  • promoter regions, genetic
  • signal transduction
  • transcription factors
  • chitin
  • transcription

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