Distinct expression patterns of ERα and ERβ in normal human mammary gland

V. Speirs, G. P. Skliris, S. E. Burdall, P. J. Carder

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Aim: Two oestrogen receptors (ERs) have been identified to date-the "classic" ERα and the more recently described ERβ. Although much is known about ERα at the mRNA and protein levels, our knowledge of the expression and distribution of ERβ protein is much more limited. The aim of this study was to compare the cellular distribution of ERα and ERβ in normal human mammary gland. Methods: Formalin fixed, paraffin wax embedded material was obtained from reduction mammoplasty specimens, normal tissue adjacent to breast tumour, or fibroadenoma. Sections were immunohistochemically stained for ERα, ERβ, and the progesterone receptor. The staining pattern for each antibody was evaluated and compared. Results: ERα was restricted to the cell nuclei of epithelial cells lining ducts and lobules. Although ERβ was also seen in these cells, additional strong staining was detected specifically in the cell nuclei of myoepithelial cells. Occasional staining was seen in surrounding stromal and endothelial cell nuclei and in lymphocytes. Conclusions: ER subtypes have distinct distribution patterns in the normal mammary gland. The widespread distribution of ERβ suggests that it may be the dominant ER in the mammary gland where it may be acting as a natural suppressor.

Original languageEnglish
Pages (from-to)371-374
Number of pages4
JournalJournal of Clinical Pathology
Volume55
Issue number5
DOIs
Publication statusPublished - 2002

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Human Mammary Glands
Cell Nucleus
Staining and Labeling
Fibroadenoma
Mammaplasty
Waxes
Progesterone Receptors
Stromal Cells
Estrogen Receptors
Paraffin
Formaldehyde
Proteins
Endothelial Cells
Epithelial Cells
Lymphocytes
Breast Neoplasms
Messenger RNA
Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Distinct expression patterns of ERα and ERβ in normal human mammary gland. / Speirs, V.; Skliris, G. P.; Burdall, S. E.; Carder, P. J.

In: Journal of Clinical Pathology, Vol. 55, No. 5, 2002, p. 371-374.

Research output: Contribution to journalArticle

Speirs, V. ; Skliris, G. P. ; Burdall, S. E. ; Carder, P. J. / Distinct expression patterns of ERα and ERβ in normal human mammary gland. In: Journal of Clinical Pathology. 2002 ; Vol. 55, No. 5. pp. 371-374.
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